Small Angle Scattering Biological Data Bank SASBDB

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33 hits found for Kursula

SASDB52 – Death associated protein kinase (wild-type)

Death associated protein kinase wild-type experimental SAS data

Sample:	Death associated protein kinase wild-type , 37 kDa Homo sapiens protein
Buffer:	50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18

Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M

R_g^Guinier	2.7	nm
D_max	8.1	nm
Volume^Porod	101	nm³

SASDB62 – Death associated protein kinase (D220K mutant)

Death associated protein kinase (D220K mutant) experimental SAS data

Sample:	Death associated protein kinase (D220K mutant) , 37 kDa Homo sapiens protein
Buffer:	50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18

R_g^Guinier	2.5	nm
D_max	9.0	nm
Volume^Porod	89	nm³

SASDB72 – Death associated protein kinase (Basic Loop mutant)

Death associated protein kinase (Basic Loop mutant) experimental SAS data

Sample:	Death associated protein kinase (Basic Loop mutant) , 37 kDa Homo sapiens protein
Buffer:	50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18

R_g^Guinier	2.4	nm
D_max	7.5	nm
Volume^Porod	78	nm³

SASDAN4 – Calmodulin:peptide complex

CalmodulinC-terminal region of human myelin basic protein experimental SAS data

Sample:	Calmodulin monomer, 17 kDa Homo sapiens protein C-terminal region of human myelin basic protein monomer, 2 kDa Homo sapiens protein
Buffer:	25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 28

Interaction between the C-terminal region of human myelin basic protein and calmodulin: analysis of complex formation and solution structure. BMC Struct Biol 8:10 (2008)
...Kursula P

R_g^Guinier	2.1	nm
D_max	7.0	nm
Volume^Porod	36	nm³

SASDBZ4 – Tyrosine hydroxylase (Isoform 1, Homo sapiens)

Tyrosine hydroxylase, isoform 1 experimental SAS data

Sample:	Tyrosine hydroxylase, isoform 1 tetramer, 222 kDa Homo sapiens protein
Buffer:	20 mM Na-HEPES 200 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 14

Stable preparations of tyrosine hydroxylase provide the solution structure of the full-length enzyme. Sci Rep 6:30390 (2016)
...Kursula P, Martinez A, Flydal MI

R_g^Guinier	4.7	nm
D_max	20.0	nm
Volume^Porod	520	nm³

SASDLA5 – Wild-type human myelin protein P2

Sample:	Myelin P2 protein monomer, 15 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jul 22

Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants. FEBS J (2021)
...Kursula P

R_g^Guinier	1.5	nm
D_max	4.2	nm
Volume^Porod	17	nm³

SASDLB5 – CMT mutant i50del of human myelin protein P2

Myelin P2 disease mutant I50del experimental SAS data

Sample:	Myelin P2 disease mutant I50del monomer, 15 kDa protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jul 22

Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants. FEBS J (2021)
...Kursula P

R_g^Guinier	1.5	nm
D_max	4.0	nm
Volume^Porod	17	nm³

SASDLC5 – CMT mutant M114T of human myelin protein P2

Human P2 M114T mutant experimental SAS data

Sample:	Human P2 M114T mutant monomer, 15 kDa protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jul 22

Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants. FEBS J (2021)
...Kursula P

R_g^Guinier	1.5	nm
D_max	4.0	nm
Volume^Porod	16	nm³

SASDLD5 – CMT mutant V115A of human myelin protein P2

Human P2 V115A mutant experimental SAS data

Sample:	Human P2 V115A mutant monomer, 15 kDa protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jul 22

Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants. FEBS J (2021)
...Kursula P

R_g^Guinier	1.5	nm
D_max	4.0	nm
Volume^Porod	17	nm³

SASDND6 – Human ganglioside-induced differentiation-associated protein 1 (GDAP1) missense mutant H123R, construct GDAP1∆302-358

Ganglioside-induced differentiation-associated protein 1 (H123R) experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1 (H123R) dimer, 70 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Oct 8

Structural insights into Charcot-Marie-Tooth disease-linked mutations in human GDAP1. FEBS Open Bio (2022)
...Kursula P

R_g^Guinier	3.1	nm
D_max	9.9	nm
Volume^Porod	107	nm³

SASDNE6 – Human ganglioside-induced differentiation-associated protein 1 (GDAP1) missense mutant R120W, construct GDAP1∆302-358

Ganglioside-induced differentiation-associated protein 1 (R120W) experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1 (R120W) dimer, 70 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 1

Structural insights into Charcot-Marie-Tooth disease-linked mutations in human GDAP1. FEBS Open Bio (2022)
...Kursula P

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	105	nm³

SASDJR7 – Mouse cysteine sulphinic acid decarboxylase (CSAD)

Cysteine sulfinic acid decarboxylase experimental SAS data

Sample:	Cysteine sulfinic acid decarboxylase dimer, 117 kDa Mus musculus protein
Buffer:	20 mM HEPES, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Feb 4

Structure and substrate specificity determinants of the taurine biosynthetic enzyme cysteine sulphinic acid decarboxylase. J Struct Biol :107674 (2020)
...Kursula P

R_g^Guinier	3.4	nm
D_max	15.0	nm
Volume^Porod	198	nm³

SASDRZ7 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT)

Nicotinamide phosphoribosyltransferase experimental SAS data

Nicotinamide phosphoribosyltransferase Kratky plot

Sample:	Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.3	nm
D_max	12.9	nm
Volume^Porod	181	nm³

SASDR28 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of nicotinamide

Sample:	Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinamide, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.2	nm
D_max	12.8	nm
Volume^Porod	166	nm³

SASDR38 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of nicotinic acid

Sample:	Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinic acid, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.3	nm
D_max	12.9	nm
Volume^Porod	180	nm³

SASDR48 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of phosphoribosyl pyrophosphate

Sample:	Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM phosphoribosyl pyrophosphate, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.3	nm
D_max	12.9	nm
Volume^Porod	173	nm³

SASDR58 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51)

Nicotinamide phosphoribosyltransferase Δ42-51 experimental SAS data

Nicotinamide phosphoribosyltransferase Δ42-51 Kratky plot

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.2	nm
D_max	11.0	nm
Volume^Porod	159	nm³

SASDR68 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of nicotinamide

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinamide, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.2	nm
D_max	10.9	nm
Volume^Porod	151	nm³

SASDR78 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of nicotinic acid

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinic acid, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.3	nm
D_max	11.2	nm
Volume^Porod	152	nm³

SASDR88 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of phosphoribosyl pyrophosphate

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM phosphoribosyl pyrophosphate, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
...Kursula P, Ziegler M

R_g^Guinier	3.2	nm
D_max	11.4	nm
Volume^Porod	154	nm³

SASDJR8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 dimer, 68 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 21

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	3.1	nm
D_max	101.6	nm
Volume^Porod	111	nm³

SASDJS8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 dimer, 68 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	3.1	nm
D_max	9.2	nm
Volume^Porod	112	nm³

SASDJT8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 monomer, 34 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 21

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	2.7	nm
D_max	92.3	nm
Volume^Porod	71	nm³

SASDJU8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 monomer, 34 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	2.5	nm
D_max	8.9	nm
Volume^Porod	63	nm³

SASDJV8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358 experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358 dimer, 70 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 28

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	3.1	nm
D_max	9.9	nm
Volume^Porod	106	nm³

SASDJW8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A monomer, 35 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 28

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	2.5	nm
D_max	8.7	nm
Volume^Porod	59	nm³

SASDJX8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 dimer, 74 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	3.5	nm
D_max	10.8	nm
Volume^Porod	130	nm³

SASDJY8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 dimer, 74 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	3.1	nm
D_max	9.6	nm
Volume^Porod	121	nm³

SASDJZ8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	2.7	nm
D_max	9.0	nm
Volume^Porod	69	nm³

SASDSZ8 – The DNA binding domain (DBD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

Hepatocyte nuclear factor 1-alpha experimental SAS data

Hepatocyte nuclear factor 1-alpha Kratky plot

Sample:	Hepatocyte nuclear factor 1-alpha monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 1 mM TCEP,, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 25

Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
...Kursula P

R_g^Guinier	2.7	nm
D_max	10.2	nm
Volume^Porod	22	nm³

SASDJ29 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	2.5	nm
D_max	8.6	nm
Volume^Porod	65	nm³

SASDS29 – The DNA binding and transactivation domains (DBD-TAD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

Sample:	Hepatocyte nuclear factor 1-alpha monomer, 62 kDa Homo sapiens protein
Buffer:	4 mM Tris, 100 mM NaCl, 1 mM TCEP,, pH: 8.5
Experiment:	SAXS data collected at CoSAXS, MAX IV on 2022 Apr 2

Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
...Kursula P

R_g^Guinier	4.9	nm
D_max	17.0	nm
Volume^Porod	109	nm³

SASDJ39 – Monomeric human ganglioside-induced differentiation-associated protein 1-like 1, GDAP1L1

Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) monomer, 44 kDa Homo sapiens protein
Buffer:	20 mM TRIS pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 10

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Kursula P

R_g^Guinier	2.7	nm
D_max	10.0	nm
Volume^Porod	73	nm³