Small Angle Scattering Biological Data Bank SASBDB


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21 hits found for Seraphim

SASDBD3 – Leishmania braziliensis heat shock protein 90 (Hsp90).

Leishmania braziliensis heat shock protein 90 (Hsp90) experimental SAS data

Sample:	Leishmania braziliensis heat shock protein 90 (Hsp90) dimer, 166 kDa Leishmania braziliensis protein
Buffer:	25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1

Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering. Int J Biol Macromol 97:503-512 (2017)
Seraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC

R_g^Guinier	5.3	nm
D_max	21.0	nm
Volume^Porod	380	nm³

SASDBE3 – Leishmania braziliensis heat shock protein 90 (Hsp90) N domain.

Leishmania braziliensis heat shock protein 90 (Hsp90) N domain experimental SAS data

Sample:	Leishmania braziliensis heat shock protein 90 (Hsp90) N domain monomer, 26 kDa Leishmania braziliensis protein
Buffer:	25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1

R_g^Guinier	2.0	nm
D_max	7.5	nm
Volume^Porod	40	nm³

SASDBF3 – Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains experimental SAS data

Sample:	Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains monomer, 62 kDa Leishmania braziliensis protein
Buffer:	25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1

R_g^Guinier	3.2	nm
D_max	13.0	nm
Volume^Porod	89	nm³

SASDB54 – Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct

Stress-induced protein sti1 experimental SAS data

Sample:	Stress-induced protein sti1 monomer, 62 kDa Leishmania braziliensis protein
Buffer:	25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21

Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Arch Biochem Biophys 600:12-22 (2016)
...Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC

R_g^Guinier	4.5	nm
D_max	18.0	nm
Volume^Porod	94	nm³

SASDB64 – Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct)

Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) experimental SAS data

Sample:	Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) monomer, 44 kDa Leishmania braziliensis protein
Buffer:	25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	65	nm³

SASDBR4 – Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1)

Activator of Hsp90 ATPase-1 experimental SAS data

Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1) Rg histogram

Sample:	Activator of Hsp90 ATPase-1 monomer, 38 kDa Leishmania braziliensis protein
Buffer:	25 mM Sodium phosphate, 50 mM NaCl, 2 mM EDTA, 1 mM β-mercaptoethanol, pH: 7
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 1

Low resolution structural studies indicate that the activator of Hsp90 ATPase 1 (Aha1) of Leishmania braziliensis has an elongated shape which allows its interaction with both N- and M-domains of Hsp9... PLoS One 8(6):e66822 (2013)
Seraphim TV, Alves MM, Silva IM, Gomes FE, Silva KP, Murta SM, Barbosa LR, Borges JC

R_g^Guinier	3.6	nm
D_max	14.5	nm

SASDC55 – Plasmodium falciparum p23A

CS domain protein, putative experimental SAS data

Sample:	CS domain protein, putative monomer, 19 kDa Plasmodium falciparum protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26

Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. Int J Biol Macromol 108:193-204 (2018)
...Seraphim TV, Minari K, Barbosa LRS, Borges JC

R_g^Guinier	2.5	nm
D_max	8.5	nm

SASDC65 – Plasmodium falciparum p23B

Sample:	Co-chaperone p23 monomer, 31 kDa Plasmodium falciparum protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26

R_g^Guinier	3.7	nm
D_max	13.0	nm

SASDC75 – Leishmania braziliensis SGT co-chaperone

Sample:	SGT protein dimer, 92 kDa Leishmania braziliensis protein
Buffer:	20 mM Potassium Phosphate, 100 mM KCl, 10 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2014 May 14

Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinitie... Int J Biol Macromol 118(Pt A):693-706 (2018)
...Seraphim TV, Batista FAH, Dores-Silva PR, Barranco ABF, Teixeira FR, Gava LM, Borges JC

R_g^Guinier	4.5	nm
D_max	17.0	nm

SASDC85 – Leishmania braziliensis p23B

Leishmania braziliensis p23 isoform B experimental SAS data

Sample:	Leishmania braziliensis p23 isoform B monomer, 23 kDa Leishmania braziliensis protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Mar 26

Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities. FEBS J 282(2):388-406 (2015)
...Seraphim TV, Silva KP, Murta SM, Barbosa LR, Borges JC

R_g^Guinier	3.0	nm
D_max	13.0	nm

SASDFL5 – Plasmodium falciparum Hsp70/Hsp90 organizing protein, Hop

Sample:	STI1-like protein dimer, 136 kDa Plasmodium falciparum protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Aug 3

Structural studies of the Hsp70/Hsp90 organizing protein of Plasmodium falciparum and its modulation of Hsp70 and Hsp90 ATPase activities. Biochim Biophys Acta Proteins Proteom :140282 (2019)
...Seraphim TV, Barbosa LRS, Borges JC

R_g^Guinier	6.3	nm
D_max	24.0	nm
Volume^Porod	557	nm³

SASDCV5 – Leishmania braziliensis p23A

Uncharacterized protein experimental SAS data

Sample:	Uncharacterized protein monomer, 22 kDa Leishmania braziliensis protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Mar 26

R_g^Guinier	3.3	nm
D_max	13.0	nm

SASDR96 – Human DjC20/DnaJC20/HscB iron-sulfur cluster co-chaperone protein

Iron-sulfur cluster co-chaperone protein HscB experimental SAS data

Sample:	Iron-sulfur cluster co-chaperone protein HscB monomer, 25 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 50 mM NaCl, 5 mM KCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 Aug 14

Structural characterization of the human DjC20/HscB cochaperone in solution Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140970 (2023)
...Seraphim T, Borges J

R_g^Guinier	2.5	nm
D_max	9.0	nm
Volume^Porod	40	nm³

SASDQ98 – Full-length human DPCD

Sample:	Protein DPCD monomer, 23 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2018 Nov 8

DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling. Cell Rep 43(2):113713 (2024)
...Seraphim TV, Wan Y, Wu R, Coyaud E, Bin Munim M, Mollica A, Laurent E, Babu M, Mennella V, Raught B, Houry WA

R_g^Guinier	2.1	nm
D_max	7.8	nm
Volume^Porod	28	nm³

SASDHD9 – Full-length Plasmodium falciparum Heat shock protein 90 (PfHsp90)

Plasmodium falciparum Heat shock protein 90 experimental SAS data

Sample:	Plasmodium falciparum Heat shock protein 90 dimer, 177 kDa Plasmodium falciparum protein
Buffer:	25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11

Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
...Seraphim T, Borges J

R_g^Guinier	5.7	nm
D_max	19.0	nm
Volume^Porod	350	nm³

SASDHE9 – Plasmodium falciparum Heat shock protein 90 (PfHsp90) N- and M-domains

Plasmodium falciparum Heat shock protein 90 N-terminal and Middle domains experimental SAS data

Plasmodium falciparum Heat shock protein 90 (PfHsp90) N- and M-domains Rg histogram

Sample:	Plasmodium falciparum Heat shock protein 90 N-terminal and Middle domains monomer, 68 kDa Plasmodium falciparum protein
Buffer:	25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11

Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
...Seraphim T, Borges J

R_g^Guinier	3.9	nm
D_max	14.0	nm
Volume^Porod	106	nm³

SASDHF9 – Plasmodium falciparum Heat shock protein 90 (PfHsp90) M-domain

Plasmodium falciparum Heat shock protein 90 middle domain experimental SAS data

Sample:	Plasmodium falciparum Heat shock protein 90 middle domain monomer, 33 kDa Plasmodium falciparum protein
Buffer:	25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11

Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
...Seraphim T, Borges J

R_g^Guinier	2.4	nm
D_max	8.5	nm
Volume^Porod	47	nm³

SASDBH6 – Full-length human p23 (1-160)

Prostaglandin E synthase 3 experimental SAS data

Sample:	Prostaglandin E synthase 3 monomer, 19 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22

The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
Seraphim TV, Gava LM, Mokry DZ, Cagliari TC, Barbosa LR, Ramos CH, Borges JC

R_g^Guinier	2.5	nm
D_max	10.0	nm
Volume^Porod	40	nm³

SASDBJ6 – Truncated construct of human p23 (1-142)

Prostaglandin E synthase 3 (1-142) experimental SAS data

Prostaglandin E synthase 3 (1-142) Kratky plot

Sample:	Prostaglandin E synthase 3 (1-142) monomer, 17 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22

R_g^Guinier	2.1	nm
D_max	8.5	nm
Volume^Porod	36	nm³

SASDBL6 – Truncated construct of human p23 (1-117)

Prostaglandin E synthase 3 (1-117) experimental SAS data

Prostaglandin E synthase 3 (1-117) Kratky plot

Sample:	Prostaglandin E synthase 3 (1-117) monomer, 14 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 21

R_g^Guinier	1.9	nm
D_max	7.0	nm
Volume^Porod	29	nm³

SASDBK6 – Truncated construct of human p23 (1-131)

Prostaglandin E synthase 3 (1-131) experimental SAS data

Prostaglandin E synthase 3 (1-131) Kratky plot

Sample:	Prostaglandin E synthase 3 (1-131) monomer, 16 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 21

R_g^Guinier	1.9	nm
D_max	7.0	nm
Volume^Porod	31	nm³

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