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10 hits found for Tully

SASDTV5 – Human full-length Annexin A11 (apo form)

Annexin A11 experimental SAS data
Annexin A11 Kratky plot
Sample: Annexin A11 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 5 mM EDTA, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jul 21
The structural properties of full-length annexin A11 Frontiers in Molecular Biosciences 11 (2024)
...Tully M, Foldes T, Kelly G, Tartaglia G, Pastore A
RgGuinier 3.8 nm
Dmax 15.9 nm
VolumePorod 84 nm3

SASDTW5 – Human full-length Annexin A11 (holo form, with calcium)

Annexin A11 experimental SAS data
Annexin A11 Kratky plot
Sample: Annexin A11 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 500 µM CaCl2, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jul 21
The structural properties of full-length annexin A11 Frontiers in Molecular Biosciences 11 (2024)
...Tully M, Foldes T, Kelly G, Tartaglia G, Pastore A
RgGuinier 3.8 nm
Dmax 17.1 nm
VolumePorod 83 nm3

SASDRC6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with ADP-AlF4

Mitogen-activated protein kinase 14Dual specificity mitogen-activated protein kinase kinase 6Adenosine 5'-Diphosphate Tetrafluoroaluminate experimental SAS data
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 Adenosine 5'-Diphosphate Tetrafluoroaluminate Kratky plot
Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein
Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein
Adenosine 5'-Diphosphate Tetrafluoroaluminate monomer, 1 kDa
Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Science 381(6663):1217-1225 (2023)
...Tully M, Vadas O, Gervasio F, Pellegrini E, Bowler M
RgGuinier 3.2 nm
Dmax 12.1 nm
VolumePorod 113 nm3

SASDRD6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with AMP-PCP

Mitogen-activated protein kinase 14Dual specificity mitogen-activated protein kinase kinase 65′‐adenylyl methylenediphosphonate experimental SAS data
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 5′‐adenylyl methylenediphosphonate Kratky plot
Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein
Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein
5′‐adenylyl methylenediphosphonate monomer, 1 kDa
Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Science 381(6663):1217-1225 (2023)
...Tully M, Vadas O, Gervasio F, Pellegrini E, Bowler M
RgGuinier 4.2 nm
Dmax 14.0 nm
VolumePorod 116 nm3

SASDNB8 – Mature full length human prion protein

Major prion protein experimental SAS data
Mature full length human prion protein Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
...Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 2.8 nm
Dmax 10.2 nm

SASDNC8 – Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP]

Major prion protein experimental SAS data
Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP] Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
...Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 3.1 nm
Dmax 11.3 nm

SASDND8 – Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP]

Major prion protein experimental SAS data
Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP] Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
...Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDEV6 – KRAB-associated protein 1 (KAP1); TRIM28; full length protein

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 dimer, 183 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
...Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 9.0 nm
Dmax 38.0 nm
VolumePorod 710 nm3

SASDER7 – KRAB-associated protein 1 (KAP1); TRIM28, amino acids 23-812

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 experimental SAS data
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 Kratky plot
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 dimer, 175 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
...Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 8.9 nm
Dmax 38.0 nm
VolumePorod 853 nm3

SASDEW6 – KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC domain

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain dimer, 92 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
...Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 8.3 nm
Dmax 37.0 nm
VolumePorod 381 nm3