Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDBS6 – Apo form of full length ObgE from E.coli (ObgE_FL)

UniProt ID: P42641 (1-390) GTPase ObgE/CgtA

Apo form of full length ObgE from E.coli (ObgE_FL) Rg histogram

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W

R_g^Guinier	3.7	nm
D_max	18.1	nm
Volume^Porod	102	nm³

SASDBT6 – GppNHp bound form of full length ObgE from E.coli (ObgE_FL with GppNHp)

UniProt ID: P42641 (1-390) GTPase ObgE/CgtA

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

R_g^Guinier	3.7	nm
D_max	14.5	nm
Volume^Porod	90	nm³

SASDBU6 – Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340)

UniProt ID: P42641 (1-340) GTPase ObgE/CgtA

Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Rg histogram

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Mar 12

R_g^Guinier	3.1	nm
D_max	11.6	nm
Volume^Porod	70	nm³

SASDBV6 – GppNHp bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GppNHp)

UniProt ID: P42641 (1-340) GTPase ObgE/CgtA

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Mar 12

R_g^Guinier	3.1	nm
D_max	11.4	nm
Volume^Porod	72	nm³

SASDBW6 – Suppressor of Copper Sensitivity C protein (ScsC) from P. mirabilis (mutant)

UniProt ID: C2LPE2 (22-243) Suppressor of Copper Sensitivity C protein (mutant)

Suppressor of Copper Sensitivity C protein (mutant) experimental SAS data

Suppressor of Copper Sensitivity C protein (ScsC) from P. mirabilis (mutant) Rg histogram

Sample:	Suppressor of Copper Sensitivity C protein (mutant) trimer, 73 kDa Proteus mirabilis protein
Buffer:	25 mM HEPES 150mM NaCl, 1mM DTT,, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Jul 22

A shape-shifting redox foldase contributes to Proteus mirabilis copper resistance. Nat Commun 8:16065 (2017)
Furlong EJ, Lo AW, Kurth F, Premkumar L, Totsika M, Achard MES, Halili MA, Heras B, Whitten AE, Choudhury HG, Schembri MA, Martin JL

R_g^Guinier	4.4	nm
D_max	13.5	nm
Volume^Porod	108	nm³

SASDBZ6 – Draxin

UniProt ID: Q8NBI3 (None-None) Draxin

Sample:	Draxin monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19

Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R

R_g^Guinier	4.2	nm
D_max	15.0	nm
Volume^Porod	87	nm³

SASDB47 – Full length tetrameric FKBP39 protein from Drosophila melanogaster

UniProt ID: P54397 (1-357) 39 kDa FK506-binding nuclear protein

39 kDa FK506-binding nuclear protein experimental SAS data

Full length tetrameric FKBP39 protein from Drosophila melanogaster Rg histogram

Sample:	39 kDa FK506-binding nuclear protein tetramer, 163 kDa Drosophila melanogaster protein
Buffer:	50 mM Na2HPO4 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2014 Nov 14

Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments. Sci Rep 7:40405 (2017)
Kozłowska M, Tarczewska A, Jakób M, Bystranowska D, Taube M, Kozak M, Czarnocki-Cieciura M, Dziembowski A, Orłowski M, Tkocz K, Ożyhar A

R_g^Guinier	5.7	nm
D_max	22.0	nm
Volume^Porod	275	nm³

SASDBE7 – ESX-5 type VII secretion system protein EccC5

UniProt ID: I0RZI0 (None-None) ESX-5 type VII secretion system protein EccC5

ESX-5 type VII secretion system protein EccC5 experimental SAS data

ESX-5 type VII secretion system protein EccC5 Rg histogram

Sample:	ESX-5 type VII secretion system protein EccC5 monomer, 142 kDa Mycobacterium xenopi RIVM700367 protein
Buffer:	20 mM Tris 200 mM NaCl 5%(v/v) Glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jul 15

Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis. Nat Microbiol 2:17047 (2017)
Beckham KS, Ciccarelli L, Bunduc CM, Mertens HD, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski MM, Svergun DI, Bitter W, Wilmanns M, Marlovits TC, Parret AH, Houben EN

R_g^Guinier	6.2	nm
D_max	23.0	nm
Volume^Porod	255	nm³

SASDBK7 – Vaccinia virus A46 protein (full-length)

UniProt ID: P26672 (None-None) Protein A46

Sample:	Protein A46 tetramer, 112 kDa Vaccinia virus protein
Buffer:	20 mM Tris-HCl, 10 mM DTT, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2015 Jun 25

Vaccinia Virus Immunomodulator A46: A Lipid and Protein-Binding Scaffold for Sequestering Host TIR-Domain Proteins. PLoS Pathog 12(12):e1006079 (2016)
Fedosyuk S, Bezerra GA, Radakovics K, Smith TK, Sammito M, Bobik N, Round A, Ten Eyck LF, Djinović-Carugo K, Usón I, Skern T

R_g^Guinier	4.3	nm
D_max	14.0	nm
Volume^Porod	199	nm³

SASDBL7 – N-terminal domain of Vaccinia virus A46 protein (1-83)

UniProt ID: P26672 (1-83) Protein A46

Sample:	Protein A46 tetramer, 40 kDa Vaccinia virus protein
Buffer:	20 mM Tris-HCl, 10 mM DTT, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2015 Jun 25

R_g^Guinier	2.6	nm
D_max	9.0	nm
Volume^Porod	68	nm³

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