SASBDB entries for UniProt ID:

SASDBC6 – Compound 6:BRD4 (2:1)

UniProt ID: O60885 (1-477) Bromodomain-containing protein 4
Bromodomain-containing protein 4 experimental SAS data
DAMMIF model
Sample: Bromodomain-containing protein 4 monomer, 56 kDa Homo sapiens protein
Buffer: 20mM Hepes, 100mM NaCl, 1mM Tris(2-carboxyethyl)phosphine hydrochloride, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 8
Potent and selective bivalent inhibitors of BET bromodomains. Nat Chem Biol 12(12):1097-1104 (2016)
Waring MJ, Chen H, Rabow AA, Walker G, Bobby R, Boiko S, Bradbury RH, Callis R, Clark E, Dale I, Daniels DL, Dulak A, Flavell L, Holdgate G, Jowitt TA, Kikhney A, McAlister M, Méndez J, Ogg D, Patel J, Petteruti P, Robb GR, Robers MB, Saif S, Stratton N, Svergun DI, Wang W, Whittaker D, Wilson DM, Yao Y
RgGuinier 4.4 nm
Dmax 17.3 nm

SASDBG6 – Ribosome biogenesis protein 15 (Nop15)

UniProt ID: P53927 (81-220) Ribosome biogenesis protein 15
Ribosome biogenesis protein 15 experimental SAS data
EOM/RANCH model
Sample: Ribosome biogenesis protein 15 monomer, 17 kDa Saccharomyces cerevisiae protein
Buffer: 25 mM HEPES, 500 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Apr 25
Structural analysis reveals the flexible C-terminus of Nop15 undergoes rearrangement to recognize a pre-ribosomal RNA folding intermediate. Nucleic Acids Res 45(5):2829-2837 (2017)
Zhang J, Gonzalez LE, Hall TMT
RgGuinier 2.4 nm
Dmax 10.3 nm
VolumePorod 38 nm3

SASDBS6 – Apo form of full length ObgE from E.coli (ObgE_FL)

UniProt ID: P42641 (1-390) GTPase ObgE/CgtA
GTPase ObgE/CgtA experimental SAS data
Apo form of full length ObgE from E.coli (ObgE_FL) Rg histogram
Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.7 nm
Dmax 18.1 nm
VolumePorod 102 nm3

SASDBT6 – GppNHp bound form of full length ObgE from E.coli (ObgE_FL with GppNHp)

UniProt ID: P42641 (1-390) GTPase ObgE/CgtA
GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.7 nm
Dmax 14.5 nm
VolumePorod 90 nm3

SASDBU6 – Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340)

UniProt ID: P42641 (1-340) GTPase ObgE/CgtA
GTPase ObgE/CgtA experimental SAS data
Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Rg histogram
Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Mar 12
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.1 nm
Dmax 11.6 nm
VolumePorod 70 nm3

SASDBV6 – GppNHp bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GppNHp)

UniProt ID: P42641 (1-340) GTPase ObgE/CgtA
GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Mar 12
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.1 nm
Dmax 11.4 nm
VolumePorod 72 nm3

SASDBW6 – Suppressor of Copper Sensitivity C protein (ScsC) from P. mirabilis (mutant)

UniProt ID: C2LPE2 (22-243) Suppressor of Copper Sensitivity C protein (mutant)
Suppressor of Copper Sensitivity C protein (mutant) experimental SAS data
Suppressor of Copper Sensitivity C protein (ScsC) from P. mirabilis (mutant) Rg histogram
Sample: Suppressor of Copper Sensitivity C protein (mutant) trimer, 73 kDa Proteus mirabilis protein
Buffer: 25 mM HEPES 150mM NaCl, 1mM DTT,, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Jul 22
A shape-shifting redox foldase contributes to Proteus mirabilis copper resistance. Nat Commun 8:16065 (2017)
Furlong EJ, Lo AW, Kurth F, Premkumar L, Totsika M, Achard MES, Halili MA, Heras B, Whitten AE, Choudhury HG, Schembri MA, Martin JL
RgGuinier 4.4 nm
Dmax 13.5 nm
VolumePorod 108 nm3

SASDBZ6 – Draxin

UniProt ID: Q8NBI3 (None-None) Draxin
Draxin experimental SAS data
Draxin Rg histogram
Sample: Draxin monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 87 nm3

SASDB47 – Full length tetrameric FKBP39 protein from Drosophila melanogaster

UniProt ID: P54397 (1-357) 39 kDa FK506-binding nuclear protein
39 kDa FK506-binding nuclear protein experimental SAS data
Full length tetrameric FKBP39 protein from Drosophila melanogaster Rg histogram
Sample: 39 kDa FK506-binding nuclear protein tetramer, 163 kDa Drosophila melanogaster protein
Buffer: 50 mM Na2HPO4 150 mM NaCl, pH: 7
Experiment: SAXS data collected at B21, Diamond Light Source on 2014 Nov 14
Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments. Sci Rep 7:40405 (2017)
Kozłowska M, Tarczewska A, Jakób M, Bystranowska D, Taube M, Kozak M, Czarnocki-Cieciura M, Dziembowski A, Orłowski M, Tkocz K, Ożyhar A
RgGuinier 5.7 nm
Dmax 22.0 nm
VolumePorod 275 nm3

SASDBE7 – ESX-5 type VII secretion system protein EccC5

UniProt ID: I0RZI0 (None-None) ESX-5 type VII secretion system protein EccC5
ESX-5 type VII secretion system protein EccC5 experimental SAS data
ESX-5 type VII secretion system protein EccC5 Rg histogram
Sample: ESX-5 type VII secretion system protein EccC5 monomer, 142 kDa Mycobacterium xenopi RIVM700367 protein
Buffer: 20 mM Tris 200 mM NaCl 5%(v/v) Glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 15
Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis. Nat Microbiol 2:17047 (2017)
Beckham KS, Ciccarelli L, Bunduc CM, Mertens HD, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski MM, Svergun DI, Bitter W, Wilmanns M, Marlovits TC, Parret AH, Houben EN
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 255 nm3