SASBDB entries for UniProt ID:

SASDWT6 – Frataxin bound to tailored nanobody 16C10

UniProt ID: Q16595 (90-210) Frataxin, mitochondrial

UniProt ID: None (1-139) Nanobody 16C10
Frataxin, mitochondrialNanobody 16C10 experimental SAS data
COOT model
Sample: Frataxin, mitochondrial monomer, 14 kDa Homo sapiens protein
Nanobody 16C10 monomer, 15 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Sep 18
Nanobodies as tools for studying human frataxin biology. Commun Biol (2026)
Pignataro MF, Fernández NB, Garay-Alvarez A, Pavan MF, Molina R, Muñoz IG, Grossi J, Noguera M, Vila A, García AE, Gentili HG, Rodríguez NA, Aran M, Parreño V, Bok M, Hermoso JA, Ibañez LI, Santos J
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 37 nm3

SASDXB7 – Entamoeba histolytica Anti-Silencing Function 1 (ASF1) with C terminal His6-tag

UniProt ID: C4LX08 (1-234) Anti-Silencing Function 1
Anti-Silencing Function 1 experimental SAS data
ALPHAFOLD model
Sample: Anti-Silencing Function 1 monomer, 28 kDa Entamoeba histolytica strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Oct 1
Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone. Biochimie (2025)
Gandhi S, Vasudevan D
RgGuinier 3.5 nm
Dmax 10.1 nm
VolumePorod 40 nm3

SASDYV3 – Conjugative protein of the F plasmid Type IV Secretion System, TraW.

UniProt ID: P18472 (19-210) TraW (Δ1-18)
TraW (Δ1-18) experimental SAS data
DAMMIN model
Sample: TraW (Δ1-18) monomer, 26 kDa Escherichia coli (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 23
Solution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system Structural Dynamics 13(2) (2026)
Rodriguez C, Audette G
RgGuinier 2.6 nm
Dmax 9.0 nm

SASDYG4 – Yeast Elongation Factor like-1 protein (EFL1)

UniProt ID: P53893 (1-1110) Ribosome assembly protein 1 with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH)
Ribosome assembly protein 1 with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) experimental SAS data
Ribosome assembly protein 1 with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) Kratky plot
Sample: Ribosome assembly protein 1 with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) monomer, 127 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM HEPES, 300 mM NaCl, 5 mM MgCl2, 5% glycerol, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Oct 3
Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation. Protein Sci 35(4):e70504 (2026)
Zúñiga-Domínguez JA, Jain R, González-Andrade M, Farquhar ER, Chance MR, Gijsbers A, Sánchez-Puig N
RgGuinier 4.6 nm
Dmax 15.7 nm
VolumePorod 368 nm3

SASDAE6 – PsrP functional binding region

UniProt ID: A0A0H2URK1 (120-395) Functional binding region (120-395) of the pneumococcal serine-rich repeat protein
Functional binding region (120-395) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: Functional binding region (120-395) of the pneumococcal serine-rich repeat protein monomer, 30 kDa Streptococcus pneumoniae protein
Buffer: PBS 5 % Glycerol, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 27
The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016)
Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
RgGuinier 2.9 nm
Dmax 12.5 nm
VolumePorod 41 nm3

SASDA78 – Carbonic Anhydrase in Tris/HCl

UniProt ID: P00921 (None-None) Carbonic anhydrase 2
Carbonic anhydrase 2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: 100 mM Tris/HCl 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 19
Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering Journal of Applied Crystallography 40(s1):s245-s249 (2007)
Mylonas E, Svergun D
RgGuinier 2.1 nm
Dmax 6.0 nm

SASDA59 – slp-B53 with Ca2+

UniProt ID: M4N8T6 (None-None) S-layer protein
S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water with Ca2+, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
Liu J, Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 6.4 nm
Dmax 28.1 nm
VolumePorod 609 nm3

SASDAB9 – EcPaaA2-HisEcParE2 construct

UniProt ID: A0A0D7C2L1 (2-92) Plasmid stabilization protein ParE

UniProt ID: A0A0F6F6Q9 (14-75) Uncharacterized protein (Antitoxin)
Plasmid stabilization protein ParEUncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: Plasmid stabilization protein ParE octamer, 102 kDa Escherichia coli protein
Uncharacterized protein (Antitoxin) octamer, 68 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 3.3 nm
Dmax 15.3 nm
VolumePorod 166 nm3

SASDB26 – Glycosylated myelin-associated glycoprotein full extracellular domain (Ig1-5) I473E mutant

UniProt ID: P20917 (20-508) Myelin-associated glycoprotein (20-508; I473E mutant)
Myelin-associated glycoprotein (20-508; I473E mutant) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myelin-associated glycoprotein (20-508; I473E mutant) monomer, 54 kDa Mus musculus protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11
Structural basis of myelin-associated glycoprotein adhesion and signalling. Nat Commun 7:13584 (2016)
Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
RgGuinier 6.0 nm
Dmax 20.6 nm
VolumePorod 117 nm3

SASDBJ6 – Truncated construct of human p23 (1-142)

UniProt ID: Q15185 (1-142) Prostaglandin E synthase 3 (1-142)
Prostaglandin E synthase 3 (1-142) experimental SAS data
Prostaglandin E synthase 3 (1-142) Kratky plot
Sample: Prostaglandin E synthase 3 (1-142) monomer, 17 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22
The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
Seraphim TV, Gava LM, Mokry DZ, Cagliari TC, Barbosa LR, Ramos CH, Borges JC
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 36 nm3