SASBDB entries for UniProt ID:

SASDG66 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

UniProt ID: P62136 (1-330) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

UniProt ID: Q8WUF5 (621-828) Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)
Serine/threonine-protein phosphatase PP1-alpha catalytic subunitInhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 25
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
Zhou Y, Millott R, Kim HJ, Peng S, Edwards RA, Skene-Arnold T, Hammel M, Lees-Miller SP, Tainer JA, Holmes CFB, Glover JNM
RgGuinier 3.0 nm
Dmax 10.9 nm
VolumePorod 116 nm3

SASDH69 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated

UniProt ID: P11717 (1222-1510) Cation-independent mannose-6-phosphate receptor
Cation-independent mannose-6-phosphate receptor experimental SAS data
OTHER model
Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
Bochel A, Williams C, McCoy A, Hoppe H, Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 48 nm3

SASDHX9 – Phosphorylated phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): pDHPHDEP1

UniProt ID: Q8TCU6 (38-499) Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 experimental SAS data
Phosphorylated phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): pDHPHDEP1 Rg histogram
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity andcontributes to membrane binding. J Biol Chem (2020)
Ravala SK, Hopkins JB, Plescia CB, Allgood SR, Kane MA, Cash JN, Stahelin RV, Tesmer JJG
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 75 nm3

SASDJP2 – Truncated tyrosyl-DNA phosphodiesterase 1 (TDP1 149-608)

UniProt ID: Q9NUW8 (149-698) Tyrosyl-DNA phosphodiesterase 1 (149-608)
Tyrosyl-DNA phosphodiesterase 1 (149-608) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tyrosyl-DNA phosphodiesterase 1 (149-608) monomer, 53 kDa Homo sapiens protein
Buffer: 200 mM NaCl, 20 mM Tris-HCl, pH 7.5, 2% glycerol,, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 18
Direct interaction of DNA repair protein tyrosyl DNA phosphodiesterase 1 and the DNA ligase III catalytic domain is regulated by phosphorylation of its flexible N-terminus. J Biol Chem :100921 (2021)
Rashid I, Hammel M, Sverzhinsky A, Tsai MS, Pascal JM, Tainer JA, Tomkinson AE
RgGuinier 2.3 nm
Dmax 7.0 nm
VolumePorod 80 nm3

SASDJJ4 – Collagen VI von Willebrand factor (VWA) N2 domain

UniProt ID: P12111 (1634-1833) Collagen alpha-3(VI) chain, N2 domain
Collagen alpha-3(VI) chain, N2 domain experimental SAS data
DAMMIN model
Sample: Collagen alpha-3(VI) chain, N2 domain monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM TRIS, pH 7.4, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 4
Structure of a collagen VI α3 chain VWA domain array: adaptability and functional implications of myopathy causing mutations Journal of Biological Chemistry :jbc.RA120.014865 (2020)
Solomon-Degefa H, Gebauer J, Jeffries C, Freiburg C, Meckelburg P, Bird L, Baumann U, Svergun D, Owens R, Werner J, Behrmann E, Paulsson M, Wagener R
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 40 nm3

SASDJQ4 – Mitochondrial import inner membrane translocase subunits TIM8-TIM13

UniProt ID: P57744 (1-87) Mitochondrial import inner membrane translocase subunit TIM8

UniProt ID: P53299 (2-105) Mitochondrial import inner membrane translocase subunit TIM13
Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
Sučec I, Wang Y, Dakhlaoui O, Weinhäupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P
RgGuinier 3.1 nm
Dmax 10.9 nm
VolumePorod 136 nm3

SASDJW4 – X-ray repair cross-complementing proteins 5 and 6-DNA

UniProt ID: P12956 (1-609) X-ray repair cross-complementing protein 6

UniProt ID: P13010 (1-732) X-ray repair cross-complementing protein 5

UniProt ID: None (None-None) Y-DNA
X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5Y-DNA experimental SAS data
BILBOMD model
Sample: X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein
X-ray repair cross-complementing protein 5 monomer, 83 kDa Homo sapiens protein
Y-DNA monomer, 18 kDa DNA
Buffer: 50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Jan 8
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
Hammel M, Rosenberg DJ, Bierma J, Hura GL, Lees-Miller SP, Tainer JA
RgGuinier 4.1 nm
Dmax 14.8 nm
VolumePorod 280 nm3

SASDJD5 – LINC complex between the SUN domain of SUN1 and KASH domain of KASH5 - SUN1-KASH5 6:6 complex

UniProt ID: O94901 (616-812) SUN domain-containing protein 1

UniProt ID: Q8N6L0 (542-562) Protein KASH5
SUN domain-containing protein 1Protein KASH5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein
Protein KASH5 hexamer, 20 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 17
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 244 nm3

SASDJR5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 37°C

UniProt ID: Q9I589 (26-389) Chitin-binding protein CbpD
Chitin-binding protein CbpD experimental SAS data
Chitin-binding protein CbpD Kratky plot
Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar with InCoatec Cu microsource, RECX, University of Oslo on 2019 Aug 14
The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection. Nat Commun 12(1):1230 (2021)
Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lewis NE, Nizet V, Vaaje-Kolstad G
RgGuinier 3.5 nm
Dmax 15.0 nm
VolumePorod 63 nm3

SASDK22 – Mammalian cell entry protein 1A (Mce1A126-454)

UniProt ID: Q79FZ9 (126-454) Mce-family protein Mce1A

UniProt ID: None (None-None) n-Dodecyl-β-D-Maltopyranoside
Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mce-family protein Mce1A monomer, 39 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 123 kDa
Buffer: 50 mM Tris, 350 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Nov 28
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 5.5 nm
Dmax 22.0 nm
VolumePorod 216 nm3