SASBDB entries for UniProt ID:

SASDDG9 – The 2:1 complex of Synechocystis disulphide-trapped Fluorescence recovery protein dimer (CC mutant) and orange carotenoid-binding protein-ΔNTE (orange form)

UniProt ID: P74103 (None-None) Fluorescence recovery protein

UniProt ID: P74102 (None-None) Orange carotenoid-binding protein
Fluorescence recovery proteinOrange carotenoid-binding protein experimental SAS data
GASBOR model
Sample: Fluorescence recovery protein dimer, 28 kDa Synechocystis sp. PCC … protein
Orange carotenoid-binding protein monomer, 34 kDa Synechocystis sp. PCC … protein
Buffer: 20 mM Tris, 150 mM NaCl, 3% glycerol, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria. Nat Commun 9(1):3869 (2018)
Sluchanko NN, Slonimskiy YB, Shirshin EA, Moldenhauer M, Friedrich T, Maksimov EG
RgGuinier 3.0 nm
Dmax 13.0 nm
VolumePorod 102 nm3

SASDDH9 – Chloroplastic phosphoribulokinase (collected using SEC-SAXS)

UniProt ID: P19824 (32-375) Phosphoribulokinase, chloroplastic
Phosphoribulokinase, chloroplastic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Phosphoribulokinase, chloroplastic dimer, 78 kDa Chlamydomonas reinhardtii protein
Buffer: Tris-HCl 50 mM 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 15
Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin-Benson cycle. Proc Natl Acad Sci U S A 116(16):8048-8053 (2019)
Gurrieri L, Del Giudice A, Demitri N, Falini G, Pavel NV, Zaffagnini M, Polentarutti M, Crozet P, Marchand CH, Henri J, Trost P, Lemaire SD, Sparla F, Fermani S
RgGuinier 3.4 nm
Dmax 11.3 nm
VolumePorod 115 nm3

SASDDJ9 – Conformation of the R1-3 human dystrophin fragment (SANS)

UniProt ID: P11532 (337-668) R1-3 human dystrophin fragment
R1-3 human dystrophin fragment experimental SAS data
YASARA model
Sample: R1-3 human dystrophin fragment monomer, 39 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% D2O, pD 7.5, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 7
Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids. Biophys J 115(7):1231-1239 (2018)
Dos Santos Morais R, Delalande O, Pérez J, Mias-Lucquin D, Lagarrigue M, Martel A, Molza AE, Chéron A, Raguénès-Nicol C, Chenuel T, Bondon A, Appavou MS, Le Rumeur E, Combet S, Hubert JF
RgGuinier 4.2 nm
Dmax 17.7 nm
VolumePorod 46 nm3

SASDDK9 – Conformation of R1-3 human dystrophin fragment in interaction with zwitterionic phospholipid bicelles (SANS)

UniProt ID: P11532 (338-668) R1-3 human dystrophin fragment
R1-3 human dystrophin fragment experimental SAS data
DAMMIF model
Sample: R1-3 human dystrophin fragment monomer, 39 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% D2O, pD 7.5, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 7
Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids. Biophys J 115(7):1231-1239 (2018)
Dos Santos Morais R, Delalande O, Pérez J, Mias-Lucquin D, Lagarrigue M, Martel A, Molza AE, Chéron A, Raguénès-Nicol C, Chenuel T, Bondon A, Appavou MS, Le Rumeur E, Combet S, Hubert JF
RgGuinier 4.1 nm
Dmax 17.8 nm
VolumePorod 50 nm3

SASDDL9 – Conformation of R1-3 human dystrophin fragment in interaction with anionic phospholipid bicelles (SANS)

UniProt ID: P11532 (338-668) R1-3 human dystrophin fragment
R1-3 human dystrophin fragment experimental SAS data
YASARA model
Sample: R1-3 human dystrophin fragment monomer, 39 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% D2O, pD 7.5, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 7
Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids. Biophys J 115(7):1231-1239 (2018)
Dos Santos Morais R, Delalande O, Pérez J, Mias-Lucquin D, Lagarrigue M, Martel A, Molza AE, Chéron A, Raguénès-Nicol C, Chenuel T, Bondon A, Appavou MS, Le Rumeur E, Combet S, Hubert JF
RgGuinier 6.2 nm
Dmax 24.8 nm
VolumePorod 100 nm3

SASDDM9 – lipoprotein lipase–GPIHBP1 complex

UniProt ID: P06858 (29-475) Lipoprotein lipase

UniProt ID: None (None-None) Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
Lipoprotein lipaseGlycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 experimental SAS data
DAMMIF model
Sample: Lipoprotein lipase dimer, 101 kDa Homo sapiens protein
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 dimer, 29 kDa Homo sapiens protein
Buffer: 10 mM BisTris, 150 mM NaCl, 4 mM CaCl2, 10% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 2
Structure of the lipoprotein lipase-GPIHBP1 complex that mediates plasma triglyceride hydrolysis. Proc Natl Acad Sci U S A (2018)
Birrane G, Beigneux AP, Dwyer B, Strack-Logue B, Kristensen KK, Francone OL, Fong LG, Mertens HDT, Pan CQ, Ploug M, Young SG, Meiyappan M
RgGuinier 5.5 nm
Dmax 19.0 nm
VolumePorod 319 nm3

SASDDR9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer)

UniProt ID: Q8TDZ2 (None-None) [F-actin]-monooxygenase MICAL1 (monomer)
[F-actin]-monooxygenase MICAL1 (monomer) experimental SAS data
CORAL model
Sample: [F-actin]-monooxygenase MICAL1 (monomer) monomer, 118 kDa Homo sapiens protein
Buffer: 50 mM sodium phosphate buffer, pH 7.5, 5 % glycerol, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 20
Human MICAL1: activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8. Protein Sci (2018)
Esposito A, Ventura V, Petoukhov MV, Rai A, Svergun DI, Vanoni MA
RgGuinier 3.7 nm
Dmax 12.1 nm
VolumePorod 212 nm3

SASDDS9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) (Truncated MOCHLIM construct)

UniProt ID: Q8TDZ2 (1-778) [F-actin]-monooxygenase MICAL1 (MoChLim)
[F-actin]-monooxygenase MICAL1 (MoChLim) experimental SAS data
CORAL model
Sample: [F-actin]-monooxygenase MICAL1 (MoChLim) monomer, 85 kDa Homo sapiens protein
Buffer: 20 mM Hepes/NaOH, pH 7.5, 50 mM NaCl, 2 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 20
Human MICAL1: activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8. Protein Sci (2018)
Esposito A, Ventura V, Petoukhov MV, Rai A, Svergun DI, Vanoni MA
RgGuinier 4.0 nm
Dmax 18.0 nm
VolumePorod 145 nm3

SASDDT9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) in complex with Ras-related protein Rab 8 (MICAL1-Rab8 complex)

UniProt ID: Q8TDZ2 (None-None) [F-actin]-monooxygenase MICAL1 (monomer)

UniProt ID: P61026 (1-175) Ras-related protein 8
[F-actin]-monooxygenase MICAL1 (monomer)Ras-related protein 8 experimental SAS data
CORAL model
Sample: [F-actin]-monooxygenase MICAL1 (monomer) monomer, 118 kDa Homo sapiens protein
Ras-related protein 8 monomer, 20 kDa protein
Buffer: 20 mM Hepes/NaOH, pH 7.5, 50 mM NaCl, 2 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 20
Human MICAL1: activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8. Protein Sci (2018)
Esposito A, Ventura V, Petoukhov MV, Rai A, Svergun DI, Vanoni MA
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 234 nm3

SASDDU9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) (Truncated MOCH construct)

UniProt ID: Q8TDZ2 (1-614) [F-actin]-monooxygenase MICAL1 (MoCh)
[F-actin]-monooxygenase MICAL1 (MoCh) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: [F-actin]-monooxygenase MICAL1 (MoCh) monomer, 67 kDa Homo sapiens protein
Buffer: 50 mM sodium phosphate buffer, 5 % glycerol, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 6
Human MICAL1: activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8. Protein Sci (2018)
Esposito A, Ventura V, Petoukhov MV, Rai A, Svergun DI, Vanoni MA
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 100 nm3