Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDDW9 – PAS fold family protein mPAC-Δ132 in the dark state (mPAC-Δ132, dark, without adenosine-5'-[(α,β)-methyleno]triphosphate, ApCpp)

UniProt ID: B4VKN6 (133-483) PAS fold family

Sample:	PAS fold family dimer, 78 kDa Coleofasciculus chthonoplastes PCC … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 5 % w/v Glycerol, pH: 7.5
Experiment:	SAXS data collected at cSAXS, Swiss Light Source on 2015 Mar 11

MPAC Delta132
Robert Lindner

R_g^Guinier	4.4	nm
D_max	15.9	nm
Volume^Porod	132	nm³

SASDDX9 – PAS fold family protein mPAC-Δ132 with the addition of adenosine-5'-[(α,β)-methyleno]triphosphate in the dark state (mPAC-Δ132, dark + ApCpp)

UniProt ID: B4VKN6 (133-483) PAS fold family

Sample:	PAS fold family dimer, 78 kDa Coleofasciculus chthonoplastes PCC … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 5 % w/v Glycerol, 1 mM ApCpp, pH: 7.5
Experiment:	SAXS data collected at cSAXS, Swiss Light Source on 2015 Mar 11

MPAC Delta132
Robert Lindner

R_g^Guinier	4.0	nm
D_max	12.8	nm
Volume^Porod	99	nm³

SASDE32 – Mothers against decapentaplegic homolog 5 - Smad5 MH1

UniProt ID: Q99717 (9-138) Mothers against decapentaplegic homolog 5

Mothers against decapentaplegic homolog 5 experimental SAS data

Mothers against decapentaplegic homolog 5 Kratky plot

Sample:	Mothers against decapentaplegic homolog 5, 15 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 14

Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes (2019)
Ruiz L, Kaczmarska Z, Gomes T, Aragón E, Torner C, Freier R, Bagiński B, Martin-Malpartida P, de Martin Garrido N, Márquez J, Cordeiro T, Pluta R, Macias M

R_g^Guinier	1.9	nm
D_max	6.6	nm
Volume^Porod	32	nm³

SASDE42 – Mothers against decapentaplegic homolog 8_9 - Smad8_9 MH1

UniProt ID: O15198 (16-141) Mothers against decapentaplegic homolog 8_9

Mothers against decapentaplegic homolog 8_9 experimental SAS data

Mothers against decapentaplegic homolog 8_9 Kratky plot

Sample:	Mothers against decapentaplegic homolog 8_9, 15 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 1

R_g^Guinier	1.9	nm
D_max	6.5	nm
Volume^Porod	34	nm³

SASDE52 – Ribonuclease E from Escherichia coli

UniProt ID: P21513 (1-529) Endoribonuclease E

Endoribonuclease E experimental SAS data

Sample:	Endoribonuclease E tetramer, 247 kDa Escherichia coli protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ

R_g^Guinier	5.0	nm
D_max	16.1	nm
Volume^Porod	468	nm³

SASDE62 – Ribonuclease E from Yersinia pestis

UniProt ID: Q74TC3 (1-529) Endoribonuclease E

Sample:	Endoribonuclease E tetramer, 248 kDa Yersinia pestis protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	5.1	nm
D_max	16.4	nm
Volume^Porod	470	nm³

SASDE72 – Ribonuclease E from Francisella tularensis

UniProt ID: Q5NFK7 (1-543) Endoribonuclease E

Sample:	Endoribonuclease E tetramer, 256 kDa Francisella tularensis protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	5.1	nm
D_max	17.2	nm
Volume^Porod	491	nm³

SASDE82 – Ribonuclease E from Burkholderia pseudomallei

UniProt ID: A0A0H3HN63 (1-532) Endoribonuclease E

Sample:	Endoribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	4.8	nm
D_max	14.9	nm
Volume^Porod	437	nm³

SASDE92 – Ribonuclease E from Acinetobacter baumannii

UniProt ID: A0A0B9WR03 (1-544) Endoribonuclease E

Sample:	Endoribonuclease E tetramer, 254 kDa Acinetobacter baumannii protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	5.2	nm
D_max	18.3	nm
Volume^Porod	508	nm³

SASDEB2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73E

UniProt ID: P9WMU1 (None-None) Cell wall synthesis protein Wag31

Cell wall synthesis protein Wag31 experimental SAS data

Cell wall synthesis protein Wag31 Kratky plot

Sample:	Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein
Buffer:	50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Dec 15

Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B

R_g^Guinier	19.8	nm
D_max	23.4	nm

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