SASBDB entries for UniProt ID:

SASDEK7 – SpaO C-terminus

UniProt ID: P40699 (140-297) Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus experimental SAS data
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus Kratky plot
Sample: Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus monomer, 19 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 2.1 nm
Dmax 7.2 nm

SASDEL7 – Human glycine decarboxylase

UniProt ID: P23378 (45-1020) Glycine decarboxylase
Glycine decarboxylase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 3.9 nm
VolumePorod 304 nm3

SASDEM7 – Human glycine decarboxylase with glycine

UniProt ID: P23378 (45-1020) Glycine decarboxylase
Glycine decarboxylase experimental SAS data
Glycine decarboxylase Kratky plot
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT , 100 mM glycine, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 4.0 nm
VolumePorod 315 nm3

SASDEW7 – C-terminal truncated bank vole prion protein (amino acids 90-231)

UniProt ID: Q8VHV5 (90-231) Major prion protein
Major prion protein experimental SAS data
C-terminal truncated bank vole prion protein (amino acids 90-231) Rg histogram
Sample: Major prion protein monomer, 16 kDa Myodes glareolus protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 25
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.3 nm
Dmax 9.4 nm
VolumePorod 29 nm3

SASDEX7 – C-terminal truncated bank vole prion protein (amino acids 90-231) bound to Cu(II)

UniProt ID: Q8VHV5 (90-231) Major prion protein
Major prion protein experimental SAS data
C-terminal truncated bank vole prion protein (amino acids 90-231) bound to Cu(II) Rg histogram
Sample: Major prion protein monomer, 16 kDa Myodes glareolus protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 25
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.2 nm
Dmax 8.7 nm
VolumePorod 30 nm3

SASDEY7 – C-terminal truncated ovine prion protein ARR (amino acids 94-234)

UniProt ID: P23907 (94-234) Major prion protein
Major prion protein experimental SAS data
C-terminal truncated ovine prion protein ARR (amino acids 94-234) Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.2 nm
Dmax 9.0 nm
VolumePorod 29 nm3

SASDEZ7 – C-terminal truncated ovine prion protein (94-234) with ARR polymorphism + Cu(II)

UniProt ID: P23907 (94-234) Major prion protein
Major prion protein experimental SAS data
C-terminal truncated ovine prion protein (94-234) with ARR polymorphism + Cu(II) Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.2 nm
Dmax 8.8 nm
VolumePorod 31 nm3

SASDE28 – C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism

UniProt ID: P23907 (94-234) Major prion protein
Major prion protein experimental SAS data
C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.1 nm
Dmax 9.1 nm
VolumePorod 29 nm3

SASDE38 – C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism + Cu(II)

UniProt ID: P23907 (94-234) Major prion protein
Major prion protein experimental SAS data
C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism + Cu(II) Rg histogram
Sample: Major prion protein monomer, 16 kDa Ovis aries protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 4
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.2 nm
Dmax 8.7 nm
VolumePorod 29 nm3

SASDE48 – Toxin GraT

UniProt ID: Q88MI5 (1-91) Putative killer protein, Toxin GraT.
Putative killer protein, Toxin GraT. experimental SAS data
Putative killer protein, Toxin GraT. Kratky plot
Sample: Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein
Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Sep 21
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
RgGuinier 1.5 nm
Dmax 5.5 nm
VolumePorod 18 nm3