SASBDB entries for UniProt ID:

SASDFZ4 – R16-24 human dystrophin fragment

UniProt ID: P11532 (1990-3040) Human dystrophin central domain R16-24 fragment
Human dystrophin central domain R16-24 fragment experimental SAS data
Human dystrophin central domain R16-24 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 fragment monomer, 127 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Dec 17
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 9.3 nm
Dmax 34.0 nm

SASDF25 – R16-24 del45-55 human dystrophin fragment

UniProt ID: P11532 (1990-3040) Human dystrophin central domain R16-24 del45-55 fragment
Human dystrophin central domain R16-24 del45-55 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-55 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-55 fragment monomer, 58 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Sep 28
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 4.7 nm
Dmax 18.3 nm

SASDF35 – R16-24 del45-51 human dystrophin fragment

UniProt ID: P11532 (1990-3040) Human dystrophin central domain R16-24 del45-51 fragment
Human dystrophin central domain R16-24 del45-51 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-51 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-51 fragment monomer, 85 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Jul 13
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 7.0 nm
Dmax 26.2 nm

SASDF45 – Leukocidin/Integrin alpha-M complex (LukGH-huCD11b-I) in the presence of a neutralizing antibody Fab fragment

UniProt ID: P21224 (30-338) Leukocidin G

UniProt ID: Q5HEH9 (28-351) Leukocidin H

UniProt ID: None (None-None) LukGH neutralizing antibody

UniProt ID: P11215 (143-337) Integrin alpha-M
Leukocidin GLeukocidin HLukGH neutralizing antibodyIntegrin alpha-M experimental SAS data
PYMOL model
Sample: Leukocidin G monomer, 36 kDa Staphylococcus aureus protein
Leukocidin H monomer, 38 kDa Staphylococcus aureus protein
LukGH neutralizing antibody monomer, 47 kDa Homo sapiens protein
Integrin alpha-M monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 300 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 17
Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity. Proc Natl Acad Sci U S A (2019)
Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A
RgGuinier 5.0 nm
Dmax 18.0 nm
VolumePorod 178 nm3

SASDF55 – Leukocidin (LukGH) from Staphylococcus aureus in complex with a neutralizing antibody

UniProt ID: P21224 (30-338) Leukocidin G

UniProt ID: Q5HEH9 (28-351) Leukocidin H

UniProt ID: None (None-None) LukGH neutralizing antibody
Leukocidin GLeukocidin HLukGH neutralizing antibody experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Leukocidin G monomer, 36 kDa Staphylococcus aureus protein
Leukocidin H monomer, 38 kDa Staphylococcus aureus protein
LukGH neutralizing antibody monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 300 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 17
Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity. Proc Natl Acad Sci U S A (2019)
Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A
RgGuinier 4.7 nm
Dmax 16.0 nm
VolumePorod 152 nm3

SASDF65 – Resistance to inhibitors of cholinesterase 8 homolog A (Ric8a) amino acids 1-492

UniProt ID: Q5E9J8 (1-492) Resistance to inhibitors of cholinesterase 8 homolog A
Resistance to inhibitors of cholinesterase 8 homolog A experimental SAS data
BILBOMD model
Sample: Resistance to inhibitors of cholinesterase 8 homolog A monomer, 56 kDa Bos taurus protein
Buffer: 20 mM Tris, 150 mM KCl, 5 % glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Oct 27
Structural underpinnings of Ric8A function as a G-protein α-subunit chaperone and guanine-nucleotide exchange factor. Nat Commun 10(1):3084 (2019)
Srivastava D, Gakhar L, Artemyev NO
RgGuinier 3.4 nm
Dmax 12.7 nm
VolumePorod 88 nm3

SASDF75 – Resistance to inhibitors of cholinesterase 8 homolog A (Ric8a) amino acids 1-452

UniProt ID: Q5E9J8 (1-452) Resistance to inhibitors of cholinesterase 8 homolog A
Resistance to inhibitors of cholinesterase 8 homolog A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Resistance to inhibitors of cholinesterase 8 homolog A monomer, 51 kDa Bos taurus protein
Buffer: 20 mM Tris, 150 mM KCl, 5 % glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Oct 27
Structural underpinnings of Ric8A function as a G-protein α-subunit chaperone and guanine-nucleotide exchange factor. Nat Commun 10(1):3084 (2019)
Srivastava D, Gakhar L, Artemyev NO
RgGuinier 3.0 nm
Dmax 11.2 nm
VolumePorod 70 nm3

SASDF85 – Pseudomonas aeruginosa antitoxin HigA: apo PaHigA

UniProt ID: Q9HVC1 (None-None) Uncharacterized protein
Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 21
Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 23 nm3

SASDF95 – Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex

UniProt ID: Q9HVC1 (None-None) Uncharacterized protein

UniProt ID: None (None-None) DNA Duplex
Uncharacterized proteinDNA Duplex experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
DNA Duplex dimer, 20 kDa DNA
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Sep 19
Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 81 nm3

SASDFA5 – Unposphorylated Resistance to inhibitors of cholinesterase 8 homolog A, Ric-8A, amino acids 1-452 (Rattus norvegicus)

UniProt ID: B1H241 (1-452) Resistance to inhibitors of cholinesterase 8 homolog A
Resistance to inhibitors of cholinesterase 8 homolog A experimental SAS data
Resistance to inhibitors of cholinesterase 8 homolog A Kratky plot
Sample: Resistance to inhibitors of cholinesterase 8 homolog A monomer, 51 kDa Rattus norvegicus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Apr 24
Structure, Function, and Dynamics of the Gα Binding Domain of Ric-8A. Structure (2019)
Zeng B, Mou TC, Doukov TI, Steiner A, Yu W, Papasergi-Scott M, Tall GG, Hagn F, Sprang SR
RgGuinier 3.0 nm
Dmax 10.6 nm
VolumePorod 70 nm3