Browse by MODEL: Hybrid

SASDKR3 – Deuterated calmodulin bound to the HIV-1 MA protein

Calmodulin-1calcium ionsGag-Pol polyprotein experimental SAS data
MONSA model
Sample: Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Calcium ions tetramer, 0 kDa
Gag-Pol polyprotein monomer, 15 kDa Human immunodeficiency virus … protein
Buffer: 50 mM MOPS, 5 mM CaCl2, 2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2010 Jun 23
Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. Biophys J 103(3):541-549 (2012)
Taylor JE, Chow JYH, Jeffries CM, Kwan AH, Duff AP, Hamilton WA, Trewhella J
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 55 nm3

SASDAE5 – CYNEX4

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) experimental SAS data
DAMMIF model
Sample: CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) monomer, 93 kDa Homo sapiens protein
Buffer: 50 mM HEPES 150 mM NaCl 1 mM EGTA, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Sep 11
Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI
RgGuinier 3.7 nm
Dmax 12.8 nm
VolumePorod 135 nm3

SASDAF5 – CYNEX4-T266D

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant experimental SAS data
DAMMIF model
Sample: CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant monomer, 93 kDa Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Sep 11
Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 146 nm3

SASDAJ5 – Annexin-A4

Annexin-A4 experimental SAS data
CRYSOL model
Sample: Annexin-A4 monomer, 36 kDa Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Sep 11
Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI
RgGuinier 2.4 nm
Dmax 7.6 nm
VolumePorod 54 nm3

SASDM97 – Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv)

Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv) experimental SAS data
BUNCH model
Sample: Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv) monomer, 29 kDa protein
Buffer: phosphate buffered saline, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Oct 21
Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment. Arch Biochem Biophys 522(1):26-36 (2012)
Paoletti F, Malerba F, Konarev PV, Visintin M, Scardigli R, Fasulo L, Lamba D, Svergun DI, Cattaneo A
RgGuinier 2.7 nm
Dmax 8.0 nm
VolumePorod 39 nm3

SASDDK2 – Aspergillus fumigatus UDP galactopyranose mutase

Aspergillus fumigatus UDP galactopyranose mutase experimental SAS data
MES-FOXS model
Sample: Aspergillus fumigatus UDP galactopyranose mutase tetramer, 228 kDa protein
Buffer: 20 mM HEPES, 45 mM NaCl, 0.5 mM Tris(hydroxypropyl)phosphine, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 19
Crystal structures and small-angle x-ray scattering analysis of UDP-galactopyranose mutase from the pathogenic fungus Aspergillus fumigatus. J Biol Chem 287(12):9041-51 (2012)
Dhatwalia R, Singh H, Oppenheimer M, Karr DB, Nix JC, Sobrado P, Tanner JJ
RgGuinier 4.7 nm
Dmax 14.7 nm
VolumePorod 308 nm3

SASDMJ9 – Plasmid pET11d- 229E710 encoding C-terminally His6-tagged HCoV-229E Nsp7-10 polyprotein

Replicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
RgGuinier 2.1 nm

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
RgGuinier 3.0 nm

SASDLG3 – Thermoplasma E2 catalytic core

Regulatory protein E2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Regulatory protein E2, 1037 kDa Human papillomavirus type … protein
Buffer: 50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Jul 13
The catalytic core of an archaeal 2-oxoacid dehydrogenase multienzyme complex is a 42-mer protein assembly FEBS Journal 279(5):713-723 (2012)
Marrott N, Marshall J, Svergun D, Crennell S, Hough D, Danson M, van den Elsen J
RgGuinier 8.8 nm
Dmax 22.0 nm
VolumePorod 2473 nm3

SASDAA4 – Full length GbpA

Full length GbpA experimental SAS data
GASBOR model
Sample: Full length GbpA monomer, 54 kDa Vibrio cholerae protein
Buffer: 25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 16
The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog 8(1):e1002373 (2012)
Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, Ibrahim AF, Svergun DI, Eijsink VG, Chatterjee NS, van Aalten DM
RgGuinier 3.9 nm
Dmax 14.5 nm
VolumePorod 100 nm3