Browse by MODEL: Hybrid

SASDJC5 – LINC complex between the SUN domain of SUN1 and KASH domain of Nesprin-4 - SUN1-KASH4 6:6 complex

SUN domain-containing protein 1Nesprin-4 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein
Nesprin-4 hexamer, 20 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 21
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 240 nm3

SASDJD5 – LINC complex between the SUN domain of SUN1 and KASH domain of KASH5 - SUN1-KASH5 6:6 complex

SUN domain-containing protein 1Protein KASH5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein
Protein KASH5 hexamer, 20 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 17
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 244 nm3

SASDJE5 – LINC complex between the SUN domain of SUN1 and KASH domain of Nesprin-1 - SUN1-KASH1 6:6 complex

SUN domain-containing protein 1Nesprin-1 experimental SAS data
CORAL model
Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein
Nesprin-1 hexamer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 17
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 3.9 nm
Dmax 13.0 nm
VolumePorod 254 nm3

SASDJF5 – SUN domain of SUN1 harbouring mutation I673E - monomer

SUN domain-containing protein 1, I673E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SUN domain-containing protein 1, I673E monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jul 29
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 2.1 nm
Dmax 8.2 nm
VolumePorod 42 nm3

SASDJ66 – GATA double strand DNA

24-mer double strand DNA from the GATA promoter experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 10 nm3

SASDJP5 – Beltless isolated translocation domain of tetanus nerotoxin (TeNT/blHn)

Tetanus toxin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tetanus toxin monomer, 35 kDa Clostridium tetani protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL45XU, SPring-8 on 2018 Oct 20
Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses. J Struct Biol X 5:100045 (2021)
Zhang CM, Imoto Y, Hikima T, Inoue T
RgGuinier 2.9 nm
Dmax 11.3 nm
VolumePorod 42 nm3

SASDJN8 – L19-IL2 dimeric immunocytokine

L19-IL2 dimeric immunocytokine experimental SAS data
ALLOSMOD model
Sample: L19-IL2 dimeric immunocytokine dimer, 84 kDa synthetic construct protein
Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 23
Inference of molecular structure for characterization and improvement of clinical grade immunocytokines Journal of Structural Biology :107696 (2021)
Ongaro T, Guarino S, Scietti L, Palamini M, Wulhfard S, Neri D, Villa A, Forneris F
RgGuinier 4.1 nm
Dmax 11.9 nm
VolumePorod 128 nm3

SASDJP8 – L19L19-IL2 immunocytokine monomer

L19L19-IL2 immunocytokine experimental SAS data
GASBOR model
Sample: L19L19-IL2 immunocytokine monomer, 69 kDa synthetic construct protein
Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 4
Inference of molecular structure for characterization and improvement of clinical grade immunocytokines Journal of Structural Biology :107696 (2021)
Ongaro T, Guarino S, Scietti L, Palamini M, Wulhfard S, Neri D, Villa A, Forneris F
RgGuinier 3.6 nm
Dmax 10.8 nm
VolumePorod 93 nm3

SASDJQ8 – IL12-L19L19 immunocytokine monomer

IL12-L19L19 immunocytokine experimental SAS data
GASBOR model
Sample: IL12-L19L19 immunocytokine monomer, 112 kDa synthetic construct protein
Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 4
Inference of molecular structure for characterization and improvement of clinical grade immunocytokines Journal of Structural Biology :107696 (2021)
Ongaro T, Guarino S, Scietti L, Palamini M, Wulhfard S, Neri D, Villa A, Forneris F
RgGuinier 4.5 nm
Dmax 14.2 nm
VolumePorod 224 nm3

SASDKQ4 – EspK secretion protein - full length

ESX-1 secretion-associated protein EspK experimental SAS data
CORAL model
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl pH, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 20
Structural Analysis of the Partially Disordered Protein EspK from Mycobacterium Tuberculosis Crystals 11(1):18 (2020)
Gijsbers A, Sánchez-Puig N, Gao Y, Peters P, Ravelli R, Siliqi D
RgGuinier 7.2 nm
Dmax 3.3 nm
VolumePorod 330 nm3