Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: No model

SASDMT8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 41% D2O

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data

Sample:	Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3

Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME

R_g^Guinier	2.2	nm
D_max	6.6	nm

SASDMU8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 86% D2O

Sample:	Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3

Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME

R_g^Guinier	3.1	nm
D_max	12.4	nm

SASDMV8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 100% D2O

Sample:	Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3

Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME

R_g^Guinier	4.0	nm
D_max	13.9	nm

SASDL39 – Autophagy-related protein 23

Autophagy-related protein 23 experimental SAS data

Autophagy-related protein 23 Kratky plot

Sample:	Autophagy-related protein 23 dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 3

Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ

R_g^Guinier	7.4	nm
D_max	29.3	nm
Volume^Porod	378	nm³

SASDL49 – Autophagy-related protein 23 L171A,I182A,L189A mutant

Autophagy-related protein 23 LIL Mutant experimental SAS data

Autophagy-related protein 23 LIL Mutant Kratky plot

Sample:	Autophagy-related protein 23 LIL Mutant monomer, 52 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8

Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ

R_g^Guinier	4.4	nm
D_max	17.1	nm
Volume^Porod	98	nm³

SASDL59 – Autophagy-related protein 23 R190A,K193A,K194A mutant

Autophagy-related protein 23 RKK Mutant experimental SAS data

Autophagy-related protein 23 RKK Mutant Kratky plot

Sample:	Autophagy-related protein 23 RKK Mutant dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8

Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ

R_g^Guinier	7.6	nm
D_max	34.5	nm
Volume^Porod	365	nm³

SASDNH3 – HIV-1 reverse transcriptase L289K mutation at 1 mg/mL

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) experimental SAS data

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Kratky plot

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	10.7	nm
Volume^Porod	91	nm³

SASDNJ3 – HIV-1 reverse transcriptase L289K mutation at 2 mg/mL

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	10.8	nm
Volume^Porod	91	nm³

SASDNK3 – HIV-1 reverse transcriptase L289K mutation at 4.3 mg/mL

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	10.7	nm
Volume^Porod	93	nm³

SASDMX5 – Zea mays beta-amylase (ZmBAM7) short

Sample:	Beta-amylase tetramer, 231 kDa Zea mays protein
Buffer:	50 mM HEPES, 25 mM NaCl, and 0.2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 7

The BAM7 gene in Zea mays encodes a protein with similar structural and catalytic properties to Arabidopsis BAM2 Acta Crystallographica Section D Structural Biology 78(5) (2022)
Ravenburg C, Riney M, Monroe J, Berndsen C

R_g^Guinier	5.3	nm
D_max	16.3	nm
Volume^Porod	615	nm³

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