Browse by MODEL: No model

SASDFP4 – Pro-matrix metalloproteinase-1 (MMP-1)

Pro-matrix metalloproteinase-1 (Interstitial collagenase) experimental SAS data
Pro-matrix metalloproteinase-1 (Interstitial collagenase) Kratky plot
Sample: Pro-matrix metalloproteinase-1 (Interstitial collagenase) monomer, 52 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 May 21
Conformationally constrained mutant of human matrix metalloproteinase-1
Rob Holland
RgGuinier 2.7 nm
Dmax 9.0 nm
VolumePorod 73 nm3

SASDFQ4 – Domain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1 S243C, S318C)

Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) experimental SAS data
Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) Kratky plot
Sample: Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) monomer, 52 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 May 21
Conformationally constrained mutant of human matrix metalloproteinase-1
Rob Holland
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 61 nm3

SASDCR8 – Glucose isomerase

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 100 mM HEPES, 1 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.7 nm

SASDCS8 – Cytochrome C

Cytochrome C experimental SAS data
Cytochrome C Kratky plot
Sample: Cytochrome C monomer, 12 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 1.3 nm

SASDDW9 – PAS fold family protein mPAC-Δ132 in the dark state (mPAC-Δ132, dark, without adenosine-5'-[(α,β)-methyleno]triphosphate, ApCpp)

PAS fold family experimental SAS data
PAS fold family Kratky plot
Sample: PAS fold family dimer, 78 kDa Coleofasciculus chthonoplastes PCC … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 5 % w/v Glycerol, pH: 7.5
Experiment: SAXS data collected at cSAXS, Swiss Light Source on 2015 Mar 11
MPAC Delta132
Robert Lindner
RgGuinier 4.4 nm
Dmax 15.9 nm
VolumePorod 132 nm3

SASDEM7 – Human glycine decarboxylase with glycine

Glycine decarboxylase experimental SAS data
Glycine decarboxylase Kratky plot
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT , 100 mM glycine, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 4.0 nm
VolumePorod 315 nm3

SASDEN7 – Neanderthal glycine decarboxylase

Glycine decarboxylase experimental SAS data
Glycine decarboxylase Kratky plot
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 4.0 nm
VolumePorod 309 nm3

SASDL53 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with continuous-flow SEC-SANS

Proton-gated ion channel experimental SAS data
Proton-gated ion channel Kratky plot
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 20
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 12.0 nm
VolumePorod 235 nm3

SASDL63 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with cuvette-mode SANS

Proton-gated ion channel experimental SAS data
Proton-gated ion channel Kratky plot
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 21
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 4.0 nm
Dmax 17.7 nm
VolumePorod 225 nm3

SASDKR7 – Hunchback mRNA translation repression complex of Brat-NHL, Pum-HD, Nanos-ZnF and hb NRE2 RNA

Brain tumor proteinMaternal protein pumilioProtein nanoshunchback mRNA Nanos Response Element 2 experimental SAS data
Brain tumor protein Maternal protein pumilio Protein nanos hunchback mRNA Nanos Response Element 2 Kratky plot
Sample: Brain tumor protein monomer, 32 kDa Drosophila melanogaster protein
Maternal protein pumilio monomer, 38 kDa Drosophila melanogaster protein
Protein nanos monomer, 11 kDa Drosophila melanogaster protein
Hunchback mRNA Nanos Response Element 2 monomer, 7 kDa Drosophila melanogaster RNA
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12
Structure and dynamics of the quaternary hunchback mRNA translation repression complex. Nucleic Acids Res 49(15):8866-8885 (2021)
Macošek J, Simon B, Linse JB, Jagtap PKA, Winter SL, Foot J, Lapouge K, Perez K, Rettel M, Ivanović MT, Masiewicz P, Murciano B, Savitski MM, Loedige I, Hub JS, Gabel F, Hennig J
RgGuinier 3.7 nm
Dmax 12.7 nm
VolumePorod 114 nm3