Small Angle Scattering Biological Data Bank SASBDB

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SASDL95 – Drosophila Melanogaster Gemin2-SMN: Octamer analyzed by SVD-EFA SEC-SAXS

Protein Gemin2Survival motor neuron protein experimental SAS data

Protein Gemin2 Survival motor neuron protein Kratky plot

Sample:	Protein Gemin2 octamer, 230 kDa Drosophila melanogaster protein Survival motor neuron protein octamer, 197 kDa Drosophila melanogaster protein
Buffer:	20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19

Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG

R_g^Guinier	7.8	nm
D_max	28.7	nm
Volume^Porod	2660	nm³

SASDK36 – LC-18t - ssDNA aptamer specific to lung adenocarcinoma cancer cells

Lung adenocarcinoma aptamer, truncated version experimental SAS data

Sample:	Lung adenocarcinoma aptamer, truncated version monomer, 11 kDa Artificially synthesized DNA
Buffer:	Phosphate-buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Oct 28

The Role of Small-Angle X-Ray Scattering and Molecular Simulations in 3D Structure Elucidation of a DNA Aptamer Against Lung Cancer Molecular Therapy - Nucleic Acids (2021)
Morozov D, Mironov V, Moryachkov R, Shchugoreva I, Artyushenko P, Zamay G, Kolovskaya O, Zamay T, Krat A, Molodenskiy D, Zabluda V, Veprintsev D, Sokolov A, Zukov R, Berezovski M, Tomilin F, Fedorov D, Alexeev Y, Kichkailo A

R_g^Guinier	2.1	nm
D_max	6.7	nm
Volume^Porod	16	nm³

SASDKX6 – PSK, an antimicrobial peptide from Chrysomya megacephala

PSK, an antimicrobial peptide from Chrysomya megacephala experimental SAS data

Sample:	PSK, an antimicrobial peptide from Chrysomya megacephala monomer, 10 kDa Chrysomya megacephala protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 7

Crystal and solution structures of a novel antimicrobial peptide from Chrysomya megacephala. Acta Crystallogr D Struct Biol 77(Pt 7):894-903 (2021)
Xiao C, Xiao Z, Hu C, Lu J, Cui L, Zhang Y, Dai Y, Zhang Q, Wang S, Liu W

R_g^Guinier	1.6	nm
D_max	5.0	nm
Volume^Porod	18	nm³

SASDKY6 – ParD2 antitoxin from Vibrio cholerae in low salt pH 8

Sample:	Antitoxin ParD dodecamer, 108 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Entropic pressure controls the oligomerization of the Vibrio cholerae ParD2 antitoxin Acta Crystallographica Section D Structural Biology 77(7):904-920 (2021)
Garcia-Rodriguez G, Girardin Y, Volkov A, Singh R, Muruganandam G, Van Dyck J, Sobott F, Versées W, Charlier D, Loris R

R_g^Guinier	3.4	nm
D_max	13.9	nm
Volume^Porod	190	nm³

SASDLB5 – CMT mutant i50del of human myelin protein P2

Myelin P2 disease mutant I50del experimental SAS data

Sample:	Myelin P2 disease mutant I50del monomer, 15 kDa protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jul 22

Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants. FEBS J (2021)
Uusitalo M, Klenow MB, Laulumaa S, Blakeley MP, Simonsen AC, Ruskamo S, Kursula P

R_g^Guinier	1.5	nm
D_max	4.0	nm
Volume^Porod	17	nm³

SASDLC5 – CMT mutant M114T of human myelin protein P2

Human P2 M114T mutant experimental SAS data

Sample:	Human P2 M114T mutant monomer, 15 kDa protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jul 22

R_g^Guinier	1.5	nm
D_max	4.0	nm
Volume^Porod	16	nm³

SASDLD5 – CMT mutant V115A of human myelin protein P2

Human P2 V115A mutant experimental SAS data

Sample:	Human P2 V115A mutant monomer, 15 kDa protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jul 22

R_g^Guinier	1.5	nm
D_max	4.0	nm
Volume^Porod	17	nm³

SASDGP5 – Vibrio cholerae DciA

Sample:	DciA monomer, 18 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 26

Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase. Nucleic Acids Res (2021)
Marsin S, Adam Y, Cargemel C, Andreani J, Baconnais S, Legrand P, Li de la Sierra-Gallay I, Humbert A, Aumont-Nicaise M, Velours C, Ochsenbein F, Durand D, Le Cam E, Walbott H, Possoz C, Quevillon-Cheruel S, Ferat JL

R_g^Guinier	2.7	nm
D_max	10.1	nm
Volume^Porod	26	nm³

SASDGQ5 – Vibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP)

Replicative DNA helicase (DnaB) experimental SAS data

Replicative DNA helicase (DnaB) Kratky plot

Sample:	Replicative DNA helicase (DnaB) hexamer, 317 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Oct 4

R_g^Guinier	4.6	nm
D_max	13.0	nm
Volume^Porod	585	nm³

SASDGR5 – The Vibrio cholerae DciA/DnaB helicase complex in the presence of bound ATP (VcDnaB.DciA.ATP)

DnaB helicase complexed with ATPDciA experimental SAS data

DnaB helicase complexed with ATP DciA Kratky plot

Sample:	DnaB helicase complexed with ATP hexamer, 317 kDa Vibrio cholerae serotype … protein DciA, 55 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Oct 4

R_g^Guinier	5.0	nm
D_max	15.6	nm
Volume^Porod	670	nm³

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