Small Angle Scattering Biological Data Bank SASBDB

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SASDHQ7 – Haloalkane dehalogenase variant DhaA115 - dimeric fraction

$Haloalkane dehalogenase variant DhaA115 - dimeric fraction experimental SAS data$

Sample:	Haloalkane dehalogenase variant DhaA115 - dimeric fraction dimer, 69 kDa Rhodococcus rhodochrous protein
Buffer:	50 mM potassium phosphate buffer (41 mM K₂HPO₄, 9mM KH₂PO₄), pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2019 Aug 22

Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst Chemical Science 11(41):11162-11178 (2020)
Markova K, Chmelova K, Marques S, Carpentier P, Bednar D, Damborsky J, Marek M

R_g^Guinier	2.9	nm
D_max	8.9	nm
Volume^Porod	78	nm³

SASDEN3 – Perivitellin PmPV2

P. maculata perivitellin 2 experimental SAS data

Sample:	P. maculata perivitellin 2 dimer, 188 kDa Pomacea maculata protein
Buffer:	20 mM Tris, pH: 7
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 Mar 26

Exaptation of two ancient immune proteins into a new dimeric pore-forming toxin in snails. J Struct Biol 211(2):107531 (2020)
Giglio ML, Ituarte S, Milesi V, Dreon MS, Brola TR, Caramelo J, Ip JCH, Maté S, Qiu JW, Otero LH, Heras H

R_g^Guinier	4.4	nm
D_max	14.3	nm
Volume^Porod	267	nm³

SASDES7 – Stator protein complex FlaG/FlaF

Conserved flagellar protein FStator protein FlaG soluble domain experimental SAS data

Sample:	Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV

R_g^Guinier	3.2	nm
D_max	12.5	nm
Volume^Porod	109	nm³

SASDET7 – Stator protein complex FlaG-V118K/FlaF

Conserved flagellar protein FStator protein FlaG-V118K soluble domain experimental SAS data

Sample:	Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein Stator protein FlaG-V118K soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

R_g^Guinier	3.2	nm
D_max	12.5	nm
Volume^Porod	108	nm³

SASDEU7 – Stator protein FlaG soluble domain

Conserved flagellar protein FlaG soluble domain experimental SAS data

Conserved flagellar protein FlaG soluble domain Kratky plot

Sample:	Conserved flagellar protein FlaG soluble domain monomer, 15 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

R_g^Guinier	3.7	nm
D_max	18.0	nm
Volume^Porod	133	nm³

SASDEV7 – Stator protein complex FlaG/FlaF-I96Y

Stator protein FlaG soluble domainConserved flagellar protein FlaF-I96Y soluble domain experimental SAS data

Sample:	Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein Conserved flagellar protein FlaF-I96Y soluble domain dimer, 33 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

R_g^Guinier	2.7	nm
D_max	8.2	nm
Volume^Porod	90	nm³

SASDFA9 – Poly-adenosine rA30 in 20 mM NaCl

Sample:	Poly-adenosine monomer, 10 kDa RNA
Buffer:	1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.7	nm
D_max	11.0	nm
Volume^Porod	17	nm³

SASDFB9 – Poly-adenosine rA30 in 100 mM NaCl

Sample:	Poly-adenosine monomer, 10 kDa RNA
Buffer:	1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.4	nm
D_max	10.7	nm
Volume^Porod	14	nm³

SASDFC9 – Poly-adenosine rA30 in 200 mM NaCl

Sample:	Poly-adenosine monomer, 10 kDa RNA
Buffer:	1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.5	nm
D_max	10.5	nm
Volume^Porod	14	nm³

SASDFD9 – Poly-adenosine rA30 in 400 mM NaCl

Sample:	Poly-adenosine monomer, 10 kDa RNA
Buffer:	1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	13	nm³

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