Small Angle Scattering Biological Data Bank SASBDB

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SASDF93 – Ignicoccus islandicus malate dehydrogenase

Malate dehydrogenase experimental SAS data

Sample:	Malate dehydrogenase tetramer, 134 kDa Ignicoccus islandicus DSM … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2018 Sep 5

The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization Journal of Structural Biology (2019)
Roche J, Girard E, Mas C, Madern D

R_g^Guinier	3.3	nm
D_max	9.0	nm
Volume^Porod	198	nm³

SASDF94 – Insulin glulisine (Apidra), oligomeric composition

Sample:	Insulin glulisine hexamer, 35 kDa protein
Buffer:	Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20), pH: 7.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Apr 20

The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
Nagel N, Graewert MA, Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold H

R_g^Guinier	2.3	nm
D_max	7.6	nm

SASDFA4 – Insulin glargine (Toujeo®), oligomeric composition

Insulin glargine (Toujeo®) experimental SAS data

Sample:	Insulin glargine (Toujeo®) hexamer, 36 kDa protein
Buffer:	Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 5

The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
Nagel N, Graewert MA, Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold H

R_g^Guinier	1.8	nm
D_max	6.2	nm

SASDFM5 – Mutant 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, H110A tetramer, at pH 8.5

2-amino-3-carboxymuconate 6-semialdehyde decarboxylase experimental SAS data

Sample:	2-amino-3-carboxymuconate 6-semialdehyde decarboxylase tetramer, 159 kDa Pseudomonas fluorescens protein
Buffer:	50 mM Tris, 5 mM DTT, pH: 8.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jul 15

Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity. J Biol Chem 294(30):11609-11621 (2019)
Yang Y, Davis I, Matsui T, Rubalcava I, Liu A

R_g^Guinier	5.2	nm
D_max	19.0	nm
Volume^Porod	238	nm³

SASDFN5 – Wild type 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, ACMSD tetramer, at pH 7.0

Sample:	2-amino-3-carboxymuconate 6-semialdehyde decarboxylase tetramer, 159 kDa Pseudomonas fluorescens protein
Buffer:	25 mM HEPES, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 10

R_g^Guinier	4.7	nm
D_max	17.5	nm
Volume^Porod	195	nm³

SASDF76 – Polyphosphate-targeting protein A

Polyphosphate-targeting protein A experimental SAS data

Sample:	Polyphosphate-targeting protein A dimer, 79 kDa Streptomyces chartreusis protein
Buffer:	20 mM Tris-HCl 400 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Nov 24

Structural and biochemical analysis of a phosin from Streptomyces chartreusis reveals a combined polyphosphate- and metal-binding fold. FEBS Lett (2019)
Werten S, Rustmeier NH, Gemmer M, Virolle MJ, Hinrichs W

R_g^Guinier	3.5	nm
D_max	11.8	nm
Volume^Porod	124	nm³

SASDEL6 – Glucosamine kinase from Streptacidiphilus jiangxiensis

Glucosamine kinase experimental SAS data

Sample:	Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Nov 24

Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity. MBio 10(3) (2019)
Manso JA, Nunes-Costa D, Macedo-Ribeiro S, Empadinhas N, Pereira PJB

R_g^Guinier	2.6	nm
D_max	8.0	nm
Volume^Porod	72	nm³

SASDEM6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 0.2 M D-glucosamine

Sample:	Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 0.2 M D-glucosamine, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Nov 20

Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity. MBio 10(3) (2019)
Manso JA, Nunes-Costa D, Macedo-Ribeiro S, Empadinhas N, Pereira PJB

R_g^Guinier	2.6	nm
D_max	8.0	nm
Volume^Porod	78	nm³

SASDEN6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 1 mM ATP

Sample:	Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 1 mM ATP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Nov 24

Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity. MBio 10(3) (2019)
Manso JA, Nunes-Costa D, Macedo-Ribeiro S, Empadinhas N, Pereira PJB

R_g^Guinier	2.5	nm
D_max	7.8	nm
Volume^Porod	73	nm³

SASDEP6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 0.2 M D-glucosamine and 1 mM ATP

Sample:	Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 0.2 M D-glucosamine, 1 mM ATP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Nov 20

Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity. MBio 10(3) (2019)
Manso JA, Nunes-Costa D, Macedo-Ribeiro S, Empadinhas N, Pereira PJB

R_g^Guinier	2.5	nm
D_max	7.7	nm
Volume^Porod	76	nm³

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