Browse by ORGANISM: other species

SASDDX4 – Wild-type Protein sex-lethal

Protein sex-lethal experimental SAS data
Protein sex-lethal Kratky plot
Sample: Protein sex-lethal monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 26 nm3

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

Protein sex-lethal mutant experimental SAS data
Protein sex-lethal mutant with 10GS-linker Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 2
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 27 nm3

SASDCT8 – Uncharacterized protein CTHT_0072540 (Core) from Chaetomium thermophilum

hypothetical protein CTHT_0072540 experimental SAS data
DAMMIF model
Sample: Hypothetical protein CTHT_0072540 tetramer, 62 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 30
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 4.1 nm
Dmax 16.2 nm
VolumePorod 145 nm3

SASDCU8 – Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum

hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Sample: Hypothetical protein CTHT_0072540 monomer, 35 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 19
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 2.3 nm
Dmax 5.8 nm
VolumePorod 68 nm3

SASDCV8 – Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum

Full-length hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Sample: Full-length hypothetical protein CTHT_0072540 tetramer, 207 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Oct 15
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 280 nm3

SASDC67 – SaPIbov1 pathogenicity island repressor

SaPIbov1 pathogenicity island repressor experimental SAS data
OTHER model
Sample: SaPIbov1 pathogenicity island repressor dimer, 64 kDa Staphylococcus aureus protein
Buffer: 50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 9
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor. Sci Rep 8(1):4326 (2018)
Nyíri K, Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 100 nm3

SASDC55 – Plasmodium falciparum p23A

CS domain protein, putative experimental SAS data
DAMFILT model
Sample: CS domain protein, putative monomer, 19 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26
Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. Int J Biol Macromol 108:193-204 (2018)
Silva NSM, Seraphim TV, Minari K, Barbosa LRS, Borges JC
RgGuinier 2.5 nm
Dmax 8.5 nm

SASDC65 – Plasmodium falciparum p23B

Co-chaperone p23 experimental SAS data
DAMFILT model
Sample: Co-chaperone p23 monomer, 31 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26
Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. Int J Biol Macromol 108:193-204 (2018)
Silva NSM, Seraphim TV, Minari K, Barbosa LRS, Borges JC
RgGuinier 3.7 nm
Dmax 13.0 nm

SASDCB3 – Methylated C-terminal ZBTB38 binding sequence (mCZ38BS)

methylated C-terminal ZBTB38 binding sequence experimental SAS data
DAMMIN model
Sample: Methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Oct 27
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
Pozner A, Hudson NO, Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 23 nm3

SASDLM4 – Prebiotic-producing GH10 xylanase (RmXyn10A, full length)

Prebiotic-producing GH10 xylanase (RmXyn10A, full length) experimental SAS data
BUNCH model
Sample: Prebiotic-producing GH10 xylanase (RmXyn10A, full length) monomer, 108 kDa Rhodothermus marinus protein
Buffer: 50 mM TRIS, 0.5 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Mar 12
Structural insights of RmXyn10A - A prebiotic-producing GH10 xylanase with a non-conserved aglycone binding region. Biochim Biophys Acta Proteins Proteom 1866(2):292-306 (2018)
Aronsson A, Güler F, Petoukhov MV, Crennell SJ, Svergun DI, Linares-Pastén JA, Nordberg Karlsson E
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 170 nm3