Browse by ORGANISM: other species

SASDC63 – Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide (FPV039:BAK)

Bcl-2-like protein FPV039Uncharacterized protein (BAK1) experimental SAS data
Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) Kratky plot
Sample: Bcl-2-like protein FPV039 monomer, 17 kDa Fowlpox virus protein
Uncharacterized protein (BAK1) monomer, 3 kDa Gallus gallus protein
Buffer: 20 mM trisodium citrate pH, 200 mM NaCl, pH: 6
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Oct 2
Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039. J Biol Chem 292(22):9010-9021 (2017)
Anasir MI, Caria S, Skinner MA, Kvansakul M
RgGuinier 2.0 nm

SASDBD6 – Single stranded poly-deoxythymidine DNA (30mer, dT30)

Poly-deoxythymidine (30mer) experimental SAS data
Single stranded poly-deoxythymidine DNA (30mer, dT30) Rg histogram
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
Plumridge A, Meisburger SP, Pollack L
RgGuinier 3.0 nm
Dmax 10.7 nm

SASDBE6 – Single stranded poly-deoxyadenosine DNA (30mer, dA30)

Poly-deoxyadenosine (30mer) experimental SAS data
Single stranded poly-deoxyadenosine DNA (30mer, dA30) Rg histogram
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
Plumridge A, Meisburger SP, Pollack L
RgGuinier 2.7 nm
Dmax 9.5 nm

SASDBZ8 – Single stranded poly-deoxyadenosine DNA (30mer, dA30) in 100 mM NaCl

Poly-deoxyadenosine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 100mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 16 nm3

SASDB29 – Single stranded poly-deoxyadenosine DNA (30mer, dA30) in 200 mM NaCl

Poly-deoxyadenosine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 200mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 15 nm3

SASDB39 – Single stranded poly-deoxythymidine DNA (30mer, dT30) in 100 mM NaCl

Poly-deoxythymidine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 100mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.8 nm

SASDB49 – Single stranded poly-deoxythymidine DNA (30mer, dT30) in 200 mM NaCl

Poly-deoxythymidine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 200mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.7 nm

SASDBE7 – ESX-5 type VII secretion system protein EccC5

ESX-5 type VII secretion system protein EccC5 experimental SAS data
ESX-5 type VII secretion system protein EccC5 Rg histogram
Sample: ESX-5 type VII secretion system protein EccC5 monomer, 142 kDa Mycobacterium xenopi RIVM700367 protein
Buffer: 20 mM Tris 200 mM NaCl 5%(v/v) Glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 15
Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis. Nat Microbiol 2:17047 (2017)
Beckham KS, Ciccarelli L, Bunduc CM, Mertens HD, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski MM, Svergun DI, Bitter W, Wilmanns M, Marlovits TC, Parret AH, Houben EN
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 255 nm3

SASDJB2 – Restriction endonuclease R.AgeI in apo form, monomeric

Type-2 restriction enzyme AgeI experimental SAS data
DAMMIN model
Sample: Type-2 restriction enzyme AgeI monomer, 31 kDa Thalassobius gelatinovorus protein
Buffer: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaClâ‚‚, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14
Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA. Nucleic Acids Res 45(6):3547-3558 (2017)
Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V
RgGuinier 2.2 nm
Dmax 6.7 nm
VolumePorod 53 nm3

SASDJC2 – Restriction endonuclease R.AgeI complex with cognate DNA

Type-2 restriction enzyme AgeICognate DNA oligoduplex with 5'-T overhang experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Type-2 restriction enzyme AgeI dimer, 61 kDa Thalassobius gelatinovorus protein
Cognate DNA oligoduplex with 5'-T overhang dimer, 8 kDa DNA
Buffer: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaClâ‚‚, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14
Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA. Nucleic Acids Res 45(6):3547-3558 (2017)
Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V
RgGuinier 2.4 nm
Dmax 7.4 nm
VolumePorod 69 nm3