Browse by ORGANISM: other species

SASDAM5 – Clostridium difficile bacteriophage 27 endolysin dimer C238R mutant, CD27L-C238R

Clostridium difficile bacteriophage 27 endolysin C238R mutant experimental SAS data
DAMMIF model
Sample: Clostridium difficile bacteriophage 27 endolysin C238R mutant dimer, 64 kDa Clostridioides difficile protein
Buffer: 20 mM HEPES 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 21
The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor. PLoS Pathog 10(7):e1004228 (2014)
Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 92 nm3

SASDAP4 – Chitinase 60 from Moritella marina

Chitinase 60 experimental SAS data
Chitinase 60 from Moritella marina Rg histogram
Sample: Chitinase 60 monomer, 61 kDa Moritella marina protein
Buffer: 20 mM Tris 200 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 18
Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution. Acta Crystallogr D Biol Crystallogr 70(Pt 3):676-84 (2014)
Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W
RgGuinier 3.2 nm
Dmax 11.3 nm
VolumePorod 75 nm3

SASDBF4 – Wild-type LytA (N-His6) with choline

Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 4.9 nm
Dmax 15.0 nm
VolumePorod 115 nm3

SASDBG4 – Prophage LytA with choline

Prophage Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 5.9 nm
Dmax 17.0 nm
VolumePorod 130 nm3

SASDBH4 – Wild-type LytA (N-His6) without choline

Lytic Amidase without choline experimental SAS data
SASREF model
Sample: Lytic Amidase without choline monomer, 37 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 3
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 56 nm3

SASDBJ4 – Wild-type LytA choline-binding domain

Lytic Amidase choline-binding domain experimental SAS data
SASREF model
Sample: Lytic Amidase choline-binding domain dimer, 17 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 11
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 3.3 nm
Dmax 10.0 nm
VolumePorod 49 nm3

SASDAR3 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer: 20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH: 5.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014)
Schulte T, Löfling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 37 nm3

SASDA35 – SeZinT-SeZnuA complex

High-affinity zinc transporter periplasmic componentZinc/cadmium-binding protein experimental SAS data
BUNCH model
Sample: High-affinity zinc transporter periplasmic component monomer, 33 kDa Salmonella enterica subsp. … protein
Zinc/cadmium-binding protein monomer, 23 kDa Salmonella enterica subsp. … protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2013 May 9
The Salmonella enterica ZinT structure, zinc affinity and interaction with the high-affinity uptake protein ZnuA provide insight into the management of periplasmic zinc. Biochim Biophys Acta 1840(1):535-44 (2014)
Ilari A, Alaleona F, Tria G, Petrarca P, Battistoni A, Zamparelli C, Verzili D, Falconi M, Chiancone E
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 55 nm3

SASDLT5 – Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA

Modification methylase SsoII12-bp DNA experimental SAS data
Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA Rg histogram
Sample: Modification methylase SsoII monomer, 43 kDa Shigella sonnei protein
12-bp DNA monomer, 8 kDa DNA
Buffer: 50 mM Na-phosphate buffer, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Mar 13
Flexibility of the linker between the domains of DNA methyltransferase SsoII revealed by small-angle X-ray scattering: implications for transcription regulation in SsoII restriction-modification system. PLoS One 9(4):e93453 (2014)
Konarev PV, Kachalova GS, Ryazanova AY, Kubareva EA, Karyagina AS, Bartunik HD, Svergun DI
RgGuinier 2.8 nm
Dmax 11.0 nm
VolumePorod 85 nm3

SASDB35 – Staphylococcus aureus thiaminase II

Thiaminase type II enzyme experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Thiaminase type II enzyme tetramer, 107 kDa Staphylococcus aureus protein
Buffer: 100 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 11
Staphylococcus aureus thiaminase II: oligomerization warrants proteolytic protection against serine proteases. Acta Crystallogr D Biol Crystallogr 69(Pt 12):2320-9 (2013)
Begum A, Drebes J, Kikhney A, Müller IB, Perbandt M, Svergun D, Wrenger C, Betzel C
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 168 nm3