Small Angle Scattering Biological Data Bank SASBDB


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61 hits found for Cowieson

SASDYL2 – Human prothrombin in the unbound state

Sample:	Human prothrombin monomer, 72 kDa Homo sapiens protein
Buffer:	10 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 5 mM CaCl2, 1% v/v glycerol,, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Apr 29

Prothrombin recognition and conformational modulation by anti-thrombin anticoagulant aptamers. Mol Ther (2026)
...Cowieson N, Spiridonova V, Pozzi N, Del Vecchio P, Paduano L, Sica F

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	123	nm³

SASDYM2 – Human prothrombin complex with the oligonucleotide aptamer NU172

Human prothrombinNU172 experimental SAS data

Sample:	Human prothrombin monomer, 72 kDa Homo sapiens protein NU172 monomer, 8 kDa DNA
Buffer:	10 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 5 mM CaCl2, 1% v/v glycerol,, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Apr 29

Prothrombin recognition and conformational modulation by anti-thrombin anticoagulant aptamers. Mol Ther (2026)
...Cowieson N, Spiridonova V, Pozzi N, Del Vecchio P, Paduano L, Sica F

R_g^Guinier	3.9	nm
D_max	13.8	nm
Volume^Porod	124	nm³

SASDPJ3 – IMP-1 saRNA encoding NSP1-4 of VEEV and the spike glycoprotein of SARS-CoV-2

Sample:	IMP-1 saRNA monomer, 3720 kDa RNA
Buffer:	5 mM Sodium Citrate, pH: 6.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Apr 27

Biophysical characterisation of the structure of a SARS-CoV-2 self-amplifying—RNA (saRNA) vaccine Biology Methods and Protocols (2023)
...Cowieson N, Rambo R, Shattock R

R_g^Guinier	23.8	nm
D_max	85.9	nm
Volume^Porod	6265	nm³

SASDZJ4 – Alpha-actinin-2 (ACTN2) wild-type, SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

Comprehensive biophysical and structural profiling of alpha-actinin-2 variants reveals mechanistic diversity in hypertrophic cardiomyopathy. Nat Commun 17(1) (2026)
...Cowieson N, Pinotsis N, Robinson P, Slater A, Redwood C, Loughna S, Denning C, Mohammed F, Gehmlich K

R_g^Guinier	12.1	nm
D_max	40.6	nm

SASDZK4 – Alpha-actinin-2 (ACTN2) wild-type, SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.9	nm
D_max	36.2	nm

SASDZL4 – ACTN2-R457C at 60 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.9	nm
D_max	37.0	nm

SASDZM4 – ACTN2-R457C at 53 degrees (°C) using batch-mode SAX

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.3	nm
D_max	35.3	nm

SASDZN4 – ACTN2-R457C at 40 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.3	nm
D_max	37.0	nm

SASDPP4 – Consensus SAXS Profile - Ribonuclease A

Ribonuclease pancreatic experimental SAS data

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Hammel M, Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A,...

R_g^Guinier	1.5	nm
D_max	4.9	nm
Volume^Porod	18	nm³

SASDZP4 – ACTN2-E448A at 60 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.9	nm
D_max	36.9	nm

SASDPQ4 – Consensus SAXS Profile - Urate Oxidase

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.2	nm
D_max	9.2	nm
Volume^Porod	220	nm³

SASDZQ4 – ACTN2-E448A at 53 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.6	nm
D_max	36.0	nm

SASDPR4 – Consensus SAXS Profile - Xylose Isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	ConsensusBuffer_50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.3	nm
D_max	10.1	nm
Volume^Porod	243	nm³

SASDZR4 – ACTN2-E448A at 40 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.1	nm
D_max	38.0	nm

SASDPS4 – Consensus SAXS Profile - Xylanase

Endo-1,4-beta-xylanase experimental SAS data

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.6	nm
D_max	5.1	nm
Volume^Porod	27	nm³

SASDZS4 – ACTN2-R327C at 60 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.7	nm
D_max	34.0	nm

SASDPT4 – Consensus SAXS Profile - Lysozyme

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	19	nm³

SASDZT4 – ACTN2-R327C at 53 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.4	nm
D_max	36.0	nm

SASDPU4 – Consensus SANS Profile - Ribonuclease A in 100% v/v D2O buffer

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.4	nm
D_max	4.4	nm

SASDZU4 – ACTN2-R327C at 40 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.4	nm
D_max	37.0	nm

SASDPV4 – Consensus SANS Profile - Lysozyme in 100% v/v D2O buffer

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.2	nm
D_max	3.8	nm

SASDZV4 – ACTN2-T247M at 60 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	19.1	nm
D_max	70.2	nm

SASDPW4 – Consensus SANS Profile - Xylanase in 100% v/v D2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.5	nm
D_max	4.4	nm

SASDZW4 – ACTN2-T247M at 53 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	17.0	nm
D_max	67.0	nm

SASDPX4 – Consensus SANS Profile - Urate oxidase in 100% v/v D2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.3	nm

SASDZX4 – ACTN2-T247M at 40 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	12.0	nm
D_max	37.9	nm

SASDPY4 – Consensus SANS Profile - Xylose isomerase in 100% v/v D2O buffer

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.5	nm

SASDZY4 – ACTN2-G111V at 60 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	17.9	nm
D_max	87.0	nm

SASDPZ4 – Consensus SANS Profile - Lysozyme in H2O buffer

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.4	nm
D_max	4.8	nm

SASDZZ4 – ACTN2-G111V at 53 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	13.9	nm
D_max	43.0	nm

SASDP25 – Consensus SANS Profile - Ribonuclease A in H2O buffer

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.5	nm
D_max	4.1	nm

SASDZ25 – ACTN2-G111V at 40 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	12.3	nm
D_max	38.6	nm

SASDP35 – Consensus SANS Profile - Xylanase in H2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.6	nm
D_max	4.3	nm

SASDZ35 – ACTN2-R93Q at 60 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	14.0	nm
D_max	49.3	nm

SASDP45 – Consensus SANS Profile - Urate oxidase in H2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.2	nm
D_max	9.1	nm

SASDZ45 – ACTN2-R93Q at 53 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.6	nm
D_max	36.0	nm

SASDP55 – Consensus SANS Profile - Xylose isomerase in H2O buffer

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.3	nm
D_max	9.7	nm

SASDZ55 – ACTN2-R93Q at 40 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.1	nm
D_max	37.1	nm

SASDZ65 – ACTN2-M92V at 60 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	13.5	nm
D_max	46.8	nm

SASDZ75 – ACTN2-M92V at 53 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.6	nm
D_max	35.0	nm

SASDZ85 – ACTN2-M92V at 40 degrees (°C) using batch-mode SAXS

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.6	nm
D_max	38.3	nm

SASDZ95 – Alpha-actinin-2 (ACTN2) wild-type at 60 °C

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.2	nm
D_max	37.0	nm

SASDZA5 – Alpha-actinin-2 (ACTN2) wild-type at 53 °C

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.4	nm
D_max	37.7	nm

SASDZB5 – Alpha-actinin-2 (ACTN2) wild-type at 40 °C

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.2	nm
D_max	37.6	nm

SASDZC5 – ACTN2-R457C using SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.1	nm
D_max	37.0	nm

SASDZD5 – ACTN2-E448A using SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.8	nm
D_max	36.5	nm

SASDZE5 – ACTN2-R457C using SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	12.8	nm
D_max	49.9	nm

SASDZF5 – ACTN2-E448A using SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	13.9	nm
D_max	48.1	nm

SASDZG5 – ACTN2-R327C using SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.8	nm
D_max	36.1	nm

SASDZH5 – ACTN2-R327C using SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	12.8	nm
D_max	49.1	nm

SASDZJ5 – ACTN2-T247M using SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.6	nm
D_max	37.0	nm

SASDZK5 – ACTN2-T247M using SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	16.1	nm
D_max	78.0	nm

SASDZL5 – ACTN2-G111V using SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	10.4	nm
D_max	37.0	nm

SASDZM5 – ACTN2-G111V using SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	13.0	nm
D_max	55.5	nm

SASDZN5 – ACTN2-R93Q using SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.6	nm
D_max	37.0	nm

SASDZP5 – ACTN2-R93Q using SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	12.9	nm
D_max	57.0	nm

SASDZQ5 – ACTN2-M92V using SEC-SAXS (dimer peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.5	nm
D_max	36.1	nm

SASDZR5 – Alpha-actinin-2 (ACTN2) M92V variant, SEC-SAXS (aggregate peak)

Sample:	Alpha-actinin-2 dimer, 208 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 May 13

R_g^Guinier	11.6	nm
D_max	49.0	nm

SASDTX8 – Streptococcus pyogenes glycine cleavage system H-like protein

Glycine cleavage system H-like protein experimental SAS data

Sample:	Glycine cleavage system H-like protein monomer, 13 kDa Streptococcus pyogenes serotype … protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 5% (v/v) glycerol, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2016 Jul 20

Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains Journal of Biological Chemistry :107770 (2024)
...Cowieson N, Ahel I, Filippov D, Rack J

R_g^Guinier	1.5	nm
D_max	5.0	nm
Volume^Porod	22	nm³

SASDTY8 – Streptococcus pyogenes Protein-ADP-ribose hydrolase

Protein-ADP-ribose hydrolase (D13G, Y23S, T61A, I114S, R177H, I246T) experimental SAS data

Sample:	Protein-ADP-ribose hydrolase (D13G, Y23S, T61A, I114S, R177H, I246T) monomer, 30 kDa Streptococcus pyogenes serotype … protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 5% (v/v) glycerol, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2016 Jul 20

Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains Journal of Biological Chemistry :107770 (2024)
...Cowieson N, Ahel I, Filippov D, Rack J

R_g^Guinier	2.1	nm
D_max	7.3	nm
Volume^Porod	34	nm³

SASDTZ8 – Streptococcus pyogenes Protein-ADP-ribose hydrolase: glycine cleavage system H-like protein complex

Glycine cleavage system H-like proteinProtein-ADP-ribose hydrolase (D13G, Y23S, T61A, I114S, R177H, I246T) experimental SAS data

Sample:	Glycine cleavage system H-like protein monomer, 13 kDa Streptococcus pyogenes serotype … protein Protein-ADP-ribose hydrolase (D13G, Y23S, T61A, I114S, R177H, I246T) monomer, 30 kDa Streptococcus pyogenes serotype … protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 5% (v/v) glycerol, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2016 Jul 20

Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains Journal of Biological Chemistry :107770 (2024)
...Cowieson N, Ahel I, Filippov D, Rack J

R_g^Guinier	2.4	nm
D_max	8.4	nm
Volume^Porod	53	nm³

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