Small Angle Scattering Biological Data Bank SASBDB


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53 hits found for Sagar

SASDKA2 – Two titin I27 domains linked with RS linker

Two titin I27 domains linked with RS linker experimental SAS data

Two titin I27 domains linked with RS linker Rg histogram

Sample:	Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer:	50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
...Sagar A, Rakshit S

R_g^Guinier

3.0

SASDKB2 – Two titin I27 domains linked with RS linker

Sample:	Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer:	50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
...Sagar A, Rakshit S

R_g^Guinier

3.0

SASDKC2 – Two titin I27 domains linked with GGG linker

Two titin I27 domains linked with GGG linker experimental SAS data

Two titin I27 domains linked with GGG linker Rg histogram

Sample:	Two titin I27 domains linked with GGG linker monomer, 23 kDa protein
Buffer:	50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
...Sagar A, Rakshit S

R_g^Guinier

2.9

SASDKD2 – Two titin I27 domains linked with VKA linker

Two titin I27 domains linked with VKA linker experimental SAS data

Two titin I27 domains linked with VKA linker Rg histogram

Sample:	Two titin I27 domains linked with VKA linker monomer, 24 kDa protein
Buffer:	50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
...Sagar A, Rakshit S

R_g^Guinier

3.1

SASDKE2 – Two titin I27 domains linked with AAE linker

Two titin I27 domains linked with AAE linker experimental SAS data

Two titin I27 domains linked with AAE linker Rg histogram

Sample:	Two titin I27 domains linked with AAE linker monomer, 24 kDa protein
Buffer:	50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
...Sagar A, Rakshit S

R_g^Guinier

3.1

SASDXM2 – RAR/RXR heterodimer : BMS493 : DR0 element

Retinoic acid receptor alphaDirect Repeat binding site with zero-base-pair spacingBMS493 (RAR inverse agonist ligand)Retinoid X receptor alpha experimental SAS data

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein Direct Repeat binding site with zero-base-pair spacing monomer, 10 kDa DNA BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2024 Apr 18

New structural insights into the control of the retinoic acid receptors RAR/RXR by DNA, ligands, and transcriptional coregulators. Nucleic Acids Res 53(18) (2025)
...Sagar A, Vankova P, Loginov D, Carivenc C, Rochel N, Bourguet W, Man P, Bernadó P, le Maire A

R_g^Guinier	3.6	nm
D_max	13.1	nm
Volume^Porod	121	nm³

SASDXN2 – RAR/RXR heterodimer : BMS493 : DR0L element

Retinoic acid receptor alphaBMS493 (RAR inverse agonist ligand)Direct Repeat binding site with zero-base-pair spacing (long sequence)Retinoid X receptor alpha experimental SAS data

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Direct Repeat binding site with zero-base-pair spacing (long sequence) monomer, 19 kDa DNA Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 12

R_g^Guinier	4.1	nm
D_max	14.8	nm
Volume^Porod	152	nm³

SASDXP2 – RAR/RXR heterodimer : BMS493 : DR1 element

Retinoic acid receptor alphaBMS493 (RAR inverse agonist ligand)Retinoid X receptor alphaDirect Repeat binding site with one-base-pair spacing experimental SAS data

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with one-base-pair spacing monomer, 11 kDa DNA
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 20

R_g^Guinier	3.8	nm
D_max	13.9	nm
Volume^Porod	120	nm³

SASDXQ2 – RAR/RXR heterodimer : BMS493 : DR5 element

Retinoic acid receptor alphaBMS493 (RAR inverse agonist ligand)Retinoid X receptor alphaDirect Repeat binding site with five-base-pair spacing experimental SAS data

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with five-base-pair spacing monomer, 13 kDa DNA
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 12

R_g^Guinier	3.9	nm
D_max	14.3	nm
Volume^Porod	130	nm³

SASDXR2 – RAR/RXR heterodimer : BMS493 : IR0 element

Retinoic acid receptor alphaBMS493 (RAR inverse agonist ligand)Retinoid X receptor alphaInverted Repeat binding site with zero-base-pair spacing experimental SAS data

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Inverted Repeat binding site with zero-base-pair spacing monomer, 11 kDa DNA
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 20

R_g^Guinier	3.9	nm
D_max	12.9	nm
Volume^Porod	121	nm³

SASDXS2 – RAR/RXR heterodimer : BMS493 : NCoR NID : DR0L element

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Direct Repeat binding site with zero-base-pair spacing (long sequence) monomer, 19 kDa DNA Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 12

R_g^Guinier	5.4	nm
D_max	20.1	nm
Volume^Porod	363	nm³

SASDXT2 – RAR/RXR heterodimer : BMS493 : NCoR NID : DR1 element

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with one-base-pair spacing monomer, 11 kDa DNA Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 20

R_g^Guinier	5.6	nm
D_max	21.8	nm
Volume^Porod	330	nm³

SASDXU2 – RAR/RXR heterodimer : BMS493 : NCoR NID : DR5 element

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with five-base-pair spacing monomer, 13 kDa DNA Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 12

R_g^Guinier	5.7	nm
D_max	21.8	nm
Volume^Porod	363	nm³

SASDXV2 – RAR/RXR heterodimer : BMS493 : NCoR NID : IR0 element

Sample:	Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Inverted Repeat binding site with zero-base-pair spacing monomer, 11 kDa DNA Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein
Buffer:	20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 20

R_g^Guinier	5.5	nm
D_max	22.9	nm
Volume^Porod	257	nm³

SASDUW3 – Calcium activated full-length gelsolin

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, pH8, 45 mM NaCl, 1 mM EGTA + 4 mM CaCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish

R_g^Guinier	4.2	nm
D_max	16.0	nm
Volume^Porod	129	nm³

SASDUX3 – F-actin in F-buffer at an actin concentration of 2 mg/mL

Actin, cytoplasmic 1 experimental SAS data

Sample:	Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer:	50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier	13.4	nm
D_max	48.0	nm

SASDUY3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:32 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data

Gelsolin Actin, cytoplasmic 1 Kratky plot

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer:	50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier

12.2

SASDUZ3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:16 molar ratio in F-actin buffer

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer:	50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier

14.5

SASDU24 – Calcium activated full-length gelsolin and the F-form of actin at a 1:8 molar ratio in F-actin buffer

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer:	50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier

13.6

SASDU34 – Calcium activated full-length gelsolin and the F-form of actin at a 1:4 molar ratio in F-actin buffer

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer:	50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier

8.8

SASDU44 – Calcium activated full-length gelsolin and the F-form of actin at a 1:2 molar ratio in F-actin buffer

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer:	50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier

7.3

SASDU54 – Calcium activated full-length gelsolin and the F-form of actin at a 1:1 molar ratio in F-actin buffer

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer:	50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier

5.2

SASDU64 – Calcium-gelsolin and the F-form of actin at a 1:2 molar ratio from high to low ionic strength

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer:	2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier	4.7	nm
D_max	25.0	nm
Volume^Porod	262	nm³

SASDU74 – Calcium-gelsolin and the F-form of actin at a 1:1 molar ratio from high to low ionic strength

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer:	2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier	5.2	nm
D_max	25.0	nm
Volume^Porod	266	nm³

SASDU84 – Calcium-gelsolin and the G-form of actin at a 1:2 molar ratio in low ionic strength (SEC-SAXS)

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer:	2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31

R_g^Guinier	4.4	nm
D_max	25.0	nm
Volume^Porod	241	nm³

SASDU94 – Calcium-gelsolin and the G-form of actin at a 1:1 molar ratio in low ionic strength (SEC-SAXS)

Sample:	Gelsolin monomer, 84 kDa Homo sapiens protein Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer:	2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Sep 1

R_g^Guinier	4.6	nm
D_max	25.0	nm
Volume^Porod	243	nm³

SASDNK6 – Retinoblastoma protein at 1 mg/ml

Retinoblastoma-associated protein experimental SAS data

Retinoblastoma-associated protein Kratky plot

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

Conformational buffering underlies functional selection in intrinsically disordered protein regions. Nat Struct Mol Biol (2022)
...Sagar A, Estaña A, Barozet A, Garrone NA, Fernandez-Ballester G, Blanes-Mira C, Sánchez IE, de Prat-Gay G, Cortés J, Bernadó P, Pappu RV, Holehouse AS, Daughdrill GW, Chemes LB

R_g^Guinier	2.4	nm
D_max	7.4	nm
Volume^Porod	66	nm³

SASDNL6 – Retinoblastoma protein at 2 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	2.5	nm
D_max	7.9	nm
Volume^Porod	64	nm³

SASDNM6 – Retinoblastoma protein at 4 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	2.6	nm
D_max	10.0	nm
Volume^Porod	68	nm³

SASDNN6 – Early E1A protein at 4.2 mg/ml

Sample:	Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 24

R_g^Guinier

4.0

SASDNP6 – Early E1A protein at 5.6 mg/ml

Sample:	Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 24

R_g^Guinier

4.2

SASDNQ6 – Early E1A protein at 7.0 mg/ml

Sample:	Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 24

R_g^Guinier

4.1

SASDNR6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 0.7 mg/ml

Retinoblastoma-associated proteinEarly E1A protein experimental SAS data

Retinoblastoma-associated protein Early E1A protein Kratky plot

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	80	nm³

SASDNS6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 1.4 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDNT6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 2.7 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.3	nm
D_max	17.0	nm
Volume^Porod	93	nm³

SASDNU6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex - merged data from concentration series measurements

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDNV6 – Early E1A protein, SEC-SAXS data

Sample:	Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 24

R_g^Guinier	3.6	nm
D_max	17.5	nm
Volume^Porod	45	nm³

SASDGY6 – Salt stress-induced protein at a sample concentration of 5mg/ml

Salt stress-induced protein experimental SAS data

Sample:	Salt stress-induced protein dimer, 37 kDa Oryza sativa Indica … protein
Buffer:	50 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2017 Mar 6

Structural insights into rice SalTol QTL located SALT protein. Sci Rep 10(1):16589 (2020)
...Sagar A, Sharma P, Ashish, Pati PK

R_g^Guinier	2.5	nm
D_max	6.7	nm
Volume^Porod	56	nm³

SASDGZ6 – Salt stress-induced protein at a sample concentration of 11mg/ml

Sample:	Salt stress-induced protein dimer, 37 kDa Oryza sativa Indica … protein
Buffer:	50 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2017 Mar 6

Structural insights into rice SalTol QTL located SALT protein. Sci Rep 10(1):16589 (2020)
...Sagar A, Sharma P, Ashish, Pati PK

R_g^Guinier	2.4	nm
D_max	6.6	nm
Volume^Porod	60	nm³

SASDG27 – Salt stress-induced protein at a sample concentration of 22mg/ml

Sample:	Salt stress-induced protein dimer, 37 kDa Oryza sativa Indica … protein
Buffer:	50 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2017 Mar 6

Structural insights into rice SalTol QTL located SALT protein. Sci Rep 10(1):16589 (2020)
...Sagar A, Sharma P, Ashish, Pati PK

R_g^Guinier	2.5	nm
D_max	6.5	nm
Volume^Porod	57	nm³

SASDHF8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data

Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K Rg histogram

Sample:	Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13

Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
Sagar A, Jeffries C, Petoukhov M, Svergun D, Bernadó P

R_g^Guinier	1.4	nm
D_max	6.0	nm
Volume^Porod	3	nm³

SASDHG8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 2.51 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide Kratky plot

Sample:	Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer:	20 mM Tris 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13

R_g^Guinier	1.5	nm
D_max	6.4	nm
Volume^Porod	3	nm³

SASDHH8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 1.26 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Sample:	Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer:	20 mM Tris 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13

R_g^Guinier	1.5	nm
D_max	6.0	nm
Volume^Porod	3	nm³

SASDQR8 – Exon1 of Non pathogenic form of Huntingtin (H16) with GFP fused at the C-terminus

Sample:	Huntingtin monomer, 39 kDa Homo sapiens protein
Buffer:	20mM BisTris-HCl, 150mM NaCl, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 3

The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity. Nat Struct Mol Biol (2023)
...Sagar A, Urbanek A, Popovic M, Morató A, Estaña A, Fournet A, Doucet C, Lund XL, Shi ZD, Costa L, Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortés J, Sinnaeve D, Sibille N,...

R_g^Guinier	3.3	nm
D_max	16.0	nm
Volume^Porod	63	nm³

SASDQS8 – Exon1 of Pathogenic form of Huntingtin (H46) with GFP fused at the C-terminus

Sample:	Huntingtin monomer, 43 kDa Homo sapiens protein
Buffer:	20mM BisTris-HCl, 150mM NaCl, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Oct 28

R_g^Guinier

4.2

SASDKT8 – von Willebrand Factor peptide 3mg/ml

von Willebrand factor experimental SAS data

Sample:	von Willebrand factor monomer, 11 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24

An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution. J Mol Biol 433(13):166954 (2021)
...Sagar A, Pompach P, Goulas T, Scavenius C, Ferrero DS, Castrillo-Briceño M, Taulés M, Enghild JJ, Bernadó P, Gomis-Rüth FX

R_g^Guinier	3.1	nm
D_max	14.0	nm
Volume^Porod	28	nm³

SASDKU8 – von Willebrand Factor peptide 6mg/ml

von Willebrand Factor peptide 6mg/ml Rg histogram

Sample:	von Willebrand factor monomer, 11 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24

R_g^Guinier	3.0	nm
D_max	14.9	nm
Volume^Porod	27	nm³

SASDKV8 – ADAMTS-13 bound to von Willebrand Factor peptide

von Willebrand factorA disintegrin and metalloproteinase with thrombospondin motifs 13 experimental SAS data

ADAMTS-13 bound to von Willebrand Factor peptide Rg histogram

Sample:	von Willebrand factor monomer, 11 kDa Homo sapiens protein A disintegrin and metalloproteinase with thrombospondin motifs 13 monomer, 70 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24

R_g^Guinier	4.4	nm
D_max	16.3	nm
Volume^Porod	170	nm³

SASDLW2 – Accessory colonization factor SslE at pH 4.4

Accessory colonization factor experimental SAS data

Sample:	Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein
Buffer:	20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 4.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 27

Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
...Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J

R_g^Guinier	3.9	nm
D_max	13.7	nm
Volume^Porod	248	nm³

SASDLV2 – Accessory colonization factor SslE at pH 7.4

Sample:	Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein
Buffer:	20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 27

R_g^Guinier	4.0	nm
D_max	14.1	nm
Volume^Porod	244	nm³

SASDMU6 – The N1 domain of accessory colonization factor SslE

Sample:	Accessory colonization factor monomer, 17 kDa Escherichia coli (strain … protein
Buffer:	20 mM Tris, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Apr 21

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDMV6 – The N2 domain of accessory colonization factor SslE

Sample:	Accessory colonization factor monomer, 23 kDa Escherichia coli (strain … protein
Buffer:	20 mM Tris, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Apr 21

R_g^Guinier	2.2	nm
D_max	7.7	nm
Volume^Porod	42	nm³

SASDMW6 – The N3-M60 domain of accessory colonization factor SslE

Sample:	Accessory colonization factor monomer, 121 kDa Escherichia coli (strain … protein
Buffer:	20 mM Tris, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Apr 21

R_g^Guinier	3.4	nm
D_max	10.5	nm
Volume^Porod	171	nm³

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