SASBDB entries for UniProt ID:

SASDMJ9 – Plasmid pET11d- 229E710 encoding C-terminally His6-tagged HCoV-229E Nsp7-10 polyprotein

UniProt ID: P0C6U8 (3837-3919) Replicase polyprotein 1a
Replicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
RgGuinier 2.1 nm

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

UniProt ID: P0C6U8 (3837-3919) Replicase polyprotein 1a

UniProt ID: P0C6U8 (3943-4140) Replicase polyprotein 1a
Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
RgGuinier 3.0 nm

SASDML9 – Structure of bifunctional protease-helicase NS3/4A domain assembly in solution

UniProt ID: P26663 (None-None) Genome polyprotein
Genome polyprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Genome polyprotein dimer, 137 kDa Hepatitis C virus … protein
Buffer: 25 mM Tris, 1 M NaCl, 10% glycerol, 1 mM TCEP, 0.1% β-octyl glucoside, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 12
A macrocyclic HCV NS3/4A protease inhibitor interacts with protease and helicase residues in the complex with its full-length target Proceedings of the National Academy of Sciences 108(52):21052-21056 (2011)
Schiering N, D'Arcy A, Villard F, Simic O, Kamke M, Monnet G, Hassiepen U, Svergun D, Pulfer R, Eder J, Raman P, Bodendorf U
RgGuinier 3.9 nm

SASDMM9 – Response Regulator RocR from Pseudomonas aeruginosa

UniProt ID: Q9HX69 (None-None) RocR
RocR experimental SAS data
OTHER model
Sample: RocR tetramer, 171 kDa Pseudomonas aeruginosa (strain … protein
Buffer: 50 mM Tris–HCl, 250 mM NaCl, 10 mM imidazole, 5% glycerol, 0.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 9
Structural Insights into the Regulatory Mechanism of the Response Regulator RocR from Pseudomonas aeruginosa in Cyclic Di-GMP Signaling Journal of Bacteriology 194(18):4837-4846 (2012)
Chen M, Kotaka M, Vonrhein C, Bricogne G, Rao F, Chuah M, Svergun D, Schneider G, Liang Z, Lescar J
RgGuinier 3.7 nm
Dmax 11.0 nm

SASDMN9 – Conformational characterization of A77-78 fibronectin type III tandem

UniProt ID: Q8WZ42 (None-None) Titin
Titin experimental SAS data
OTHER model
Sample: Titin monomer, 22 kDa Homo sapiens protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 3
The Structure of the FnIII Tandem A77-A78 Points to a Periodically Conserved Architecture in the Myosin-Binding Region of Titin Journal of Molecular Biology 401(5):843-853 (2010)
Bucher R, Svergun D, Muhle-Goll C, Mayans O
RgGuinier 2.5 nm
Dmax 90.0 nm
VolumePorod 21 nm3

SASDMP9 – Conformational characterization of A80-82 fibronectin type III tandem

UniProt ID: Q8WZ42 (None-None) Titin
Titin experimental SAS data
SASREF model
Sample: Titin monomer, 32 kDa Homo sapiens protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 3
The Structure of the FnIII Tandem A77-A78 Points to a Periodically Conserved Architecture in the Myosin-Binding Region of Titin Journal of Molecular Biology 401(5):843-853 (2010)
Bucher R, Svergun D, Muhle-Goll C, Mayans O
RgGuinier 3.7 nm
Dmax 130.0 nm
VolumePorod 39 nm3

SASDMQ9 – Conformational characterization of A84-86 fibronectin type III tandem

UniProt ID: Q8WZ42 (None-None) Titin
Titin experimental SAS data
SASREF model
Sample: Titin monomer, 32 kDa Homo sapiens protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Oct 6
The Structure of the FnIII Tandem A77-A78 Points to a Periodically Conserved Architecture in the Myosin-Binding Region of Titin Journal of Molecular Biology 401(5):843-853 (2010)
Bucher R, Svergun D, Muhle-Goll C, Mayans O
RgGuinier 3.7 nm
Dmax 14.0 nm

SASDMX9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1

UniProt ID: A0A2N0URA4 (32-269) Dockerin domain-containing protein
Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 2.1 nm
Dmax 10.3 nm
VolumePorod 42 nm3

SASDMY9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2

UniProt ID: A0A2N0URA4 (311-556) Dockerin domain-containing protein
Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 35 nm3

SASDMZ9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2

UniProt ID: A0A2N0URA4 (27-559) Dockerin domain-containing protein
Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 57 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 5.4 nm
Dmax 20.3 nm
VolumePorod 93 nm3