SASBDB entries for UniProt ID:

SASDRZ9 – Regulator of telomere elongation helicase 1, HHD-1 domain

UniProt ID: Q9NZ71-6 (889-974) Regulator of telomere elongation helicase 1 (Isoform 6, 889-974)
Regulator of telomere elongation helicase 1 (Isoform 6, 889-974) experimental SAS data
ALPHAFOLD model
Sample: Regulator of telomere elongation helicase 1 (Isoform 6, 889-974) monomer, 10 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl,10 (v/v)% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 22
Structural and biochemical characterization of the C‐terminal region of the human RTEL 1 helicase Protein Science 33(9) (2024)
Cortone G, Graewert M, Kanade M, Longo A, Hegde R, González‐Magaña A, Chaves‐Arquero B, Blanco F, Napolitano L, Onesti S
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 22 nm3

SASDSP2 – Saccharomyces cerevisiae Mer2 coiled-coiled with N-terminal SUMO-tag

UniProt ID: P21651 (44-224) Recombination protein 107
Recombination protein 107 experimental SAS data
Sample: Recombination protein 107 tetramer, 139 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 8.3 nm
Dmax 32.2 nm

SASDSZ5 – DNA binding domain of PALB2 (partner and localizer of BRCA2) in 500 mM NaCl

UniProt ID: Q86YC2 (1-200) Partner and localizer of BRCA2
Partner and localizer of BRCA2 experimental SAS data
DNA binding domain of PALB2 (partner and localizer of BRCA2) in 500 mM NaCl Rg histogram
Sample: Partner and localizer of BRCA2 dimer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 11
The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction. iScience 27(12):111259 (2024)
Kyriukha Y, Watkins MB, Redington JM, Chintalapati N, Ganti A, Dastvan R, Uversky VN, Hopkins JB, Pozzi N, Korolev S
RgGuinier 5.5 nm
Dmax 25.4 nm
VolumePorod 171 nm3

SASDSJ7 – Human C-terminally truncated 14-3-3 gamma

UniProt ID: P61981 (1-234) 14-3-3 protein gamma
14-3-3 protein gamma experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 76 nm3

SASDSK7 – Tandem of WW domains of Pre-mRNA-processing factor 40 homolog A (PRPF40A)

UniProt ID: O75400 (141-236) Pre-mRNA-processing factor 40 homolog A
Pre-mRNA-processing factor 40 homolog A experimental SAS data
Pre-mRNA-processing factor 40 homolog A Kratky plot
Sample: Pre-mRNA-processing factor 40 homolog A monomer, 11 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 100 mM NaCl, 0.5 mM DTT, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Sep 19
Intramolecular autoinhibition regulates the selectivity of PRPF40A tandem WW domains for proline-rich motifs. Nat Commun 15(1):3888 (2024)
Martínez-Lumbreras S, Träger LK, Mulorz MM, Payr M, Dikaya V, Hipp C, König J, Sattler M
RgGuinier 2.0 nm
Dmax 6.4 nm
VolumePorod 17 nm3

SASDSZ9 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 238 µM

UniProt ID: P0DTC9 (176-245) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 19 nm3

SASDTY2 – Teneurin-3 A1B1 isoform - 0.97 mg/mL

UniProt ID: Q9WTS6-1 (343-2715) Isoform A1B1 of Teneurin-3
Isoform A1B1 of Teneurin-3 experimental SAS data
Isoform A1B1 of Teneurin-3 Kratky plot
Sample: Isoform A1B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10
Alternative splicing controls teneurin-3 compact dimer formation for neuronal recognition Nature Communications 15(1) (2024)
Gogou C, Beugelink J, Frias C, Kresik L, Jaroszynska N, Drescher U, Janssen B, Hindges R, Meijer D
RgGuinier 8.9 nm
Dmax 37.0 nm
VolumePorod 1059 nm3

SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

UniProt ID: E7EDN2 (27-483) Variable surface glycoprotein LiTat 1.5
Variable surface glycoprotein LiTat 1.5 experimental SAS data
BILBOMD model
Sample: Variable surface glycoprotein LiTat 1.5 dimer, 98 kDa Trypanosoma brucei gambiense protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 170 nm3

SASDUU5 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 bound to 3'UTR fragment of ADP-ribosylation factor 1

UniProt ID: O95793 (178-577) Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-177)

UniProt ID: None (None-None) 3'UTR fragment of ADP-ribosylation factor 1
Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-177)3'UTR fragment of ADP-ribosylation factor 1 experimental SAS data
CORAL model
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-177) dimer, 89 kDa Homo sapiens protein
3'UTR fragment of ADP-ribosylation factor 1 monomer, 14 kDa Homo sapiens RNA
Buffer: 50 mM TRIS, 300 mM NaCl, 3.8 mM β-mercaptoethanol, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2020 May 4
A Simple Protocol for Visualization of RNA-Protein Complexes by Atomic Force Microscopy. Curr Protoc 5(1):e70084 (2025)
Tripepi A, Shakoor H, Klapetek P
RgGuinier 4.9 nm
Dmax 13.4 nm
VolumePorod 129 nm3

SASDUL9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

UniProt ID: J3QLL0 (2-73) Importin subunit alpha-1
Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 11.6 nm
VolumePorod 23 nm3