SASBDB entries for UniProt ID:

SASDWP5 – N-acetylmuramoyl-L-alanine amidase (with His-Tag)

UniProt ID: G8I4E0 (1-395) N-acetylmuramoyl-L-alanine amidase (Mycobacteriophage DS6A)
N-acetylmuramoyl-L-alanine amidase (Mycobacteriophage DS6A) experimental SAS data
COOT model
Sample: N-acetylmuramoyl-L-alanine amidase (Mycobacteriophage DS6A) monomer, 45 kDa protein
Buffer: 20 mM Tris, 150 mM NaCl and 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 30
Dissecting the molecular basis underlying mycobacterial cell-wall hydrolysis by the catalytic domains of D29LysA and DS6ALysA phage endolysins. Int J Biol Macromol :148896 (2025)
Ceballos-Zúñiga F, Galvez-Larrosa L, Muñoz IG, Infantes L, Fernández-Carrillo J, Pérez-Dorado I
RgGuinier 2.8 nm
Dmax 10.3 nm
VolumePorod 62 nm3

SASDWQ5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV)

UniProt ID: Q88595 (923-1055) Genome polyprotein
Genome polyprotein experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Buffer: 20 mM HEPES pH 7.4, 1 M KCl, 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
A new protein-dependent riboswitch activates ribosomal frameshifting (2025)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 30 nm3

SASDWS5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

UniProt ID: Q88595 (923-1055) Genome polyprotein

UniProt ID: None (None-None) Stimulatory element RNA
Genome polyproteinStimulatory element RNA experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
A new protein-dependent riboswitch activates ribosomal frameshifting (2025)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 46 nm3

SASDWT5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) SEC-SAXS

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein (BRC repeat 4)

UniProt ID: Q06609 (98-339) DNA repair protein RAD51 homolog 1 (Δ97)
Breast cancer type 2 susceptibility protein (BRC repeat 4)DNA repair protein RAD51 homolog 1 (Δ97) experimental SAS data
ALPHAFOLD model
Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 57 nm3

SASDWU5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 1 mg/mL

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein (BRC repeat 4)

UniProt ID: Q06609 (98-339) DNA repair protein RAD51 homolog 1 (Δ97)
Breast cancer type 2 susceptibility protein (BRC repeat 4)DNA repair protein RAD51 homolog 1 (Δ97) experimental SAS data
Breast cancer type 2 susceptibility protein (BRC repeat 4) DNA repair protein RAD51 homolog 1 (Δ97) Kratky plot
Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 51 nm3

SASDWV5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 2 mg/mL

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein (BRC repeat 4)

UniProt ID: Q06609 (98-339) DNA repair protein RAD51 homolog 1 (Δ97)
Breast cancer type 2 susceptibility protein (BRC repeat 4)DNA repair protein RAD51 homolog 1 (Δ97) experimental SAS data
Breast cancer type 2 susceptibility protein (BRC repeat 4) DNA repair protein RAD51 homolog 1 (Δ97) Kratky plot
Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 49 nm3

SASDWW5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 4 mg/mL

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein (BRC repeat 4)

UniProt ID: Q06609 (98-339) DNA repair protein RAD51 homolog 1 (Δ97)
Breast cancer type 2 susceptibility protein (BRC repeat 4)DNA repair protein RAD51 homolog 1 (Δ97) experimental SAS data
Breast cancer type 2 susceptibility protein (BRC repeat 4) DNA repair protein RAD51 homolog 1 (Δ97) Kratky plot
Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.1 nm
Dmax 6.3 nm
VolumePorod 57 nm3

SASDWX5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 6 mg/mL

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein (BRC repeat 4)

UniProt ID: Q06609 (98-339) DNA repair protein RAD51 homolog 1 (Δ97)
Breast cancer type 2 susceptibility protein (BRC repeat 4)DNA repair protein RAD51 homolog 1 (Δ97) experimental SAS data
Breast cancer type 2 susceptibility protein (BRC repeat 4) DNA repair protein RAD51 homolog 1 (Δ97) Kratky plot
Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 63 nm3

SASDWY5 – RAD51 [F86E, A89E] in complex with fourth BRC repeat (BRC4) SEC-SAXS

UniProt ID: Q06609 (1-339) DNA repair protein RAD51 homolog 1 (F86E A89E)

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein (BRC repeat 4)
DNA repair protein RAD51 homolog 1 (F86E A89E)Breast cancer type 2 susceptibility protein (BRC repeat 4) experimental SAS data
ALPHAFOLD model
Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein
Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 74 nm3

SASDWZ5 – RAD51 [F86E, A89E] in complex with fourth BRC repeat (BRC4) at 0.5 mg/mL

UniProt ID: Q06609 (1-339) DNA repair protein RAD51 homolog 1 (F86E A89E)

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein (BRC repeat 4)
DNA repair protein RAD51 homolog 1 (F86E A89E)Breast cancer type 2 susceptibility protein (BRC repeat 4) experimental SAS data
DNA repair protein RAD51 homolog 1 (F86E A89E) Breast cancer type 2 susceptibility protein (BRC repeat 4) Kratky plot
Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein
Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.8 nm
Dmax 11.0 nm
VolumePorod 67 nm3