SASBDB entries for UniProt ID:

SASDWJ7 – Wild type ferric binding protein (FbpA) in apo (no Fe) form in high ionic strength (HIS) buffer (PBS)

UniProt ID: P35755 (23-332) Iron-utilization periplasmic protein
Iron-utilization periplasmic protein experimental SAS data
DAMFILT model
Sample: Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer: 10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 9
Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations The Journal of Chemical Physics 158(8) (2023)
Liu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 39 nm3

SASDWK7 – D52A mutant of ferric binding protein (FbpA) in holo (with Fe) form in high ionic strength (HIS) buffer (PBS)

UniProt ID: P35755 (23-332) Iron-utilization periplasmic protein
Iron-utilization periplasmic protein experimental SAS data
DAMMIN model
Sample: Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer: 10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 9
Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations The Journal of Chemical Physics 158(8) (2023)
Liu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z
RgGuinier 2.0 nm
Dmax 5.9 nm
VolumePorod 33 nm3

SASDWL7 – D52A mutant of ferric binding protein (FbpA) in apo (no Fe) form in high ionic strength (HIS) buffer (PBS)

UniProt ID: P35755 (23-332) Iron-utilization periplasmic protein
Iron-utilization periplasmic protein experimental SAS data
DAMMIN model
Sample: Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer: 10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 19
Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations The Journal of Chemical Physics 158(8) (2023)
Liu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z
RgGuinier 2.1 nm
Dmax 6.2 nm
VolumePorod 40 nm3

SASDWM7 – D52A mutant of ferric binding protein (FbpA) in holo (with Fe) form in low ionic strength (LIS) buffer (1/10 PBS)

UniProt ID: P35755 (23-332) Iron-utilization periplasmic protein
Iron-utilization periplasmic protein experimental SAS data
DAMFILT model
Sample: Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer: 1 mM Na2HPO4.7H2O, 0.18 mM KH2PO4, 13.7 mM NaCl, 0.27 mM KCl, 5%v/v Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20
Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations The Journal of Chemical Physics 158(8) (2023)
Liu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z
RgGuinier 2.0 nm
Dmax 6.1 nm
VolumePorod 24 nm3

SASDWN7 – Wild type ferric binding protein (FbpA) in apo (no Fe) form in low ionic strength (LIS) buffer (1/10 PBS)

UniProt ID: P35755 (23-332) Iron-utilization periplasmic protein
Iron-utilization periplasmic protein experimental SAS data
DAMFILT model
Sample: Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer: 1 mM Na2HPO4.7H2O, 0.18 mM KH2PO4, 13.7 mM NaCl, 0.27 mM KCl, 5%v/v Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20
Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations The Journal of Chemical Physics 158(8) (2023)
Liu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z
RgGuinier 2.1 nm
Dmax 6.3 nm
VolumePorod 35 nm3

SASDWP7 – Wild type ferric binding protein (FbpA) in holo (with Fe) form in low ionic strength (LIS) buffer (1/10 PBS)

UniProt ID: P35755 (23-332) Iron-utilization periplasmic protein
Iron-utilization periplasmic protein experimental SAS data
DAMMIN model
Sample: Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer: 1 mM Na2HPO4.7H2O, 0.18 mM KH2PO4, 13.7 mM NaCl, 0.27 mM KCl, 5%v/v Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20
Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations The Journal of Chemical Physics 158(8) (2023)
Liu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z
RgGuinier 2.0 nm
Dmax 6.1 nm
VolumePorod 29 nm3

SASDWQ7 – Teneurin-3 wildtype A0B0 isoform in 2 mM calcium buffer - 1.0 mg/ml

UniProt ID: Q9WTS6-2 (343-2699) Isoform A0B0 of Teneurin-3 wildtype
Isoform A0B0 of Teneurin-3 wildtype experimental SAS data
Isoform A0B0 of Teneurin-3 wildtype Kratky plot
Sample: Isoform A0B0 of Teneurin-3 wildtype dimer, 533 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2024 Dec 5
Teneurin-3 isoforms, WT and R2579W
Maja Napieraj
RgGuinier 8.0 nm
Dmax 39.3 nm
VolumePorod 1289 nm3

SASDWR7 – Teneurin-3 mutant A0B0 isoform in 2 mM calcium buffer - 1.04 mg/ml

UniProt ID: Q9WTS6-2 (343-2699) Isoform A0B0 of Teneurin-3 R2579W mutant
Isoform A0B0 of Teneurin-3 R2579W mutant experimental SAS data
Isoform A0B0 of Teneurin-3 R2579W mutant Kratky plot
Sample: Isoform A0B0 of Teneurin-3 R2579W mutant dimer, 533 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2024 Dec 5
Teneurin-3 isoforms, WT and R2579W
RgGuinier 7.7 nm
Dmax 35.0 nm
VolumePorod 1188 nm3

SASDWS7 – Teneurin-3 wildtype A0B1 isoform in 2 mM calcium buffer - 0.48 mg/ml

UniProt ID: Q9WTS6-3 (343-2706) Isoform A0B1 of Teneurin-3 wildtype
Isoform A0B1 of Teneurin-3 wildtype experimental SAS data
Isoform A0B1 of Teneurin-3 wildtype Kratky plot
Sample: Isoform A0B1 of Teneurin-3 wildtype dimer, 535 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2024 Dec 5
Teneurin-3 isoforms, WT and R2579W
Maja Napieraj
RgGuinier 8.2 nm
Dmax 30.5 nm
VolumePorod 1145 nm3

SASDWT7 – Teneurin-3 mutant A0B1 isoform in 2 mM calcium buffer - 1.05 mg/ml

UniProt ID: Q9WTS6-3 (343-2706) Isoform A0B1 of Teneurin-3 mutant
Isoform A0B1 of Teneurin-3 mutant experimental SAS data
Isoform A0B1 of Teneurin-3 mutant Kratky plot
Sample: Isoform A0B1 of Teneurin-3 mutant dimer, 535 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2024 Dec 5
Teneurin-3 isoforms, WT and R2579W
Maja Napieraj
RgGuinier 8.4 nm
Dmax 32.8 nm
VolumePorod 1336 nm3