Browse by MODEL: No model

SASDDW5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum + pyruvate

4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, 5mM sodium pyruvate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.3 nm
Dmax 8.9 nm
VolumePorod 165 nm3

SASDDJ3 – Candida antarctica lipase B - with guanidine-HCl unfolding series

Lipase B from Pseudozyma antarctica experimental SAS data
Lipase B from Pseudozyma antarctica Kratky plot
Sample: Lipase B from Pseudozyma antarctica, 33 kDa Moesziomyces antarcticus protein
Buffer: 100 mM NaCl, 20 mM Na2HPO4, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.4 nm

SASDDK3 – Candida antarctica lipase B - with guanidine-HCl unfolding series, in the presence of dithiothreitol

Lipase B from Pseudozyma antarctica experimental SAS data
Lipase B from Pseudozyma antarctica Kratky plot
Sample: Lipase B from Pseudozyma antarctica, 33 kDa Moesziomyces antarcticus protein
Buffer: 100 mM NaCl, 20 mM Na2HPO4, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.4 nm

SASDDM3 – Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: 10 mM HCl, pH: 1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.3 nm
Dmax 9.0 nm

SASDDN3 – Bovine serum albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

Bovine serum albumin experimental SAS data
Bovine serum albumin Kratky plot
Sample: Bovine serum albumin, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 25
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 117 nm3

SASDDA3 – ATP-dependent Clp protease ATP-binding subunit ClpC1, second state

ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
ATP-dependent Clp protease ATP-binding subunit ClpC1 Kratky plot
Sample: ATP-dependent Clp protease ATP-binding subunit ClpC1, 95 kDa Mycobacterium tuberculosis protein
Buffer: Hepes 50 mM pH 7.5, KCl 100 mM, glycerol 10%, MgCl2 4 mM and ATP 1 mM, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 18
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis. J Biol Chem 293(22):8379-8393 (2018)
Weinhäupl K, Brennich M, Kazmaier U, Lelievre J, Ballell L, Goldberg A, Schanda P, Fraga H
RgGuinier 7.9 nm
Dmax 25.1 nm
VolumePorod 2416 nm3

SASDDT5 – Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)

Nonstructural protein sigma NS20mer RNA (unstructured) experimental SAS data
Nonstructural protein sigma NS 20mer RNA (unstructured) Kratky plot
Sample: Nonstructural protein sigma NS octamer, 325 kDa Avian orthoreovirus protein
20mer RNA (unstructured) dimer, 13 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 25
Stability of local secondary structure determines selectivity of viral RNA chaperones. Nucleic Acids Res (2018)
Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R
RgGuinier 7.8 nm
Dmax 38.0 nm
VolumePorod 964 nm3

SASDDU5 – Nonstructural protein sigma NS - apoprotein

Nonstructural protein sigma NS experimental SAS data
Nonstructural protein sigma NS Kratky plot
Sample: Nonstructural protein sigma NS hexamer, 244 kDa Avian orthoreovirus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 25
Stability of local secondary structure determines selectivity of viral RNA chaperones. Nucleic Acids Res (2018)
Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R
RgGuinier 5.5 nm
Dmax 23.1 nm
VolumePorod 670 nm3

SASDDX4 – Wild-type Protein sex-lethal

Protein sex-lethal experimental SAS data
Protein sex-lethal Kratky plot
Sample: Protein sex-lethal monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 26 nm3

SASDBQ8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus 8mer hairpin dsRNA (SEC-peak2)

Probable ATP-dependent RNA helicase DDX585´ppp 8mer hairpin dsRNA experimental SAS data
Probable ATP-dependent RNA helicase DDX58 5´ppp 8mer hairpin dsRNA Kratky plot
Sample: Probable ATP-dependent RNA helicase DDX58 dimer, 215 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 5.7 nm
Dmax 24.6 nm
VolumePorod 389 nm3