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28 hits found for Structural polyprotein

SASDPY2 – ...structural protein 7-8 (nsp7-8) polyprotein monomer

Replicase polyprotein 1ab experimental SAS data
DAMMIF model
Sample: ...polyprotein 1ab monomer, 31 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
...structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 50 nm3

SASDPZ2 – ...structural protein 7-8 (nsp7-8) polyprotein dimer

Replicase polyprotein 1ab experimental SAS data
Replicase polyprotein 1ab Kratky plot
Sample: ...polyprotein 1ab dimer, 63 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
...structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.3 nm
Dmax 11.8 nm
VolumePorod 110 nm3

SASDP23 – ...structural protein 7-11 (nsp7-11) polyprotein monomer

Replicase polyprotein 1a experimental SAS data
DAMMIF model
Sample: ...polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
...structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.5 nm
Dmax 15.6 nm
VolumePorod 102 nm3

SASDP33 – ...structural protein 7-11 (nsp7-11) polyprotein dimer

Replicase polyprotein 1a experimental SAS data
Replicase polyprotein 1a Kratky plot
Sample: ...polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
...structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 4.6 nm
Dmax 19.1 nm
VolumePorod 203 nm3

SASD2H3 – ...Polyprotein at 8mg/ml

Gag polyprotein experimental SAS data
CORAL model
Sample: ...polyprotein trimer, 150 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris, 500 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, 10 mM beta-mercaproethanol, 1 µM ZnCl2, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 24
...polyprotein in solution. J Struct Biol 218(4):108372 (2026)
Shtykova EV, Petoukhov MV, Dadinova LA, Denieva ZG, Batishchev OV
RgGuinier 4.8 nm
Dmax 18.0 nm
VolumePorod 202 nm3

SASDP56 – ...structural protein 8 (nsp8, dimer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: ...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.1 nm

SASDP66 – ...structural protein 8 (nsp8, dimer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: ...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 98 nm3

SASDP76 – ...structural protein 8 (nsp8, dimer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: ...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 100 nm3

SASDP86 – ...structural protein 8 (nsp8, tetramer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: ...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 6.3 nm
Dmax 24.0 nm

SASDP96 – ...structural protein 8 (nsp8, tetramer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: ...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 5.2 nm
Dmax 18.0 nm

SASDPA6 – ...structural protein 8 (nsp8, tetramer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: ...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.8 nm
Dmax 16.0 nm

SASDXA6 – ...Structural Protein 9 (ORF1ab polyprotein) from SARS CoV-2

ORF1ab polyprotein experimental SAS data
ORF1ab polyprotein Kratky plot
Sample: ...polyprotein dimer, 22 kDa Severe acute respiratory … protein
Buffer: 50mM Tris 300mM Nacl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2025 Jan 20
...Structural Protein Sars CoV-2
Hira Singh Gariya
RgGuinier 3.7 nm
Dmax 6.9 nm
VolumePorod 66 nm3

SASDXB6 – ...Structural Protein 15 (Replicase polyprotein 1ab) from SARS Cov-2

Non structural Protein 15 experimental SAS data
Non structural Protein 15 Kratky plot
Sample: ...structural Protein 15 hexamer, 228 kDa Severe acute respiratory … protein
Buffer: 20mM HEPES, 150mM Nacl, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2024 Jun 13
...Structural Protein Sars CoV-2
Hira Singh Gariya
RgGuinier 4.5 nm
Dmax 13.7 nm
VolumePorod 392 nm3

SASD2E6 – ...Polyprotein at 2 mg/ml

Gag polyprotein experimental SAS data
Human immunodeficiency virus type 1 (HIV-1) Gag Polyprotein at 2 mg/ml Rg histogram
Sample: ...polyprotein monomer, 50 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris, 500 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, 10 mM beta-mercaproethanol, 1 µM ZnCl2, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 24
...polyprotein in solution. J Struct Biol 218(4):108372 (2026)
Shtykova EV, Petoukhov MV, Dadinova LA, Denieva ZG, Batishchev OV
RgGuinier 4.1 nm
Dmax 16.0 nm
VolumePorod 64 nm3

SASDUS6 – Amino-terminal domain of Infectious Bursal Disease Virus VP3 at pH8.0

Structural polyprotein experimental SAS data
Structural polyprotein Kratky plot
Sample: Structural polyprotein dimer, 26 kDa Infectious bursal disease … protein
Buffer: 50 mM Tris, 500 mM NaCl, 2 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 27
Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role. PNAS Nexus 3(12):pgae521 (2024)
Ferrero DS, Gimenez MC, Sagar A, Rodríguez JM, Castón JR, Terebiznik MR, Bernadó P, Verdaguer N
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 52 nm3

SASDKT6 – ...structural protein 14 (nsp14)

Replicase polyprotein 1ab (non-structural protein 14) experimental SAS data
PYMOL model
Sample: ...polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.7 nm
Dmax 9.6 nm
VolumePorod 80 nm3

SASDKU6 – ...structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10) experimental SAS data
GASBOR model
Sample: ...polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
...polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.9 nm
Dmax 12.2 nm
VolumePorod 73 nm3

SASDKV6 – ...structural protein 10/non-structural protein 16 complex (nsp10/nsp16)

Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: ...polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
...polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.0 nm
Dmax 8.0 nm
VolumePorod 28 nm3

SASDKW6 – ...structural protein 10/non-structural protein 14/non-structural protein 16 triplex (nsp10/nsp14/nsp16)

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: ...polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
...polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
...polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 4.7 nm
Dmax 11.4 nm
VolumePorod 113 nm3

SASDUR7 – Infectious Bursal Disease Virus VP3 (central domains at pH 8.0)

Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) experimental SAS data
Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) Kratky plot
Sample: Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) monomer, 18 kDa Infectious bursal disease … protein
Buffer: 50 mM TRIS, 500 mM NaCl, 2 mM DTT,, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role. PNAS Nexus 3(12):pgae521 (2024)
Ferrero DS, Gimenez MC, Sagar A, Rodríguez JM, Castón JR, Terebiznik MR, Bernadó P, Verdaguer N
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 21 nm3

SASDUS7 – Infectious Bursal Disease Virus VP3 (C-terminal deleted at pH 8.0)

Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) experimental SAS data
Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) Kratky plot
Sample: Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) dimer, 55 kDa Infectious bursal disease … protein
Buffer: 50 mM TRIS, 500 mM NaCl, 2 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 1
Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role. PNAS Nexus 3(12):pgae521 (2024)
Ferrero DS, Gimenez MC, Sagar A, Rodríguez JM, Castón JR, Terebiznik MR, Bernadó P, Verdaguer N
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 110 nm3

SASDVV8 – nsp14 and nsp10 from SARS-CoV-2

Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14)Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
DAMMIF model
Sample: ...polyprotein 1ab (Guanine-N7 methyltransferase nsp14) monomer, 60 kDa Severe acute respiratory … protein
...polyprotein 1ab (Non-structural protein 10) monomer, 17 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Feb 17
A SEC-SAXS analysis of SARS-CoV-2 nsp14 apo or in complex with vhh and/or nsp10
Pierre Gauffre
RgGuinier 3.2 nm
Dmax 12.3 nm
VolumePorod 85 nm3

SASDVW8 – nsp14 and nsp10 from SARS-CoV-2 with vhh

Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14)Replicase polyprotein 1ab (Non-structural protein 10)vhh anti-nsp14 SARS-CoV-2 experimental SAS data
Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14) Replicase polyprotein 1ab (Non-structural protein 10) vhh anti-nsp14 SARS-CoV-2 Kratky plot
Sample: ...polyprotein 1ab (Guanine-N7 methyltransferase nsp14) monomer, 60 kDa Severe acute respiratory … protein
...polyprotein 1ab (Non-structural protein 10) monomer, 17 kDa Severe acute respiratory … protein
vhh anti-nsp14 SARS-CoV-2 monomer, 14 kDa protein
Buffer: 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 17
A SEC-SAXS analysis of SARS-CoV-2 nsp14 apo or in complex with vhh and/or nsp10
Pierre Gauffre
RgGuinier 3.8 nm
Dmax 19.7 nm
VolumePorod 147 nm3

SASDS69 – ...structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: ...polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
...Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 21 nm3

SASDS79 – ...structural protein 10 (Nsp10: C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) Kratky plot
Sample: ...polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
...Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.6 nm
Dmax 5.4 nm
VolumePorod 19 nm3

SASDS89 – ...structural protein 10 (Nsp10: N- and C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) Kratky plot
Sample: ...polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
...Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.5 nm
Dmax 5.1 nm
VolumePorod 16 nm3

SASDS99 – ...structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: ...polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
...Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 19 nm3

SASDSA9 – ...structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: ...polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
...Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 19 nm3