SASBDB entries for UniProt ID:

SASDAP7 – PBX1-PREB1 complex

UniProt ID: P40424 (1-308) Pre-B-cell leukemia transcription factor 1

UniProt ID: P55347 (1-344) Homeobox protein PKNOX1
Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1 experimental SAS data
DAMMIF model
Sample: Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein
Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding. FEBS J 283(16):3134-54 (2016)
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
RgGuinier 5.8 nm
Dmax 19.0 nm
VolumePorod 143 nm3

SASDAQ7 – PBX1-PREB1-DNA complex

UniProt ID: P40424 (1-308) Pre-B-cell leukemia transcription factor 1

UniProt ID: P55347 (1-344) Homeobox protein PKNOX1

UniProt ID: (None-None) DNA 22mer
Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1DNA 22mer experimental SAS data
DAMMIF model
Sample: Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein
Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein
DNA 22mer monomer, 12 kDa DNA
Buffer: 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding. FEBS J 283(16):3134-54 (2016)
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
RgGuinier 4.8 nm
Dmax 16.3 nm
VolumePorod 173 nm3

SASDAS7 – mouse olfactomedin-1

UniProt ID: O88998 (None-None) Noelin
Noelin experimental SAS data
DAMMIF model
Sample: Noelin tetramer, 256 kDa Mus musculus protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 6
Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure. J Biol Chem 290(24):15092-101 (2015)
Pronker MF, Bos TG, Sharp TH, Thies-Weesie DM, Janssen BJ
RgGuinier 8.5 nm
Dmax 30.0 nm
VolumePorod 616 nm3

SASDAY7 – IL-6R tetramer-dimer equilibrium

UniProt ID: P08887 (None-None) Interleukin-6 receptor subunit alpha

UniProt ID: P08887 (None-None) Interleukin-6 receptor subunit alpha
Interleukin-6 receptor subunit alphaInterleukin-6 receptor subunit alpha experimental SAS data
CORAL model
Sample: Interleukin-6 receptor subunit alpha tetramer, 164 kDa Homo sapiens protein
Interleukin-6 receptor subunit alpha dimer, 82 kDa Homo sapiens protein
Buffer: water, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2014 Oct 1
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 4.9 nm
Dmax 20.0 nm
VolumePorod 240 nm3

SASDAZ7 – IL-6R AIR-3A 2:4 complex

UniProt ID: (None-None) AIR-3A

UniProt ID: P08887 (None-None) Interleukin-6 receptor subunit alpha
AIR-3AInterleukin-6 receptor subunit alpha experimental SAS data
DAMMIF model
Sample: AIR-3A tetramer, 26 kDa RNA
Interleukin-6 receptor subunit alpha dimer, 82 kDa Homo sapiens protein
Buffer: water, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2014 Oct 1
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 6.7 nm
Dmax 25.0 nm
VolumePorod 150 nm3

SASDA58 – UL26N of pseudorabies virus

UniProt ID: Q83417 (1-224) VP24
VP24 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: VP24 dimer, 53 kDa Suid herpesvirus 1 protein
Buffer: 50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease. PLoS Pathog 11(7):e1005045 (2015)
Zühlsdorf M, Werten S, Klupp BG, Palm GJ, Mettenleiter TC, Hinrichs W
RgGuinier 2.6 nm
Dmax 10.6 nm
VolumePorod 73 nm3

SASDAB8 – Protein Interacting with C-kinase 1 (PICK1) LKV, dimer contribution (data decomposition).

UniProt ID: Q9EP80 (2-416) PRKCA-binding protein
PRKCA-binding protein experimental SAS data
Protein Interacting with C-kinase 1 (PICK1) LKV, dimer contribution (data decomposition). Rg histogram
Sample: PRKCA-binding protein dimer, 93 kDa Rattus norvegicus protein
Buffer: 50 mM Tris 125 mM NaCl 0.01 vol% reduced TX-100, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2015 May 11
Structure of Dimeric and Tetrameric Complexes of the BAR Domain Protein PICK1 Determined by Small-Angle X-Ray Scattering. Structure 23(7):1258-1270 (2015)
Karlsen ML, Thorsen TS, Johner N, Ammendrup-Johnsen I, Erlendsson S, Tian X, Simonsen JB, Høiberg-Nielsen R, Christensen NM, Khelashvili G, Streicher W, Teilum K, Vestergaard B, Weinstein H, Gether U, Arleth L, Madsen KL
RgGuinier 6.0 nm
Dmax 20.0 nm
VolumePorod 205 nm3

SASDAC8 – SDS hydrolase SdsA1

UniProt ID: Q9I5I9 (None-None) Pseudomonas aeruginosa SDS hydrolase SdsA1
Pseudomonas aeruginosa SDS hydrolase SdsA1 experimental SAS data
CRYSOL model
Sample: Pseudomonas aeruginosa SDS hydrolase SdsA1 dimer, 145 kDa Pseudomonas aeruginosa protein
Buffer: 50 mM HEPES, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Jul 22
SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques Journal of Applied Crystallography 48(5):1551-1559 (2015)
De la Mora E, Flores-Hernández E, Jakoncic J, Stojanoff V, Siliqi D, Sánchez-Puig N, Moreno A
RgGuinier 3.6 nm
Dmax 16.9 nm
VolumePorod 261 nm3

SASDAD8 – Antiapoptotic membrane protein, (DpV84gp022) Deerpox virus

UniProt ID: Q08FF8 (1-155) Antiapoptotic membrane protein
Antiapoptotic membrane protein experimental SAS data
Antiapoptotic membrane protein Kratky plot
Sample: Antiapoptotic membrane protein dimer, 39 kDa Deerpox virus W-1170-84 protein
Buffer: 25 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2013 May 4
Structural basis of Deerpox virus-mediated inhibition of apoptosis. Acta Crystallogr D Biol Crystallogr 71(Pt 8):1593-603 (2015)
Burton DR, Caria S, Marshall B, Barry M, Kvansakul M
RgGuinier 2.6 nm
Dmax 11.1 nm
VolumePorod 61 nm3

SASDAE8 – Human Filamin A Ig-like domains 20-21 truncation (2151-2329) in complex with migfilin peptide

UniProt ID: P21333 (2151-2329) Human Filamin A Ig-like domains 20-21/migfilin peptide complex
Human Filamin A Ig-like domains 20-21/migfilin peptide complex experimental SAS data
Human Filamin A Ig-like domains 20-21/migfilin peptide complex Kratky plot
Sample: Human Filamin A Ig-like domains 20-21/migfilin peptide complex monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM Tris 50 mM NaCl 10mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Feb 1
Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21. PLoS One 10(8):e0136969 (2015)
Seppälä J, Tossavainen H, Rodic N, Permi P, Pentikäinen U, Ylänne J
RgGuinier 2.4 nm
Dmax 8.2 nm
VolumePorod 31 nm3