Small Angle Scattering Biological Data Bank SASBDB


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42 hits found for Sarasi Galagedera

SASDS96 – Human Ubiquilin-1 (UBQLN1)

Sample:	Ubiquilin-1 dimer, 125 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
...Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	4.6	nm
D_max	19.3	nm
Volume^Porod	264	nm³

SASDSA6 – Human Ubiquilin-2 (UBQLN2)

Sample:	Ubiquilin-2 dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
...Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	5.2	nm
D_max	22.5	nm
Volume^Porod	330	nm³

SASDSB6 – Human Ubiquilin-4 (UBQLN4)

Sample:	Ubiquilin-4 dimer, 128 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 26

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
...Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	5.1	nm
D_max	21.7	nm
Volume^Porod	333	nm³

SASDSC6 – Human Ubiquilin-2, ∆STI1-1 domain deletion (UBQLN2∆STI1-1)

Ubiquilin-2 (∆170-260) experimental SAS data

Sample:	Ubiquilin-2 (∆170-260) dimer, 110 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.4	nm
D_max	26.0	nm
Volume^Porod	357	nm³

SASDSD6 – Human Ubiquilin-2 with ∆UBL and ∆STI1-1 domain deletions (UBQLN2∆STI1-1∆UBL)

Ubiquilin-2 (∆33-103, ∆170-260) experimental SAS data

Ubiquilin-2 (∆33-103, ∆170-260) Kratky plot

Sample:	Ubiquilin-2 (∆33-103, ∆170-260) dimer, 94 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.6	nm
D_max	24.5	nm
Volume^Porod	326	nm³

SASDSE6 – Human Ubiquilin-2, ∆1-108 N-terminal deletion (UBQLN2 109-624)

Ubiquilin-2 (∆1-108) experimental SAS data

Sample:	Ubiquilin-2 (∆1-108) dimer, 108 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.2	nm
D_max	23.3	nm
Volume^Porod	281	nm³

SASDSF6 – Human Ubiquilin-2, C-terminal construct (UBQLN2 450-624W)

Ubiquilin-2 (∆1-449)-W experimental SAS data

Sample:	Ubiquilin-2 (∆1-449)-W dimer, 35 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	3.8	nm
D_max	18.5	nm
Volume^Porod	50	nm³

SASDSG6 – Human Ubiquilin-2, ∆STI1-2 domain deletion (UBQLN2∆STI1-2)

Ubiquilin-2 (∆379-462) experimental SAS data

Sample:	Ubiquilin-2 (∆379-462) monomer, 56 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera

R_g^Guinier	4.4	nm
D_max	22.4	nm
Volume^Porod	121	nm³

SASDSH6 – Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX)

Ubiquilin-2 (∆487-538) experimental SAS data

Sample:	Ubiquilin-2 (∆487-538) dimer, 122 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera

R_g^Guinier	4.8	nm
D_max	19.5	nm
Volume^Porod	288	nm³

SASDSJ6 – Human Ubiquilin-2, P497E phosphomimetic point mutant (UBQLN2 P497E)

Ubiquilin-2 (P497E) experimental SAS data

Sample:	Ubiquilin-2 (P497E) dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.3	nm
D_max	21.3	nm
Volume^Porod	340	nm³

SASDSK6 – Human Ubiquilin-2, P497L ALS-linked point mutant (UBQLN2 P497L)

Ubiquilin-2 (P497L) experimental SAS data

Sample:	Ubiquilin-2 (P497L) dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	7.3	nm
D_max	28.8	nm
Volume^Porod	1365	nm³

SASDSL6 – Human Ubiquilin-2, R12G R15D Q21E triple point mutant (UBQLN2 GDE)

Ubiquilin-2 (R12G, R15D, Q21E) experimental SAS data

Ubiquilin-2 (R12G, R15D, Q21E) Kratky plot

Sample:	Ubiquilin-2 (R12G, R15D, Q21E) dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.1	nm
D_max	21.3	nm
Volume^Porod	330	nm³

SASDSM6 – Human Ubiquilin-2, ∆551-569 deletion construct (UBQLN2∆551-569)

Ubiquilin-2 (∆551-569) experimental SAS data

Sample:	Ubiquilin-2 (∆551-569) dimer, 127 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.2	nm
D_max	23.4	nm
Volume^Porod	332	nm³

SASDSN6 – Human full length RAD23B

UV excision repair protein RAD23 homolog B experimental SAS data

UV excision repair protein RAD23 homolog B Kratky plot

Sample:	UV excision repair protein RAD23 homolog B monomer, 43 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22

Domain deletion constructs of hHR23B
Sarasi Galagedera

R_g^Guinier	4.6	nm
D_max	26.0	nm
Volume^Porod	103	nm³

SASDSP6 – Human RAD23B, ∆UBL domain deletion (RAD23B∆UBL)

UV excision repair protein RAD23 homolog B (∆1-79) experimental SAS data

UV excision repair protein RAD23 homolog B (∆1-79) Kratky plot

Sample:	UV excision repair protein RAD23 homolog B (∆1-79) monomer, 35 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22

Domain deletion constructs of hHR23B
Sarasi Galagedera

R_g^Guinier	4.6	nm
D_max	21.6	nm
Volume^Porod	92	nm³

SASDSQ6 – Human RAD23B, ∆UBA-1 domain deletion (RAD23B∆UBA1)

UV excision repair protein RAD23 homolog B (∆186-231) experimental SAS data

UV excision repair protein RAD23 homolog B (∆186-231) Kratky plot

Sample:	UV excision repair protein RAD23 homolog B (∆186-231) monomer, 38 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22

Domain deletion constructs of hHR23B
Sarasi Galagedera

R_g^Guinier	4.6	nm
D_max	21.7	nm
Volume^Porod	95	nm³

SASDSR6 – Human RAD23B, ∆UBA-2 domain deletion (RAD23B∆UBA2)

UV excision repair protein RAD23 homolog B (∆362-409) experimental SAS data

UV excision repair protein RAD23 homolog B (∆362-409) Kratky plot

Sample:	UV excision repair protein RAD23 homolog B (∆362-409) monomer, 38 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22

Domain deletion constructs of hHR23B
Sarasi Galagedera

R_g^Guinier	4.1	nm
D_max	17.5	nm
Volume^Porod	79	nm³

SASDSS6 – Human RAD23B, ∆STI1 domain deletion (RAD23B∆STI1)

UV excision repair protein RAD23 homolog B (∆274-306) experimental SAS data

UV excision repair protein RAD23 homolog B (∆274-306) Kratky plot

Sample:	UV excision repair protein RAD23 homolog B (∆274-306) monomer, 39 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22

Domain deletion constructs of hHR23B
Sarasi Galagedera

R_g^Guinier	4.6	nm
D_max	24.2	nm
Volume^Porod	103	nm³

SASDST6 – Human RAD23B with ∆UBA-1 and ∆UBA-2 domain deletions (RAD23B∆UBA1∆UBA2)

UV excision repair protein RAD23 homolog B (∆186-231, ∆362-409) experimental SAS data

UV excision repair protein RAD23 homolog B (∆186-231, ∆362-409) Kratky plot

Sample:	UV excision repair protein RAD23 homolog B (∆186-231, ∆362-409) monomer, 33 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22

Domain deletion constructs of hHR23B
Sarasi Galagedera

R_g^Guinier	4.9	nm
D_max	25.5	nm
Volume^Porod	100	nm³

SASDR37 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin

HOTag-GS-Ubiquitin experimental SAS data

Sample:	HOTag-GS-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C

R_g^Guinier	3.7	nm
D_max	14.0	nm
Volume^Porod	74	nm³

SASDR47 – HOTag6 tetramerization domain followed by a (Gly-Ser)2 linker fused to human monoubiquitin

HOTag6-(GS)2-Ubiquitin experimental SAS data

Sample:	HOTag6-(GS)2-Ubiquitin tetramer, 52 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21

R_g^Guinier	3.7	nm
D_max	13.7	nm
Volume^Porod	69	nm³

SASDR57 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin

HOTag-(GS)4-Ubiquitin experimental SAS data

Sample:	HOTag-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

R_g^Guinier	3.8	nm
D_max	14.3	nm
Volume^Porod	76	nm³

SASDR67 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin

HOTag-(GS)10-Ubiquitin experimental SAS data

Sample:	HOTag-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

R_g^Guinier	4.0	nm
D_max	14.8	nm
Volume^Porod	84	nm³

SASDR77 – HOTag6 tetramerization domain followed by a (Gly-Ser)25 linker fused to human monoubiquitin

HOTag6-(GS)25-Ubiquitin experimental SAS data

Sample:	HOTag6-(GS)25-Ubiquitin tetramer, 65 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21

R_g^Guinier	4.7	nm
D_max	19.3	nm
Volume^Porod	117	nm³

SASDR87 – HOTag6 tetramerization domain followed by a (Gly-Ser)50 linker fused to human monoubiquitin

HOTag-(GS)50-Ubiquitin experimental SAS data

Sample:	HOTag-(GS)50-Ubiquitin tetramer, 80 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

R_g^Guinier	5.6	nm
D_max	22.7	nm
Volume^Porod	193	nm³

SASDR97 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin

HOTag-PA-Ubiquitin experimental SAS data

Sample:	HOTag-PA-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

R_g^Guinier	3.7	nm
D_max	12.9	nm
Volume^Porod	73	nm³

SASDRA7 – HOTag6 tetramerization domain followed by a (Pro-Ala)2 linker fused to human monoubiquitin

HOTag6-(PA)2-Ubiquitin experimental SAS data

Sample:	HOTag6-(PA)2-Ubiquitin tetramer, 52 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28

R_g^Guinier	3.8	nm
D_max	15.0	nm
Volume^Porod	74	nm³

SASDRB7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin

HOTag-(PA)4-Ubiquitin experimental SAS data

Sample:	HOTag-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

R_g^Guinier	4.1	nm
D_max	14.4	nm
Volume^Porod	78	nm³

SASDRC7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data

Sample:	HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

R_g^Guinier	5.1	nm
D_max	18.4	nm
Volume^Porod	106	nm³

SASDRD7 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)-Ubiquitin experimental SAS data

Sample:	HOTag6-(GS)-Ubiquitin tetramer, 51 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19

R_g^Guinier	3.6	nm
D_max	12.6	nm
Volume^Porod	70	nm³

SASDRE7 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)4-Ubiquitin experimental SAS data

Sample:	HOTag6-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19

R_g^Guinier	3.8	nm
D_max	12.8	nm
Volume^Porod	114	nm³

SASDRF7 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)10-Ubiquitin experimental SAS data

Sample:	HOTag6-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19

R_g^Guinier	4.1	nm
D_max	14.2	nm
Volume^Porod	86	nm³

SASDRG7 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)-Ubiquitin experimental SAS data

Sample:	HOTag6-(PA)-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19

R_g^Guinier	3.7	nm
D_max	12.4	nm
Volume^Porod	71	nm³

SASDRH7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)4-Ubiquitin experimental SAS data

Sample:	HOTag6-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19

R_g^Guinier	4.1	nm
D_max	14.7	nm
Volume^Porod	80	nm³

SASDRJ7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)10-Ubiquitin experimental SAS data

Sample:	HOTag6-(PA)10-Ubiquitin tetramer, 57 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19

R_g^Guinier	5.1	nm
D_max	19.0	nm
Volume^Porod	105	nm³

SASDRK7 – M1-linked tetraubiquitin, M1(1-72)-Ub4

Sample:	Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28

R_g^Guinier	3.2	nm
D_max	12.5	nm
Volume^Porod	41	nm³

SASDRL7 – M1-linked tetraubiquitin, M1(1-73)-Ub4

Sample:	Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28

R_g^Guinier	3.3	nm
D_max	12.8	nm
Volume^Porod	41	nm³

SASDRM7 – M1-linked tetraubiquitin, M1(1-74)-Ub4

Sample:	Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28

R_g^Guinier	3.2	nm
D_max	12.2	nm
Volume^Porod	43	nm³

SASDRN7 – M1-linked tetraubiquitin Pro-Ser/Gly-Ser fusion, M1(1-76)-PS(GS)4-Ub4

Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 experimental SAS data

Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 Kratky plot

Sample:	Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 monomer, 36 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28

R_g^Guinier	3.4	nm
D_max	13.9	nm
Volume^Porod	50	nm³

SASDRP7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)3A-Ub4

Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub experimental SAS data

Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub Kratky plot

Sample:	Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub monomer, 39 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28

R_g^Guinier	4.2	nm
D_max	18.2	nm
Volume^Porod	62	nm³

SASDRQ7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)6A-Ub4

Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub experimental SAS data

Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub Kratky plot

Sample:	Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub monomer, 43 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28

R_g^Guinier	5.2	nm
D_max	21.2	nm
Volume^Porod	108	nm³

SASDRK8 – HOTag6 tetramerization domain followed by a (Pro-Ala)25 linker fused to human monoubiquitin

Sample:	HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22

R_g^Guinier	6.3	nm
D_max	24.9	nm
Volume^Porod	180	nm³

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