Small Angle Scattering Biological Data Bank SASBDB


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63 hits found for Albumin

SASDF22 – ...albumin and the human growth hormone derivative somapacitan.

somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data

Sample:	somapacitan monomer, 22 kDa Homo sapiens protein ...albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer:	100 mM MES, 100 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at I911-4, MAX IV on 2015 May 11

...albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P

R_g^Guinier	4.2	nm
D_max	14.7	nm
Volume^Porod	227	nm³

SASDA32 – ...albumin in HEPES

Bovine Serum Albumin experimental SAS data

Sample:	...Albumin monomer, 69 kDa Bos taurus (Bovine) protein
Buffer:	50 mM HEPES 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 12

Standard proteins
Darja Ruskule

R_g^Guinier	2.9	nm
D_max	9.3	nm
Volume^Porod	97	nm³

SASDF32 – ...albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data

Sample:	...albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein Albumin-binding side-chain monomer, 1 kDa neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer:	100 mM MES, 100 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at I911-4, MAX IV on 2015 Nov 11

R_g^Guinier	3.6	nm
D_max	12.6	nm
Volume^Porod	174	nm³

SASDF42 – ...albumin in complex with somapacitan

Human serum albuminSomapacitan experimental SAS data

Sample:	...albumin monomer, 66 kDa Homo sapiens protein Somapacitan dimer, 44 kDa Homo sapiens protein
Buffer:	100 mM MES, 140 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2015 Sep 4

R_g^Guinier	4.1	nm
D_max	13.9	nm
Volume^Porod	202	nm³

SASDPH3 – Benchmarking extraction methods of scattering patterns of poorly resolved components from a SEC-SAXS dataset of BSA.

Sample:	...Albumin trimer, 199 kDa Bos taurus protein
Buffer:	20 mM Hepes, 150mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2021 Sep 24

SEC-SAXS: Experimental set-up and software developments build up a powerful tool. Methods Enzymol 677:221-249 (2022)
Pérez J, Thureau A, Vachette P

R_g^Guinier	5.2	nm
D_max	17.6	nm
Volume^Porod	370	nm³

SASDBJ3 – ...albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data

Sample:	...albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI

R_g^Guinier	2.8	nm
D_max	8.2	nm
Volume^Porod	100	nm³

SASDCJ3 – ...Albumin

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...

R_g^Guinier	2.8	nm
D_max	8.7	nm
Volume^Porod	101	nm³

SASDBK3 – ...albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data

Sample:	...albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

R_g^Guinier	3.9	nm
D_max	12.7	nm
Volume^Porod	202	nm³

SASDDN3 – ...albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

Sample:	...albumin , 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Sep 25

Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	117	nm³

SASDBT4 – ...albumin monomer (measured by SEC-SAXS)

Sample:	...albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer:	20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2016 Feb 22

...Albumin measured by SEC-SAXS
Martha Brennich

R_g^Guinier	2.7	nm
D_max	8.2	nm
Volume^Porod	118	nm³

SASDE65 – ...albumin monomer from in-house SEC-SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B

R_g^Guinier	2.8	nm
D_max	9.2	nm
Volume^Porod	92	nm³

SASDE75 – ...albumin monomer from in-house SEC-SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	2.8	nm
D_max	9.9	nm
Volume^Porod	104	nm³

SASDE85 – ...albumin monomer from in-house SEC-SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	2.8	nm
D_max	8.2	nm
Volume^Porod	85	nm³

SASDE95 – ...albumin monomer from in-house SEC-SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	2.9	nm
D_max	8.6	nm
Volume^Porod	74	nm³

SASDEA5 – ...albumin monomer from in-house SEC-SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 25

R_g^Guinier	2.8	nm
D_max	9.6	nm
Volume^Porod	78	nm³

SASDEB5 – ...albumin from in-house SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 May 4

R_g^Guinier	2.8	nm
D_max	9.4	nm
Volume^Porod	99	nm³

SASDEC5 – ...albumin dimer from in-house SEC-SAXS

Sample:	...albumin dimer, 133 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	4.1	nm
D_max	13.8	nm
Volume^Porod	200	nm³

SASDED5 – ...albumin monomer from synchrotron SEC-SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS + 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 14

R_g^Guinier	2.7	nm
D_max	8.7	nm
Volume^Porod	84	nm³

SASDEE5 – ...albumin from synchrotron SAXS

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	85	nm³

SASDAA6 – ...albumin monomer and mixtures

Human serum albumin monomer experimental SAS data

Sample:	...albumin monomer monomer, 66 kDa Homo sapiens protein
Buffer:	50 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22

Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI

R_g^Guinier	2.8	nm
D_max	8.4	nm
Volume^Porod	103	nm³

SASDP67 – ...albumin at neutral pH, 4 °C, and 5 MPa (1 atm) of pressure

Sample:	Albumin monomer, 69 kDa Bos taurus protein
Buffer:	25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	2.7	nm
D_max	8.5	nm
Volume^Porod	99	nm³

SASDP77 – ...albumin at neutral pH, 4 °C, and 100 MPa (1000 atm) of pressure

Sample:	Albumin monomer, 69 kDa Bos taurus protein
Buffer:	25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	2.6	nm
D_max	8.3	nm
Volume^Porod	94	nm³

SASDP87 – ...albumin at neutral pH, 50 °C, and 5 MPa (1 atm) of pressure

Sample:	Albumin monomer, 69 kDa Bos taurus protein
Buffer:	25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	3.0	nm
D_max	9.5	nm
Volume^Porod	108	nm³

SASDP97 – ...albumin at neutral pH, 50 °C, and 100 MPa (1000 atm) of pressure

Sample:	Albumin monomer, 69 kDa Bos taurus protein
Buffer:	25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	2.8	nm
D_max	9.4	nm
Volume^Porod	104	nm³

SASDCF8 – ...albumin (WAXS)

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	Hepes, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 25

WAXS benchmark on standard proteins
Maxim Petoukhov

R_g^Guinier

2.7

SASDRL8 – ...albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Jun 7

NSRRC TPS13A standard protein archive
Orion Shih

R_g^Guinier	2.9	nm
D_max	8.9	nm
Volume^Porod	84	nm³

SASDCQ8 – ...albumin (BSA)

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	TRIS 50mM, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21

A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet

R_g^Guinier

2.8

SASDRQ8 – ...albumin monomer separated using asymmetric flow field flow fractionation (AFFFF)

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jun 8

Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	104	nm³

SASDHV8 – ...albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.0

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein ...albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21

Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P

R_g^Guinier	4.9	nm
D_max	16.0	nm
Volume^Porod	267	nm³

SASDHW8 – ...albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein ...albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 5.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21

R_g^Guinier	4.9	nm
D_max	16.0	nm
Volume^Porod	274	nm³

SASDHX8 – ...albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 6.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein ...albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7

R_g^Guinier	4.6	nm
D_max	16.0	nm
Volume^Porod	258	nm³

SASDQX8 – ...albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...

R_g^Guinier

3.1

SASDHY8 – ...albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 7.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein ...albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 26

R_g^Guinier	5.0	nm
D_max	17.4	nm
Volume^Porod	270	nm³

SASDQY8 – ...albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 15

R_g^Guinier

2.7

SASDHZ8 – ...albumin/Neprilysin fusion protein (HSA-NEP): 10 mM TRIS, pH 8.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein ...albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM TRIS, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Dec 15

R_g^Guinier	4.9	nm
D_max	16.7	nm
Volume^Porod	239	nm³

SASDH29 – ...albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein ...albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM phosphate, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7

R_g^Guinier	4.9	nm
D_max	16.7	nm
Volume^Porod	240	nm³

SASDLC4 – ...Albumin (HSA) Healthy donor used for HNA1, HNA2, HMA fractions

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	8.5	nm

SASDEV5 – Insulin detemir tri-hexamer

Sample:	Insulin detemir 18-mer, 106 kDa protein
Buffer:	5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at I911-4, MAX IV on 2015 Sep 25

...albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P

R_g^Guinier	3.3	nm
D_max	11.3	nm
Volume^Porod	137	nm³

SASDL24 – ...Albumin (HMA)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	9.2	nm

SASDEW5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site II

Insulin detemir (Levemir(R), Novo Nordisk A/S)Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) experimental SAS data

Sample:	Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein ...Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer:	6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at I911-4, MAX IV on 2015 Sep 25

...albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P

R_g^Guinier	3.5	nm
D_max	13.0	nm
Volume^Porod	162	nm³

SASDFQ8 – ...albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	98	nm³

SASDL34 – ...Albumin (HNA1)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.9	nm
D_max	8.9	nm

SASDEX5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site I

Sample:	Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein ...Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer:	6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at I911-4, MAX IV on 2015 Sep 25

...albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P

R_g^Guinier	3.5	nm
D_max	13.0	nm
Volume^Porod	162	nm³

SASDFR8 – ...albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	...albumin dimer, 133 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	4.0	nm
D_max	13.2	nm
Volume^Porod	211	nm³

SASDL44 – ...Albumin (HNA2)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	8.1	nm

SASDEZ5 – Albumin-insulin detemir 2:12 complex, P2 symmetry

Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)Insulin detemir (Levemir(R), Novo Nordisk A/S) experimental SAS data

Sample:	...Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein
Buffer:	8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at I911-4, MAX IV on 2015 Sep 25

...albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P

R_g^Guinier	5.4	nm
D_max	19.5	nm
Volume^Porod	309	nm³

SASDLU3 – ...Albumin (C1)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.7	nm
D_max	8.2	nm

SASDEY5 – Albumin-insulin detemir 2:12 complex, P1 symmetry

Sample:	...Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein
Buffer:	8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at I911-4, MAX IV on 2015 Sep 25

...albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P

R_g^Guinier	5.4	nm
D_max	19.5	nm
Volume^Porod	309	nm³

SASDLV3 – ...Albumin (C2)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	8.3	nm

SASDE26 – Albumin-insulin degludec 1:12 complex

Human Albumin (Recombumin(R) Elite, Albumedix Ltd.)Insulin degludec(Tresiba(R), Novo Nordisk A/S) experimental SAS data

Sample:	...Albumin (Recombumin(R) Elite, Albumedix Ltd.) monomer, 66 kDa protein Insulin degludec(Tresiba(R), Novo Nordisk A/S) dodecamer, 73 kDa protein
Buffer:	25 mM Na2HPO4, 15.9 mM m-cresol, 15.9 mM phenol, 212.8 mM glycerol, 20 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 27

...albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P

R_g^Guinier	3.8	nm
D_max	13.4	nm
Volume^Porod	179	nm³

SASDF99 – ...albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	...albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23

R_g^Guinier	2.8	nm
D_max	8.3	nm
Volume^Porod	95	nm³

SASDLW3 – ...Albumin (C3)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	8.3	nm

SASDLX3 – ...Albumin (C4)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	8.4	nm

SASDLY3 – ...Albumin (C6) control SIGMA

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier

2.8

SASDLZ3 – ...Albumin (C6) control SIGMA

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier

2.8

SASDL54 – ...Albumin (P1)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.9	nm
D_max	9.3	nm

SASDL64 – ...Albumin (P2)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.9	nm
D_max	9.4	nm

SASDL74 – ...Albumin (P3) patient

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.9	nm
D_max	8.7	nm

SASDL84 – ...Albumin (P4)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.9	nm
D_max	9.0	nm

SASDL94 – ...Albumin (P5)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.9	nm
D_max	8.9	nm

SASDLA4 – ...Albumin (P6)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	8.5	nm

SASDLD4 – ...Albumin (P7)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	9.2	nm

SASDLE4 – ...Albumin (P9)

Sample:	Albumin monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1

Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M

R_g^Guinier	2.8	nm
D_max	8.9	nm

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