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191 hits found for Pollack

SASDCE4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 3000ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.2 nm
Dmax 6.7 nm

SASDRE4 – GAC rRNA + L11 Protein: Time-Resolved 316 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 38 nm3

SASDTE4 – Tissue Transglutaminase + 100 µM inhibitor 5826 (2% DMSO)

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 3.8 nm
Dmax 17.6 nm
VolumePorod 130 nm3

SASDCF4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 20mM KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.6 nm
Dmax 8.5 nm

SASDRF4 – GAC rRNA + L11 Protein: Time-Resolved 631 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.5 nm
Dmax 13.0 nm
VolumePorod 38 nm3

SASDTF4 – Tissue Transglutaminase + CaCl2 + inhibitor 5826: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM 5826 inhibitor, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 6.7 nm
Dmax 39.0 nm
VolumePorod 450 nm3

SASDCG4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 100mM KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 100mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.5 nm
Dmax 8.0 nm

SASDRG4 – GAC rRNA + L11 Protein: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.5 nm
Dmax 12.5 nm
VolumePorod 40 nm3

SASDTG4 – Tissue Transglutaminase + CaCl2 + inhibitor 5826 + GTP: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM 5826, 5 mM GTP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 6.0 nm
Dmax 29.0 nm
VolumePorod 248 nm3

SASDCH4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 200mm KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 200mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.5 nm
Dmax 8.0 nm

SASDRH4 – GAC rRNA + L11 Protein: Time-Resolved 2000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.6 nm
Dmax 12.8 nm
VolumePorod 39 nm3

SASDTH4 – Tissue Transglutaminase + 1% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 4.0 nm
Dmax 18.5 nm
VolumePorod 139 nm3

SASDYH4 – Poly-cytosine rC30 RNA in 100 mM NaCl

30mer of poly-cytidine RNA experimental SAS data
30mer of poly-cytidine RNA Kratky plot
Sample: 30mer of poly-cytidine RNA monomer, 9 kDa RNA
Buffer: 100 mM NaCl, 1 mM MOPS, 20 µM EDTA, pH: 7.1
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Dec 7
The Distinct Structural Propensities of Poly-C, A, and U Single-Stranded RNA Biomacromolecules (2026)
...Pollack L
RgGuinier 2.6 nm

SASDCJ4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 400mM KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 400mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.4 nm
Dmax 8.0 nm

SASDRJ4 – GAC rRNA + L11 Protein: SEC-SAXS final

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.6 nm
Dmax 13.0 nm
VolumePorod 48 nm3

SASDTJ4 – Tissue Transglutaminase + 2% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 3.9 nm
Dmax 18.5 nm
VolumePorod 133 nm3

SASDCK4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.25mM MgCl2

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 20mM KCl 0.25mM MgCl2 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.4 nm
Dmax 7.6 nm

SASDRK4 – Diffusive GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDTK4 – Tissue Transglutaminase + 5% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 3.9 nm
Dmax 18.5 nm
VolumePorod 133 nm3

SASDCL4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.50mM MgCl2

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 0.5mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.3 nm
Dmax 7.2 nm

SASDRL4 – Diffusive GAC rRNA + Mg: Time-Resolved 30 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDTL4 – Tissue Transglutaminase + 10% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 4.1 nm
Dmax 21.5 nm
VolumePorod 148 nm3

SASDCM4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 1mM MgCl2

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 1mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L
RgGuinier 2.2 nm
Dmax 7.2 nm

SASDRM4 – Diffusive GAC rRNA + Mg: Time-Resolved 100 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.2 nm
Dmax 9.0 nm
VolumePorod 29 nm3

SASDTM4 – Tissue Transglutaminase R580K mutant

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 6.1 nm
Dmax 32.0 nm
VolumePorod 488 nm3

SASDRN4 – Diffusive GAC rRNA + Mg: Time-Resolved 300 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDTN4 – Tissue Transglutaminase R580K mutant + 100 µM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 5.9 nm
Dmax 27.0 nm
VolumePorod 381 nm3

SASDRP4 – Diffusive GAC rRNA + Mg: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.4 nm
Dmax 13.0 nm
VolumePorod 30 nm3

SASDTP4 – Tissue Transglutaminase R580K mutant + 1 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 5.7 nm
Dmax 30.0 nm
VolumePorod 283 nm3

SASDRQ4 – Diffusive GAC rRNA + Mg: Equilibrium initial

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 34 nm3

SASDTQ4 – Tissue Transglutaminase R580K mutant + 2 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 5.3 nm
Dmax 25.0 nm
VolumePorod 264 nm3

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

50S ribosomal protein L11 experimental SAS data
50S ribosomal protein L11 Kratky plot
Sample: 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L
RgGuinier 2.2 nm
Dmax 11.0 nm
VolumePorod 27 nm3

SASDTR4 – Tissue Transglutaminase R580K mutant + 5 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 243 nm3

SASDSJ5 – 15mer poly-ADP ribose (PAR15) in 100mM NaCl

15mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct
Buffer: 100 mM NaCl, 20 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
...Pollack L, Leung AKL
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDSK5 – 15mer poly-ADP ribose (PAR15) in 100mM NaCl + 1mM MgCl2

15mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct
Buffer: 20 mM Tris-HCl, 1 mM MgCl2, 100 mM NaCl, pH: 7.4
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
...Pollack L, Leung AKL
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 14 nm3

SASDSL5 – 22mer poly-ADP ribose (PAR22) in 100mM NaCl

22mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 22mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 12 kDa synthetic construct
Buffer: 100 mM NaCl, 20 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
...Pollack L, Leung AKL
RgGuinier 3.3 nm
Dmax 14.0 nm
VolumePorod 32 nm3

SASDSM5 – 22mer poly-ADP ribose (PAR22) in 100mM NaCl + 1mM MgCl2

22mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 22mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 12 kDa synthetic construct
Buffer: 20 mM Tris-HCl, 1 mM MgCl2, 100 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
...Pollack L, Leung AKL
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 21 nm3

SASDSN5 – A and C rich mixed sequence single stranded RNA in 100mM NaCl

Mixed sequence single stranded RNA, rich in A and C bases experimental SAS data
Mixed sequence single stranded RNA, rich in A and C bases Kratky plot
Sample: Mixed sequence single stranded RNA, rich in A and C bases monomer, 10 kDa synthetic construct RNA
Buffer: 100 mM NaCl, 10 mM MOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Aug 19
Sequence-dependent conformational preferences of disordered single-stranded RNA. Cell Rep Phys Sci 5(11) (2024)
...Pollack L, Kirmizialtin S
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 15 nm3

SASDKP5 – RNA Short Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDKQ5 – RNA Short Tetraloop Hairpin Duplex in 200 mM KCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.0 nm
Dmax 6.8 nm

SASDKR5 – RNA Short Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.1 nm
Dmax 7.8 nm

SASDKS5 – RNA Short Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDKT5 – RNA Short Tetraloop Hairpin Triplex in 200 mM KCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 1.9 nm
Dmax 6.6 nm

SASDKU5 – RNA Short Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm

SASDKV5 – RNA Long Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.7 nm
Dmax 9.8 nm

SASDKW5 – RNA Long Tetraloop Hairpin Duplex in 200 mM KCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.9 nm
Dmax 10.0 nm

SASDKX5 – RNA Long Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.9 nm
Dmax 10.3 nm

SASDKY5 – RNA Long Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.7 nm
Dmax 10.3 nm

SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDK26 – RNA Long Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
...Pollack L
RgGuinier 2.9 nm
Dmax 18.2 nm

SASDJ96 – Trinucleosomes from Xenopus laevis (African clawed frog)

Histone H3Histone H4Histone H2A type 1Histone H2BNon-linker Ended Trinucleosome DNA experimental SAS data
Histone H3 Histone H4 Histone H2A type 1 Histone H2B Non-linker Ended Trinucleosome DNA Kratky plot
Sample: Histone H3 monomer, 15 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein
Histone H2B monomer, 14 kDa Xenopus laevis protein
Non-linker Ended Trinucleosome DNA monomer, 172 kDa DNA
Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 1 mM DTT 50% w/v sucrose, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Mar 11
Solution structure(s) of trinucleosomes from contrast variation SAXS Nucleic Acids Research (2021)
...Pollack L
RgGuinier 12.9 nm
Dmax 41.8 nm

SASDBD6 – Single stranded poly-deoxythymidine DNA (30mer, dT30)

Poly-deoxythymidine (30mer) experimental SAS data
Single stranded poly-deoxythymidine DNA (30mer, dT30) Rg histogram
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
...Pollack L
RgGuinier 3.0 nm
Dmax 10.7 nm

SASDBE6 – Single stranded poly-deoxyadenosine DNA (30mer, dA30)

Poly-deoxyadenosine (30mer) experimental SAS data
Single stranded poly-deoxyadenosine DNA (30mer, dA30) Rg histogram
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
...Pollack L
RgGuinier 2.7 nm
Dmax 9.5 nm

SASDCT6 – 12N12 nucleosome in 60% sucrose with ADP-BeF3

169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)Histone H2A type 1Histone H2B 1.1Histone H3.2Histone H4 experimental SAS data
12N12 nucleosome in 60% sucrose with ADP-BeF3 Rg histogram
Sample: 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA
Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein
Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein
Histone H3.2 monomer, 15 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH: 7.8
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
...Pollack L, Bowman GD
RgGuinier 4.8 nm
Dmax 14.0 nm

SASDRT6 – Wild type frameshifting pseudoknot from SARS CoV2 virus

Frameshifting pseudoknot from SARS CoV2, wild type experimental SAS data
Frameshifting pseudoknot from SARS CoV2, wild type Kratky plot
Sample: Frameshifting pseudoknot from SARS CoV2, wild type monomer, 23 kDa RNA
Buffer: 50 mM MOPS, 130 mM KCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 May 15
Atomistic structure of the SARS-CoV-2 pseudoknot in solution from SAXS-driven molecular dynamics. Nucleic Acids Res (2023)
...Pollack L
RgGuinier 2.7 nm
Dmax 10.5 nm
VolumePorod 40 nm3

SASDCU6 – Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo)

Chromodomain-helicase-DNA-binding protein 1169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)Histone H2A type 1Histone H2B 1.1Histone H3.2Histone H4 experimental SAS data
Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo) Rg histogram
Sample: Chromodomain-helicase-DNA-binding protein 1 dimer, 266 kDa Saccharomyces cerevisiae protein
169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA
Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein
Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein
Histone H3.2 monomer, 15 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, pH: 7.8
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
...Pollack L, Bowman GD
RgGuinier 5.2 nm
Dmax 12.8 nm

SASDRU6 – Non frameshifting variant of SARS CoV2 frameshifting pseudoknot

Variant: non frameshifting pseudoknot from SARS CoV2 genome experimental SAS data
Variant: non frameshifting pseudoknot from SARS CoV2 genome Kratky plot
Sample: Variant: non frameshifting pseudoknot from SARS CoV2 genome monomer, 23 kDa RNA
Buffer: 50 mM MOPS, 130 mM KCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Jun 12
Atomistic structure of the SARS-CoV-2 pseudoknot in solution from SAXS-driven molecular dynamics. Nucleic Acids Res (2023)
...Pollack L
RgGuinier 2.8 nm
Dmax 13.5 nm
VolumePorod 38 nm3

SASDCV6 – Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with ADP-BeF3

Chromodomain-helicase-DNA-binding protein 1169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)Histone H2A type 1Histone H2B 1.1Histone H3.2Histone H4 experimental SAS data
Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with ADP-BeF3 Rg histogram
Sample: Chromodomain-helicase-DNA-binding protein 1 dimer, 266 kDa Saccharomyces cerevisiae protein
169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA
Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein
Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein
Histone H3.2 monomer, 15 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH: 7.8
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
...Pollack L, Bowman GD
RgGuinier 5.3 nm
Dmax 16.5 nm

SASDCW6 – Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with AMP-PNP

Chromodomain-helicase-DNA-binding protein 1169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)Histone H2A type 1Histone H2B 1.1Histone H3.2Histone H4 experimental SAS data
Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with AMP-PNP Rg histogram
Sample: Chromodomain-helicase-DNA-binding protein 1 dimer, 266 kDa Saccharomyces cerevisiae protein
169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA
Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein
Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein
Histone H3.2 monomer, 15 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, 0.5 mM AMP-PNP, pH: 7.8
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
...Pollack L, Bowman GD
RgGuinier 5.6 nm
Dmax 16.7 nm

SASDBS7 – Nucleosome Core Particle: 1.2 M NaCl, 50% sucrose, 50ms - 10s time resolved data

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B HeterodimerNucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 HeterodimerNucleasome Core Particle with Widom 601 DNA - dsDNA experimental SAS data
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Kratky plot
Sample: Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer dimer, 48 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer dimer, 46 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - dsDNA monomer, 92 kDa Xenopus laevis DNA
Buffer: 20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.2 M NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Apr 14
Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proc Natl Acad Sci U S A 114(2):334-339 (2017)
...Pollack L
RgGuinier 5.8 nm

SASDBT7 – Nucleosome Core Particle: 1.9 M NaCl, 50% sucrose, 50ms - 10s time resolved data

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B HeterodimerNucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 HeterodimerNucleasome Core Particle with Widom 601 DNA - dsDNA experimental SAS data
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Kratky plot
Sample: Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer dimer, 48 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer dimer, 46 kDa Xenopus laevis protein
Nucleasome Core Particle with Widom 601 DNA - dsDNA monomer, 92 kDa Xenopus laevis DNA
Buffer: 20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.9 M NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Apr 14
Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proc Natl Acad Sci U S A 114(2):334-339 (2017)
...Pollack L
RgGuinier 6.8 nm

SASDJV7 – pri-miR16-1 primary microRNA

primary microRNA pri-miR16-1 experimental SAS data
DAMMIF model
Sample: primary microRNA pri-miR16-1 monomer, 36 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
...Pollack L, Showalter S
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 74 nm3

SASDJW7 – pri-miR16-1 primary microRNA in complex with DGCR8-core protein

primary microRNA pri-miR16-1 complexed with DGCR8-core proteinMicroprocessor complex subunit DGCR8 experimental SAS data
DAMMIF model
Sample: primary microRNA pri-miR16-1 complexed with DGCR8-core protein monomer, 36 kDa Homo sapiens RNA
Microprocessor complex subunit DGCR8 monomer, 26 kDa Homo sapiens protein
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, 50% sucrose, DGCR8-core, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
...Pollack L, Showalter S
RgGuinier 5.0 nm
Dmax 17.2 nm
VolumePorod 125 nm3

SASDFA9 – Poly-adenosine rA30 in 20 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 17 nm3

SASDFB9 – Poly-adenosine rA30 in 100 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.4 nm
Dmax 10.7 nm
VolumePorod 14 nm3

SASDFC9 – Poly-adenosine rA30 in 200 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.5 nm
Dmax 10.5 nm
VolumePorod 14 nm3

SASDFD9 – Poly-adenosine rA30 in 400 mM NaCl

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 13 nm3

SASDFE9 – Poly-adenosine rA30 in 600 mM NaCl

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm
VolumePorod 13 nm3

SASDFF9 – Poly-adenosine rA30 in 1 mM MgCl2

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Jul 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.5 nm
Dmax 10.4 nm
VolumePorod 12 nm3

SASDFG9 – Poly-adenosine rA30 in 2 mM MgCl2

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 12 nm3

SASDFH9 – Poly-adenosine rA30 in 5 mM MgCl2

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFJ9 – Poly-uridine rU30 in 20 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDYJ9 – SWAXS from AT DNA duplex (25 base pair)

AT-sequence 25 base-paired DNA experimental SAS data
AT-sequence 25 base-paired DNA Kratky plot
Sample: AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 10 mM MOPS, 150 mM NaCl, 50 µM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jul 16
Interpreting wide-angle X-ray scattering data using DNA duplexes as model systems. IUCrJ 13(Pt 5):650-658 (2026)
...Pollack L
RgGuinier 2.2 nm
Dmax 8.5 nm

SASDFK9 – Poly-uridine rU30 in 100 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.7 nm
Dmax 12.7 nm
VolumePorod 22 nm3

SASDYK9 – SWAXS from Mix DNA duplex (25 base pair)

Mix-sequence 25 base-paired DNA experimental SAS data
Mix-sequence 25 base-paired DNA Kratky plot
Sample: Mix-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 10 mM MOPS, 100 mM KCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2025 Jun 24
Interpreting wide-angle X-ray scattering data using DNA duplexes as model systems. IUCrJ 13(Pt 5):650-658 (2026)
...Pollack L
RgGuinier 2.3 nm
Dmax 8.7 nm

SASDFL9 – Poly-uridine rU30 in 200 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.5 nm
Dmax 11.0 nm
VolumePorod 15 nm3

SASDFM9 – Poly-uridine rU30 in 400 mM NaCl

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.3 nm
Dmax 10.5 nm
VolumePorod 14 nm3

SASDFN9 – Poly-uridine rU30 in 600 mM NaCl

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 14 nm3

SASDFP9 – Poly-uridine rU30 in 1 mM MgCl2

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 17 nm3

SASDFQ9 – Poly-uridine rU30 in 2 mM MgCl2

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 16 nm3

SASDFR9 – Poly-uridine rU30 in 5 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFS9 – Poly-uridine rU30 in 10 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 10 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
...Pollack L
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 14 nm3

SASDCT9 – Bacteriophage phi-X174 - Empty Capsid

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
...Pollack L, Fane BA, Rossmann MG
RgGuinier 14.0 nm

SASDCU9 – Bacteriophage phi-X174 - Before Reaction with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
...Pollack L, Fane BA, Rossmann MG
RgGuinier 12.1 nm

SASDCV9 – Bacteriophage phi-X174 - ~45s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
...Pollack L, Fane BA, Rossmann MG
RgGuinier 12.7 nm

SASDCW9 – Bacteriophage phi-X174 - ~245s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
...Pollack L, Fane BA, Rossmann MG
RgGuinier 12.9 nm

SASDCX9 – Bacteriophage phi-X174 - ~545s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
...Pollack L, Fane BA, Rossmann MG
RgGuinier 12.9 nm

SASDB39 – Single stranded poly-deoxythymidine DNA (30mer, dT30) in 100 mM NaCl

Poly-deoxythymidine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 100mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
...Pollack L
RgGuinier 2.8 nm

SASDB29 – Single stranded poly-deoxyadenosine DNA (30mer, dA30) in 200 mM NaCl

Poly-deoxyadenosine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 200mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
...Pollack L
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 15 nm3

SASDB49 – Single stranded poly-deoxythymidine DNA (30mer, dT30) in 200 mM NaCl

Poly-deoxythymidine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 200mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
...Pollack L
RgGuinier 2.7 nm

SASDBZ8 – Single stranded poly-deoxyadenosine DNA (30mer, dA30) in 100 mM NaCl

Poly-deoxyadenosine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 100mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
...Pollack L
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 16 nm3