Small Angle Scattering Biological Data Bank SASBDB


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22 hits found for Xiao

SASDK92 – Interferon-activable protein 204 from Mus musculus (Mouse) amino-acids 215-619

Interferon-activable protein 204 experimental SAS data

Sample:	Interferon-activable protein 204 monomer, 47 kDa Mus musculus protein
Buffer:	20 mM HEPES, 100 mM KCl, pH: 7.4
Experiment:	SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Mar 14

Structural mechanism of DNA recognition by the p204 HIN domain. Nucleic Acids Res (2021)
...Xiao TS, Jin T

R_g^Guinier	3.1	nm
D_max	9.5	nm
Volume^Porod	28	nm³

SASDEE2 – The N-terminal domain of estrogen receptor alpha

Sample:	Estrogen receptor monomer, 20 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2017 Jun 12

A Metastable Contact and Structural Disorder in the Estrogen Receptor Transactivation Domain. Structure 27(2):229-240.e4 (2019)
...Xiao X, Du Z, Hsieh A, Ko S, Chen Y, Agrawal P, Zheng W, Shi W, Jiang W, Yang L, Chance MR, Surewicz WK, Buck M, Yang S

R_g^Guinier	3.0	nm
D_max	10.0	nm

SASDBR3 – Wild type RNF8 complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin

E3 ubiquitin-protein ligase RNF8Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-C experimental SAS data

Sample:	E3 ubiquitin-protein ligase RNF8 dimer, 35 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8

RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...Xiao AT, Tainer JA, Hendzel MJ, Glover JN

R_g^Guinier	4.5	nm
D_max	18.9	nm
Volume^Porod	119	nm³

SASDBS3 – Wild type RNF8 complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin

E3 ubiquitin-protein ligase RNF8Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CUbiquitin-conjugating enzyme E2 variant 2 experimental SAS data

Sample:	E3 ubiquitin-protein ligase RNF8 dimer, 35 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 variant 2 dimer, 34 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8

R_g^Guinier	5.4	nm
D_max	23.8	nm
Volume^Porod	214	nm³

SASDBT3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CE3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data

Sample:	Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8

R_g^Guinier	4.3	nm
D_max	18.9	nm
Volume^Porod	111	nm³

SASDBU3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CUbiquitin-conjugating enzyme E2 variant 2E3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data

Sample:	Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 variant 2 dimer, 34 kDa Homo sapiens protein E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8

R_g^Guinier	5.2	nm
D_max	23.8	nm
Volume^Porod	192	nm³

SASDKX6 – PSK, an antimicrobial peptide from Chrysomya megacephala

PSK, an antimicrobial peptide from Chrysomya megacephala experimental SAS data

Sample:	PSK, an antimicrobial peptide from Chrysomya megacephala monomer, 10 kDa Chrysomya megacephala protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 7

Crystal and solution structures of a novel antimicrobial peptide from Chrysomya megacephala. Acta Crystallogr D Struct Biol 77(Pt 7):894-903 (2021)
Xiao C, Xiao Z, Hu C, Lu J, Cui L, Zhang Y, Dai Y, Zhang Q, Wang S, Liu W

R_g^Guinier	1.6	nm
D_max	5.0	nm
Volume^Porod	18	nm³

SASDBZ6 – Draxin

Sample:	Draxin monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19

Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
...Xiao J, Zhang Y, Wang JH, Meijers R

R_g^Guinier	4.2	nm
D_max	15.0	nm
Volume^Porod	87	nm³

SASDMJ9 – Plasmid pET11d- 229E710 encoding C-terminally His6-tagged HCoV-229E Nsp7-10 polyprotein

Replicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2

Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R

R_g^Guinier

2.1

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2

R_g^Guinier

3.0