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33 hits found for Ribonuclease

SASDH32 – ...Ribonuclease-A (RNase)

Protein disulfide-isomeraseRibonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique In Silico Pharmacology 12(1) (2024)
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S
RgGuinier 3.0 nm
Dmax 9.1 nm
VolumePorod 85 nm3

SASDE52 – Ribonuclease E from Escherichia coli

Endoribonuclease E experimental SAS data
DAMFILT model
Sample: ...ribonuclease E tetramer, 247 kDa Escherichia coli protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
...Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.0 nm
Dmax 16.1 nm
VolumePorod 468 nm3

SASDH52 – Ribonuclease A (Type III-A) in a solution environment at pH 7.5

Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique In Silico Pharmacology 12(1) (2024)
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 20 nm3

SASDE62 – Ribonuclease E from Yersinia pestis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: ...ribonuclease E tetramer, 248 kDa Yersinia pestis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
...Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 16.4 nm
VolumePorod 470 nm3

SASDE72 – Ribonuclease E from Francisella tularensis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: ...ribonuclease E tetramer, 256 kDa Francisella tularensis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
...Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 17.2 nm
VolumePorod 491 nm3

SASDE82 – Ribonuclease E from Burkholderia pseudomallei

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: ...ribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
...Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 4.8 nm
Dmax 14.9 nm
VolumePorod 437 nm3

SASDE92 – Ribonuclease E from Acinetobacter baumannii

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: ...ribonuclease E tetramer, 254 kDa Acinetobacter baumannii protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
...Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.2 nm
Dmax 18.3 nm
VolumePorod 508 nm3

SASDYH2 – RnaseA at pH 7.4

Bovine Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: ...Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 9
RnaseA
Anthony Giannetti
RgGuinier 1.6 nm
Dmax 60.0 nm
VolumePorod 16 nm3

SASDAN2 – ...ribonuclease A in PBS (WAXS)

Ribonuclease pancreatic experimental SAS data
GASBOR model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 15 nm3

SASDAR2 – ...ribonuclease A in PBS

Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 17 nm3

SASDDL3 – ...ribonuclease A (RNAse)

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: phosphate buffered saline (PBS), pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 1.6 nm
Dmax 5.6 nm
VolumePorod 16 nm3

SASDDM3 – ...ribonuclease A (unfolded RNAse) - with and without urea

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: 10 mM HCl, pH: 1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.3 nm
Dmax 9.0 nm

SASDUA4 – Ribonuclease A Updated Consensus SAXS Data

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 1.5 nm
Dmax 5.0 nm

SASDPP4 – ...Ribonuclease A

Ribonuclease pancreatic experimental SAS data
DAMMIN model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.5 nm
Dmax 4.9 nm
VolumePorod 18 nm3

SASDPU4 – ...Ribonuclease A in 100% v/v D2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.4 nm
Dmax 4.4 nm

SASDEY4 – Ribonuclease A monomer from in-house SEC-SAXS

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 10 nm3

SASDSZ4 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) unliganded form

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot
Sample: ...ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.3 nm
Dmax 9.9 nm
VolumePorod 153 nm3

SASDP25 – ...Ribonuclease A in H2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.5 nm
Dmax 4.1 nm

SASDS25 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Nevirapine

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot
Sample: ...ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.5 nm
Dmax 10.9 nm
VolumePorod 161 nm3

SASDS35 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) unliganded form

Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot
Sample: ...ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.1 nm
Dmax 10.7 nm
VolumePorod 98 nm3

SASDS45 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Efavirenz

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot
Sample: ...ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.7 nm
Dmax 11.3 nm
VolumePorod 178 nm3

SASDS55 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Nevirapine

Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot
Sample: ...ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.5 nm
Dmax 11.3 nm
VolumePorod 134 nm3

SASDS65 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Efavirenz

Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot
Sample: ...ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 4.6 nm
Dmax 15.0 nm
VolumePorod 270 nm3

SASDX27 – Rv2514c VapC52

Ribonuclease experimental SAS data
GASBOR model
Sample: Ribonuclease dimer, 38 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 5
Structural and functional characterization of VapBC52 toxin-antitoxin system from Mycobacterium tuberculosis. Nucleic Acids Res 54(11) (2026)
Singh M, Singh C, Nair AV, Ahmad I, Sharma A, Bhasin M, Jain V, Singh R, Thakur KG
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 50 nm3

SASDX37 – Rv2514c-Rv2515c VapBC52 complex

Ribonuclease VapC52Antitoxin VapB52 experimental SAS data
GASBOR model
Sample: Ribonuclease VapC52 dimer, 37 kDa Mycobacterium tuberculosis protein
Antitoxin VapB52 monomer, 43 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 5
Structural and functional characterization of VapBC52 toxin-antitoxin system from Mycobacterium tuberculosis. Nucleic Acids Res 54(11) (2026)
Singh M, Singh C, Nair AV, Ahmad I, Sharma A, Bhasin M, Jain V, Singh R, Thakur KG
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 107 nm3

SASDPF7 – Ribonuclease A SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 16 nm3

SASDPG7 – Ribonuclease A SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 16 nm3

SASDHW7 – ...ribonuclease toxin LS : Antitoxin RnlB)

mRNA endoribonuclease toxin LSAntitoxin RnlB (C-terminal His-tagged) experimental SAS data
mRNA endoribonuclease toxin LS Antitoxin RnlB (C-terminal His-tagged) Kratky plot
Sample: ...ribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
Antitoxin RnlB (C-terminal His-tagged) monomer, 15 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
...ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 3.5 nm
Dmax 10.0 nm
VolumePorod 161 nm3

SASDHX7 – ...ribonuclease toxin LS)

mRNA endoribonuclease toxin LS experimental SAS data
mRNA endoribonuclease toxin LS Kratky plot
Sample: ...ribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
...ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 3.7 nm
Dmax 10.7 nm
VolumePorod 134 nm3

SASDKL2 – ...ribonuclease toxin LS from Escherichia coli (strain K12)

NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data
MULTIFOXS model
Sample: ...ribonuclease dimer, 62 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jun 27
...ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 88 nm3

SASDHY7 – ...ribonuclease toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged experimental SAS data
mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot
Sample: ...ribonuclease toxin LS - R255A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
...ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 3.7 nm
Dmax 20.1 nm
VolumePorod 133 nm3

SASDKS9 – ...ribonuclease toxin LS (D245R mutant); batch-SAXS measurements in low salt buffer

mRNA endoribonuclease toxin LS (D245R mutant) experimental SAS data
MULTIFOXS model
Sample: ...ribonuclease toxin LS (D245R mutant) dimer, 84 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
...ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 4.3 nm
Dmax 15.9 nm
VolumePorod 183 nm3

SASDHZ7 – ...ribonuclease toxin LS) - R318A single alanine mutant

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged experimental SAS data
mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged Kratky plot
Sample: ...ribonuclease toxin LS - R318A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
...ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 3.4 nm
Dmax 12.7 nm
VolumePorod 124 nm3