Small Angle Scattering Biological Data Bank SASBDB


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38 hits found for Rodriguez

SASDU52 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, apo form

Sample:	Cereblon-midi monomer, 37 kDa protein
Buffer:	20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Mar 1

Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A

R_g^Guinier	2.7	nm
D_max	8.6	nm
Volume^Porod	61	nm³

SASDU62 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Mezigdomide

Cereblon-midimezigdomide experimental SAS data

Sample:	Cereblon-midi monomer, 37 kDa protein mezigdomide monomer, 1 kDa synthetic construct
Buffer:	20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Mar 1

R_g^Guinier	2.3	nm
D_max	7.3	nm
Volume^Porod	60	nm³

SASDU72 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Pomalidomide

Cereblon-midipomalidomide experimental SAS data

Sample:	Cereblon-midi monomer, 37 kDa protein pomalidomide monomer, 0 kDa synthetic construct
Buffer:	20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Mar 1

R_g^Guinier	2.3	nm
D_max	6.9	nm
Volume^Porod	63	nm³

SASDU82 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Iberdomide

Cereblon-midiIberdomide experimental SAS data

Sample:	Cereblon-midi monomer, 37 kDa protein Iberdomide monomer, 0 kDa synthetic construct
Buffer:	20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Mar 1

R_g^Guinier	2.4	nm
D_max	7.7	nm
Volume^Porod	68	nm³

SASDU92 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Lenalidomide

Cereblon-midiLenalidomide experimental SAS data

Sample:	Cereblon-midi monomer, 37 kDa protein Lenalidomide monomer, 0 kDa synthetic construct
Buffer:	20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Mar 1

R_g^Guinier	2.2	nm
D_max	6.2	nm
Volume^Porod	59	nm³

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 6

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	140	nm³

SASDJ64 – Calcium bound Calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
...Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.3	nm
Volume^Porod	27	nm³

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDTL5 – Conjugal transfer accessory disulfide isomerase protein TrbB from the F plasmid (GST-fusion)

Protein TrbB (GST-fusion) experimental SAS data

Sample:	Protein TrbB (GST-fusion) dimer, 93 kDa Escherichia coli (strain … protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5% glycerol, 0.05% NP40, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 23

Structural insights into the disulfide isomerase and chaperone activity of TrbB of the F plasmid type IV secretion system Current Research in Structural Biology 8:100156 (2024)
...Rodriguez C, Audette G

R_g^Guinier	4.0	nm
D_max	18.0	nm
Volume^Porod	153	nm³

SASDTN5 – The D2 domain of E3 ubiquitin-protein ligase DTX3L (I101-Q200)

E3 ubiquitin-protein ligase DTX3L experimental SAS data

E3 ubiquitin-protein ligase DTX3L Kratky plot

Sample:	E3 ubiquitin-protein ligase DTX3L tetramer, 46 kDa Homo sapiens protein
Buffer:	30 mM HEPES, 350 mM NaCl, 5% (v/v) glycerol, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Dec 6

Oligomerisation mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor. bioRxiv (2023)
Vela-Rodríguez C, Yang C, Alanen HI, Eki R, Abbas TA, Maksimainen MM, Glumoff T, Duman R, Wagner A, Paschal BM, Lehtiö L

R_g^Guinier	2.9	nm
D_max	112.0	nm
Volume^Porod	91	nm³

SASDJJ6 – Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1)

N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1) Rg histogram

Sample:	N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 20 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 29

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
...Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, ...

R_g^Guinier	3.5	nm
D_max	14.1	nm
Volume^Porod	46	nm³

SASDJK6 – Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1) Rg histogram

Sample:	Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jul 18

R_g^Guinier	3.9	nm
D_max	17.3	nm
Volume^Porod	66	nm³

SASDJL6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (spectrin repeat rod domain, rod-α-actinin-2)

Rod domain of α-actinin-2 experimental SAS data

Sample:	Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5

R_g^Guinier	6.7	nm
D_max	27.2	nm
Volume^Porod	214	nm³

SASDJM6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd)

Half dimer of α-actinin-2 experimental SAS data

Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd) Rg histogram

Sample:	Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	5.3	nm
D_max	22.0	nm
Volume^Porod	172	nm³

SASDJN6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1)

Rod domain of α-actinin-2Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1) Rg histogram

Sample:	Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) dimer, 43 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	7.6	nm
D_max	28.4	nm
Volume^Porod	371	nm³

SASDVN6 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Boc-VcN

Sample:	Cereblon-midi monomer, 37 kDa synthetic construct protein
Buffer:	20 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 Jun 29

R_g^Guinier	2.3	nm
D_max	9.1	nm
Volume^Porod	63	nm³

SASDJP6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)Half dimer of α-actinin-2 experimental SAS data

Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1) Rg histogram

Sample:	Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	5.8	nm
D_max	22.5	nm
Volume^Porod	242	nm³

SASDVP6 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Boc-AcQ

Sample:	Cereblon-midi monomer, 37 kDa synthetic construct protein
Buffer:	20 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 Jun 29

R_g^Guinier	2.4	nm
D_max	9.6	nm
Volume^Porod	62	nm³

SASDMQ6 – The N-terminus of E3 ubiquitin-protein ligase DTX3L (M1-Q200)

Sample:	E3 ubiquitin-protein ligase DTX3L pentamer, 114 kDa Homo sapiens protein
Buffer:	30 mM HEPES, 350 mM NaCl, 10% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Feb 6

Reconstitution of the DTX3L-PARP9 complex reveals determinants for high affinity heterodimerization and multimeric assembly. Biochem J (2022)
Ashok Y, Vela-Rodríguez C, Yang CS, Alanen HI, Liu F, Paschal BM, Lehtiö L

R_g^Guinier	4.7	nm
D_max	17.0	nm
Volume^Porod	200	nm³

SASDKY6 – ParD2 antitoxin from Vibrio cholerae in low salt pH 8

Sample:	Antitoxin ParD dodecamer, 108 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Entropic pressure controls the oligomerization of the Vibrio cholerae ParD2 antitoxin Acta Crystallographica Section D Structural Biology 77(7):904-920 (2021)
...Rodriguez G, Girardin Y, Volkov A, Singh R, Muruganandam G, Van Dyck J, Sobott F, Versées W, Charlier D, Loris R

R_g^Guinier	3.4	nm
D_max	13.9	nm
Volume^Porod	190	nm³

SASDYV3 – Conjugative protein of the F plasmid Type IV Secretion System, TraW.

Sample:	TraW (Δ1-18) monomer, 26 kDa Escherichia coli (strain … protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5% glycerol, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 23

Solution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system Structural Dynamics 13(2) (2026)
Rodriguez C, Audette G

R_g^Guinier	2.6	nm
D_max	9.0	nm

SASDL56 – 14-3-3 zeta truncated at C-terminus

14-3-3 protein zeta/delta experimental SAS data

Sample:	14-3-3 protein zeta/delta dimer, 53 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 13

A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1. J Mol Biol :167174 (2021)
...Rodriguez E, Milroy LG, Soini L, Adamski CJ, Nitschke L, Davis R, Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J

R_g^Guinier	2.8	nm
D_max	7.9	nm

SASDEU7 – Stator protein FlaG soluble domain

Conserved flagellar protein FlaG soluble domain experimental SAS data

Conserved flagellar protein FlaG soluble domain Kratky plot

Sample:	Conserved flagellar protein FlaG soluble domain monomer, 15 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
...Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV

R_g^Guinier	3.7	nm
D_max	18.0	nm
Volume^Porod	133	nm³

SASDHW7 – RnlA-RnlB toxin-antitoxin complex (mRNA endoribonuclease toxin LS : Antitoxin RnlB)

mRNA endoribonuclease toxin LSAntitoxin RnlB (C-terminal His-tagged) experimental SAS data

mRNA endoribonuclease toxin LS Antitoxin RnlB (C-terminal His-tagged) Kratky plot

Sample:	mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein Antitoxin RnlB (C-terminal His-tagged) monomer, 15 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R

R_g^Guinier	3.5	nm
D_max	10.0	nm
Volume^Porod	161	nm³

SASDLT3 – Ataxin-1 AXH-C

Sample:	Ataxin-1 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 13

R_g^Guinier	4.3	nm
D_max	14.6	nm
Volume^Porod	90	nm³

SASDYU3 – N-terminal truncation mutant, of the conjugative protein of the F plasmid Type IV Secretion System, TraW.

Sample:	TraW (Δ1-67) monomer, 20 kDa Escherichia coli (strain … protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5% glycerol, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 17

Solution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system Structural Dynamics 13(2) (2026)
Rodriguez C, Audette G

R_g^Guinier	2.2	nm
D_max	8.0	nm

SASDES7 – Stator protein complex FlaG/FlaF

Conserved flagellar protein FStator protein FlaG soluble domain experimental SAS data

Sample:	Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

R_g^Guinier	3.2	nm
D_max	12.5	nm
Volume^Porod	109	nm³

SASDHX7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS)

mRNA endoribonuclease toxin LS experimental SAS data

mRNA endoribonuclease toxin LS Kratky plot

Sample:	mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	10.7	nm
Volume^Porod	134	nm³

SASDKL2 – NRD-HEPN - mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)

NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data

Sample:	NRD-HEPN truncated variant of RnlA endoribonuclease dimer, 62 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 27

R_g^Guinier	3.0	nm
D_max	10.4	nm
Volume^Porod	88	nm³

SASDL46 – 14-3-3zeta AXH-C complex

14-3-3 protein zeta/deltaAtaxin-1 AXH-C experimental SAS data

Sample:	14-3-3 protein zeta/delta dimer, 53 kDa Homo sapiens protein Ataxin-1 AXH-C dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 13

R_g^Guinier	4.8	nm
D_max	19.8	nm

SASDEV7 – Stator protein complex FlaG/FlaF-I96Y

Stator protein FlaG soluble domainConserved flagellar protein FlaF-I96Y soluble domain experimental SAS data

Sample:	Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein Conserved flagellar protein FlaF-I96Y soluble domain dimer, 33 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

R_g^Guinier	2.7	nm
D_max	8.2	nm
Volume^Porod	90	nm³

SASDET7 – Stator protein complex FlaG-V118K/FlaF

Conserved flagellar protein FStator protein FlaG-V118K soluble domain experimental SAS data

Sample:	Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein Stator protein FlaG-V118K soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer:	25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10

R_g^Guinier	3.2	nm
D_max	12.5	nm
Volume^Porod	108	nm³

SASDHY7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot

Sample:	mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	20.1	nm
Volume^Porod	133	nm³

SASDKS9 – mRNA endoribonuclease toxin LS (D245R mutant); batch-SAXS measurements in low salt buffer

mRNA endoribonuclease toxin LS (D245R mutant) experimental SAS data

Sample:	mRNA endoribonuclease toxin LS (D245R mutant) dimer, 84 kDa Escherichia coli (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	4.3	nm
D_max	15.9	nm
Volume^Porod	183	nm³

SASDHZ7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R318A single alanine mutant

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged Kratky plot

Sample:	mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.4	nm
D_max	12.7	nm
Volume^Porod	124	nm³

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