Small Angle Scattering Biological Data Bank SASBDB


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184 hits found for Jeffries

SASDJK6 – Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1) Rg histogram

Sample:	Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jul 18

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
...Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos...

R_g^Guinier	3.9	nm
D_max	17.3	nm
Volume^Porod	66	nm³

SASDJL6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (spectrin repeat rod domain, rod-α-actinin-2)

Rod domain of α-actinin-2 experimental SAS data

Sample:	Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5

R_g^Guinier	6.7	nm
D_max	27.2	nm
Volume^Porod	214	nm³

SASDJM6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd)

Half dimer of α-actinin-2 experimental SAS data

Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd) Rg histogram

Sample:	Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	5.3	nm
D_max	22.0	nm
Volume^Porod	172	nm³

SASDJN6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1)

Rod domain of α-actinin-2Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1) Rg histogram

Sample:	Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) dimer, 43 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	7.6	nm
D_max	28.4	nm
Volume^Porod	371	nm³

SASDJP6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)Half dimer of α-actinin-2 experimental SAS data

Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1) Rg histogram

Sample:	Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	5.8	nm
D_max	22.5	nm
Volume^Porod	242	nm³

SASDVV6 – Extended single alpha-helix (SAH) region of Drebrin

Sample:	Drebrin monomer, 10 kDa Homo sapiens protein
Buffer:	17 mM NaH2PO4, 3 mM Na2HPO4, 50 mM NaCl, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jul 7

Dynamic Interchange of Local Residue-Residue Interactions in the Largely Extended Single Alpha-Helix in Drebrin Biochemical Journal (2025)
...Jeffries C, Permi P, Gáspári Z

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	18	nm³

SASDRZ6 – D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A

Thrombospondin type-1 domain-containing protein 7A experimental SAS data

ALPHAFOLD PROTEIN STRUCTURE DATABASE model

Sample:	Thrombospondin type-1 domain-containing protein 7A monomer, 17 kDa Homo sapiens protein
Buffer:	25mM Tris, 150 mM NaCl, 5 mM NaNO3, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Aug 23

Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A (2023)
...Jeffries C, Chojnowski G, Kretz O, Huber T, Tomas N, Wilmanns M

R_g^Guinier	2.5	nm
D_max	9.5	nm
Volume^Porod	28	nm³

SASDR27 – Triple mutant (D116A, H118A, Y176A) of the D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A

Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) experimental SAS data

Triple mutant (D116A, H118A, Y176A) of the D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A Rg histogram

Sample:	Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) monomer, 16 kDa Homo sapiens protein
Buffer:	25mM Tris, 150 mM NaCl, 5 mM NaNO3, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Dec 2

Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A (2023)
...Jeffries C, Chojnowski G, Kretz O, Huber T, Tomas N, Wilmanns M

R_g^Guinier	2.7	nm
D_max	11.5	nm
Volume^Porod	19	nm³

SASDA37 – Surface Protein G (SasG) EG5 repeat protein G51-G52

Sample:	Surface protein G monomer, 24 kDa Staphylococcus aureus protein
Buffer:	20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12

Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
...Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J

R_g^Guinier	4.7	nm
D_max	19.0	nm
Volume^Porod	29	nm³

SASDG37 – 1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data

Sample:	Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein Tegument protein UL51 dimer, 30 kDa Human alphaherpesvirus 1 … protein
Buffer:	20 mM tris, 200 mM NaCl, 3% (v/v) glycerol, 0.25 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 16

Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9 (2020)
...Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC

R_g^Guinier	3.0	nm
D_max	11.5	nm
Volume^Porod	116	nm³

SASDA47 – Surface Protein G (SasG) EG5 repeat protein G51-G53

Sample:	Surface protein G monomer, 39 kDa Staphylococcus aureus protein
Buffer:	20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12

R_g^Guinier	7.7	nm
D_max	30.5	nm
Volume^Porod	49	nm³

SASDG47 – 2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1

Sample:	Tegument protein UL7 dimer, 68 kDa Human alphaherpesvirus 1 … protein Tegument protein UL51 tetramer, 102 kDa Human alphaherpesvirus 1 … protein
Buffer:	20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 16

R_g^Guinier	4.6	nm
D_max	19.7	nm
Volume^Porod	340	nm³

SASDA57 – Surface Protein G (SasG) EG5 repeat protein G51-G54

Sample:	Surface protein G monomer, 53 kDa Staphylococcus aureus protein
Buffer:	20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12

R_g^Guinier	9.7	nm
D_max	38.5	nm
Volume^Porod	58	nm³

SASDG57 – 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1

Sample:	Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein Tegument protein UL51 dimer, 51 kDa Human alphaherpesvirus 1 protein
Buffer:	20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 16

R_g^Guinier	4.0	nm
D_max	18.2	nm
Volume^Porod	160	nm³

SASDA67 – Surface Protein G (SasG) EG5 repeat protein G51-G55

Sample:	Surface protein G monomer, 67 kDa Staphylococcus aureus protein
Buffer:	20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12

R_g^Guinier	12.0	nm
D_max	48.0	nm
Volume^Porod	87	nm³

SASDA77 – Surface Protein G (SasG) EG5 repeat protein G51-G56

Sample:	Surface protein G monomer, 81 kDa Staphylococcus aureus protein
Buffer:	20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12

R_g^Guinier	14.1	nm
D_max	57.0	nm
Volume^Porod	89	nm³

SASDA87 – Surface Protein G (SasG) EG5 repeat protein G51-G57

Sample:	Surface protein G monomer, 95 kDa Staphylococcus aureus protein
Buffer:	20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12

R_g^Guinier	15.9	nm
D_max	63.0	nm
Volume^Porod	122	nm³

SASDA97 – PlaB

Sample:	PlaB tetramer, 220 kDa Legionella pneumophila protein
Buffer:	100 mM Tris 100 mM Nacl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 15

Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions. Sci Rep 5:10734 (2015)
...Jeffries CM, Blanchet CE, Ruskule D, Kuhle K, Flieger A, Schäfer B, Tartsch B, Meijers R, Svergun DI

R_g^Guinier	4.0	nm
D_max	10.5	nm
Volume^Porod	270	nm³

SASDNB7 – C-terminal domain of pUL21 with N-terminal His-tag

Tegument protein UL21 (C-terminal domain) experimental SAS data

Sample:	Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
...Jeffries C, Crump C, Graham S

R_g^Guinier	2.2	nm
D_max	8.5	nm
Volume^Porod	49	nm³

SASDNC7 – miniCERTL with N-terminal His-tag

Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data

Sample:	Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

R_g^Guinier	2.7	nm
D_max	9.1	nm
Volume^Porod	71	nm³

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL

Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data

Sample:	Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

R_g^Guinier	3.3	nm
D_max	13.6	nm
Volume^Porod	84	nm³

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6

Tegument protein UL21Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data

Sample:	Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

R_g^Guinier	4.1	nm
D_max	15.8	nm
Volume^Porod	158	nm³

SASDWG7 – D52A mutant of ferric binding protein (FbpA) in apo (no Fe) form in low ionic strength (LIS) buffer (1/10 PBS)

Iron-utilization periplasmic protein experimental SAS data

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	1 mM Na2HPO4.7H2O, 0.18 mM KH2PO4, 13.7 mM NaCl, 0.27 mM KCl, 5%v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20

Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations The Journal of Chemical Physics 158(8) (2023)
...Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z

R_g^Guinier	2.1	nm
D_max	6.3	nm
Volume^Porod	28	nm³

SASDWH7 – Wild type ferric binding protein (FbpA) in holo (with Fe) form in high ionic strength (HIS) buffer (PBS)

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20

R_g^Guinier	2.0	nm
D_max	6.2	nm
Volume^Porod	34	nm³

SASDWJ7 – Wild type ferric binding protein (FbpA) in apo (no Fe) form in high ionic strength (HIS) buffer (PBS)

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 9

R_g^Guinier	2.1	nm
D_max	6.5	nm
Volume^Porod	39	nm³

SASDWK7 – D52A mutant of ferric binding protein (FbpA) in holo (with Fe) form in high ionic strength (HIS) buffer (PBS)

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 9

R_g^Guinier	2.0	nm
D_max	5.9	nm
Volume^Porod	33	nm³

SASDWL7 – D52A mutant of ferric binding protein (FbpA) in apo (no Fe) form in high ionic strength (HIS) buffer (PBS)

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 19

R_g^Guinier	2.1	nm
D_max	6.2	nm
Volume^Porod	40	nm³

SASDWM7 – D52A mutant of ferric binding protein (FbpA) in holo (with Fe) form in low ionic strength (LIS) buffer (1/10 PBS)

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	1 mM Na2HPO4.7H2O, 0.18 mM KH2PO4, 13.7 mM NaCl, 0.27 mM KCl, 5%v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20

R_g^Guinier	2.0	nm
D_max	6.1	nm
Volume^Porod	24	nm³

SASDWN7 – Wild type ferric binding protein (FbpA) in apo (no Fe) form in low ionic strength (LIS) buffer (1/10 PBS)

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	1 mM Na2HPO4.7H2O, 0.18 mM KH2PO4, 13.7 mM NaCl, 0.27 mM KCl, 5%v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20

R_g^Guinier	2.1	nm
D_max	6.3	nm
Volume^Porod	35	nm³

SASDWP7 – Wild type ferric binding protein (FbpA) in holo (with Fe) form in low ionic strength (LIS) buffer (1/10 PBS)

Sample:	Iron-utilization periplasmic protein monomer, 34 kDa Haemophilus influenzae protein
Buffer:	1 mM Na2HPO4.7H2O, 0.18 mM KH2PO4, 13.7 mM NaCl, 0.27 mM KCl, 5%v/v Glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 20

R_g^Guinier	2.0	nm
D_max	6.1	nm
Volume^Porod	29	nm³

SASDJS7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5

Filaggrin-like protein experimental SAS data

Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5 Rg histogram

Sample:	Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer:	50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22

Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
...Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J

R_g^Guinier	3.5	nm
D_max	14.0	nm
Volume^Porod	46	nm³

SASDKS7 – Hydroxypyruvate aldolase (HpcH/HpaI aldolase), wild-type in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase experimental SAS data

Sample:	HpcH/HpaI aldolase hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer:	20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15

Substrate Induced Movement of the Metal Cofactor between Active and Resting State Angewandte Chemie International Edition (2022)
...Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U

R_g^Guinier	3.3	nm
D_max	9.0	nm
Volume^Porod	224	nm³

SASDJT7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5

Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5 Rg histogram

Sample:	Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer:	50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22

R_g^Guinier	3.7	nm
D_max	15.0	nm
Volume^Porod	48	nm³

SASDKT7 – Hydroxypyruvate aldolase (HpcH/HpaI aldolase), S116A mutant in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase (S116A) experimental SAS data

Sample:	HpcH/HpaI aldolase (S116A) hexamer, 165 kDa Sphingomonas wittichii (strain … protein
Buffer:	20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15

R_g^Guinier	3.3	nm
D_max	9.1	nm
Volume^Porod	222	nm³

SASDKU7 – Hydroxypyruvate aldolase (HpcH/HpaI aldolase), S116C mutant in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase (S116C) experimental SAS data

Sample:	HpcH/HpaI aldolase (S116C) hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer:	20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15

R_g^Guinier	3.3	nm
D_max	9.0	nm
Volume^Porod	223	nm³

SASDQE8 – G. gallus TRPV4 Ankyrin Repeat Domain

Transient receptor potential cation channel subfamily V member 4 experimental SAS data

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 28 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
...Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U

R_g^Guinier	2.3	nm
D_max	11.5	nm
Volume^Porod	41	nm³

SASDHF8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data

Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K Rg histogram

Sample:	Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13

Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
...Jeffries C, Petoukhov M, Svergun D, Bernadó P

R_g^Guinier	1.4	nm
D_max	6.0	nm
Volume^Porod	3	nm³

SASDQF8 – G. gallus TRPV4 N-terminal Domain

G. gallus TRPV4 N-terminal Domain Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 43 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.7	nm
D_max	19.0	nm
Volume^Porod	59	nm³

SASDHG8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 2.51 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide Kratky plot

Sample:	Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer:	20 mM Tris 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13

R_g^Guinier	1.5	nm
D_max	6.4	nm
Volume^Porod	3	nm³

SASDQG8 – G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111

G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 42 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	4.0	nm
D_max	19.5	nm
Volume^Porod	78	nm³

SASDHH8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 1.26 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Sample:	Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer:	20 mM Tris 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13

R_g^Guinier	1.5	nm
D_max	6.0	nm
Volume^Porod	3	nm³

SASDQH8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54

G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 37 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.6	nm
D_max	9.5	nm
Volume^Porod	64	nm³

SASDQJ8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97

G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 33 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.5	nm
D_max	11.9	nm
Volume^Porod	41	nm³

SASDQK8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N104

Transient receptor potential cation channel subfamily V member 4 Kratky plot

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 32 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.8	nm
D_max	13.0	nm
Volume^Porod	49	nm³

SASDQL8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N120

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 29 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.7	nm
D_max	12.5	nm
Volume^Porod	43	nm³

SASDQM8 – G. gallus TRPV4 intrinsically disordered N-terminus

G. gallus TRPV4 intrinsically disordered N-terminus Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 15 kDa Gallus gallus protein
Buffer:	20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.2	nm
D_max	14.5	nm
Volume^Porod	59	nm³

SASDQN8 – G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111

G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 14 kDa Gallus gallus protein
Buffer:	20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.3	nm
D_max	14.5	nm
Volume^Porod	37	nm³

SASDFT8 – The retinoic acid receptor (RAR-RXR heterodimer) bound to the DNA response element HoxB13 DR0

Retinoic acid receptor alpha, RARRetinoic acid receptor RXR-alphaDNA response element HoxB13 DR0 experimental SAS data

Sample:	Retinoic acid receptor alpha, RAR monomer, 41 kDa Mus musculus protein Retinoic acid receptor RXR-alpha monomer, 38 kDa Mus musculus protein DNA response element HoxB13 DR0 monomer, 10 kDa DNA
Buffer:	20 mM Tris, pH 8, 150 mM NaCl, 5% v/v glycerol, 1 mM CHAPS, 4 mM MgSO4, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 19

Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR–RXR Nucleic Acids Research (2020)
...Jeffries C, Svergun D, Rochel N

R_g^Guinier	3.8	nm
D_max	14.5	nm
Volume^Porod	132	nm³

SASDFU8 – The retinoic acid receptor (RAR-RXR heterodimer) bound to the DNA response element F11r DR5.

Retinoic acid receptor alpha, RARRetinoic acid receptor RXR-alphaDNA response element F11r DR5 experimental SAS data

Sample:	Retinoic acid receptor alpha, RAR monomer, 41 kDa Mus musculus protein Retinoic acid receptor RXR-alpha monomer, 38 kDa Mus musculus protein DNA response element F11r DR5 monomer, 13 kDa DNA
Buffer:	20 mM Tris, pH 8, 150 mM NaCl, 5% v/v glycerol, 1 mM CHAPS, 4 mM MgSO4, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 19

Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR–RXR Nucleic Acids Research (2020)
...Jeffries C, Svergun D, Rochel N

R_g^Guinier	4.0	nm
D_max	13.5	nm
Volume^Porod	130	nm³

SASDKW8 – Full-length pUL21 with C-terminal His-tag

Tegument protein UL21 experimental SAS data

Full-length pUL21 with C-terminal His-tag Rg histogram

Sample:	Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread. PLoS Pathog 17(8):e1009824 (2021)
...Jeffries CM, Crump CM, Graham SC

R_g^Guinier	4.2	nm
D_max	18.1	nm
Volume^Porod	90	nm³

SASDQX8 – Bovine serum albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
...Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wol...

R_g^Guinier

3.1

SASDQY8 – Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 15

R_g^Guinier

2.7

SASDQZ8 – Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

5.8

SASDQ29 – Thyroglobulin - reservoir sample storage with liquid jet delivery measured using XFEL

Sample:	Thyroglobulin monomer, 303 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

7.0

SASDQ39 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) - reservoir sample storage with liquid jet delivery measured using XFEL

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Spike glycoprotein (ACE2 receptor binding domain) Kratky plot

Sample:	Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

3.0

SASDHG9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - apo form

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.5	nm
D_max	8.8	nm
Volume^Porod	77	nm³

SASDHH9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.4	nm
D_max	8.5	nm
Volume^Porod	76	nm³

SASDHJ9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and trehalose

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM trehalose, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.4	nm
D_max	8.3	nm
Volume^Porod	76	nm³

SASDHK9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and UDP-glucose

Sample:	Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer:	50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27

Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U

R_g^Guinier	2.4	nm
D_max	8.5	nm
Volume^Porod	73	nm³

SASDLQ9 – Von Willebrand Factor C6 domain wildtype

von Willebrand factor C6 domain wild type experimental SAS data

von Willebrand factor C6 domain wild type Kratky plot

Sample:	von Willebrand factor C6 domain wild type monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 11

Structure and dynamics of the von Willebrand Factor C6 domain Journal of Structural Biology 214(4):107923 (2022)
...Jeffries C, Schneppenheim R, Wilmanns M, Brehm M, Gräter F, Hennig J

R_g^Guinier	1.7	nm
D_max	6.6	nm
Volume^Porod	12	nm³

SASDEX9 – Class II pyruvate aldolase (HpcH/HpaI aldolase), wild-type hexamer in HEPES isolated using SEC-SAXS

Sample:	HpcH/HpaI aldolase hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer:	20 mM HEPES,, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23

EFAMIX , a tool to decompose inline chromatography SAXS data from partially overlapping components Protein Science (2021)
...Jeffries C, Fukuda M, Cheremnykh T, Volkov V, Svergun D

R_g^Guinier	3.3	nm
D_max	9.4	nm
Volume^Porod	233	nm³

SASDPK6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in phosphate conditions

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	1.6	nm
D_max	6.1	nm
Volume^Porod	23	nm³

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data

Sample:	Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Jeffries C

R_g^Guinier	1.8	nm
D_max	5.1	nm
Volume^Porod	37	nm³

SASDEY9 – Collagen VI von Willebrand factor (VWA) double-domain fragment, N5N4

Collagen, type VI, alpha 3 experimental SAS data

Collagen VI von Willebrand factor (VWA) double-domain fragment, N5N4 Rg histogram

Sample:	Collagen, type VI, alpha 3 monomer, 44 kDa Mus musculus protein
Buffer:	20 mM TRIS, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Apr 20

Structure of a collagen VI α3 chain VWA domain array: adaptability and functional implications of myopathy causing mutations Journal of Biological Chemistry :jbc.RA120.014865 (2020)
...Jeffries C, Freiburg C, Meckelburg P, Bird L, Baumann U, Svergun D, Owens R, Werner J, Behrmann E, Paulsson M, Wagener R

R_g^Guinier	2.9	nm
D_max	9.9	nm
Volume^Porod	66	nm³

SASDEZ9 – Collagen VI von Willebrand factor (VWA) four-domain fragment, N6N3

Collagen VI von Willebrand factor (VWA) four-domain fragment, N6N3 Rg histogram

Sample:	Collagen, type VI, alpha 3 monomer, 93 kDa Mus musculus protein
Buffer:	20 mM TRIS, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 4

R_g^Guinier	4.1	nm
D_max	15.6	nm
Volume^Porod	143	nm³

SASDPL6 – 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in phosphate conditions

Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Sample:	Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.2	nm
D_max	8.0	nm
Volume^Porod	24	nm³

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Jeffries C

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	98	nm³

SASDJJ4 – Collagen VI von Willebrand factor (VWA) N2 domain

Collagen alpha-3(VI) chain, N2 domain experimental SAS data

Sample:	Collagen alpha-3(VI) chain, N2 domain monomer, 22 kDa Homo sapiens protein
Buffer:	20 mM TRIS, pH 7.4, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 May 4

R_g^Guinier	1.8	nm
D_max	5.8	nm
Volume^Porod	40	nm³

SASDPM6 – 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions

Stem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Sample:	Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.0	nm
D_max	7.1	nm
Volume^Porod	22	nm³

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Jeffries C

R_g^Guinier	4.0	nm
D_max	13.2	nm
Volume^Porod	211	nm³

SASDPN6 – 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Sample:	Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.8	nm
D_max	10.2	nm
Volume^Porod	33	nm³

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data

Sample:	Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Jeffries C

R_g^Guinier	3.3	nm
D_max	9.3	nm
Volume^Porod	201	nm³

SASDPP6 – 5'-genomic RNA AU extension of SARS-CoV-2 in phosphate conditions

AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Sample:	AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	1.5	nm
D_max	5.4	nm
Volume^Porod	13	nm³

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data

Sample:	Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Jeffries C

R_g^Guinier	5.4	nm
D_max	12.5	nm
Volume^Porod	679	nm³

SASDPQ6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in phosphate conditions

NucleoproteinStem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Nucleoprotein Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.4	nm
D_max	9.0	nm
Volume^Porod	38	nm³

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Jeffries C

R_g^Guinier	2.8	nm
D_max	8.3	nm
Volume^Porod	95	nm³

SASDPR6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

NucleoproteinStem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Nucleoprotein Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	3.4	nm
D_max	13.5	nm
Volume^Porod	51	nm³

SASDPS6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA AU extension of SARS-CoV-2 in phosphate conditions

NucleoproteinAU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Nucleoprotein AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.1	nm
D_max	8.5	nm
Volume^Porod	32	nm³

SASDPT6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) with 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions (mixture)

NucleoproteinStem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data

Nucleoprotein Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.1	nm
D_max	7.8	nm
Volume^Porod	25	nm³

SASDR33 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in HEPES conditions

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer:	25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	1.6	nm
D_max	5.2	nm
Volume^Porod	26	nm³

SASDR43 – 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in HEPES conditions

Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer:	25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.3	nm
D_max	9.5	nm
Volume^Porod	28	nm³

SASDR53 – 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in HEPES conditions

Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer:	25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	2.1	nm
D_max	7.7	nm
Volume^Porod	22	nm³

SASDR63 – 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in HEPES conditions

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer:	25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	3.1	nm
D_max	11.7	nm
Volume^Porod	35	nm³

SASDR73 – 5'-genomic RNA AU extension of SARS-CoV-2 in HEPES conditions

AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot

Sample:	AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer:	25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Jeffries CM, Schlundt A

R_g^Guinier	1.7	nm
D_max	7.0	nm
Volume^Porod	13	nm³

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