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79 hits found for Chaudhuri

SASDEB2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73E

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 19.8 nm
Dmax 23.4 nm

SASDTB2 – WAXS data of poly-histidine tagged Ribose binding protein (16 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.5 nm
Dmax 12.0 nm
VolumePorod 36 nm3

SASDEC2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73A

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 21.8 nm
Dmax 13.6 nm

SASDTC2 – WAXS data of poly-histidine tagged Ribose binding protein (20 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 36 nm3

SASDTD2 – WAXS data of poly-histidine tagged Ribose binding protein (17 mg/mL) from Escherichia coli in the presence of 5 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: Tris 50 mM, 50 mM NaCl, 10% glycerol, 5 mM ribose, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 1.9 nm
Dmax 10.7 nm
VolumePorod 25 nm3

SASDFF2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 245-249)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.5 nm

SASDFG2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 250-254)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 11.4 nm

SASDFH2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 255-259)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.5 nm

SASDFJ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.1 nm

SASDFK2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 265-269)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.9 nm

SASDFL2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.0 nm

SASDFM2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.8 nm

SASDFN2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 280-284)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 9.2 nm

SASDFP2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 285-289)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 8.1 nm

SASDFQ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 290-294)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 8.1 nm

SASDFR2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 15.2 nm

SASDFS2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 280-284)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 13.2 nm

SASDFT2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 285-289)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 15.3 nm

SASDFU2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 290-294)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 14.5 nm

SASDFV2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 295-299)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 12.1 nm

SASDFW2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 300-304)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 13.1 nm

SASDFX2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 11.1 nm

SASDFY2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 310-314)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.6 nm

SASDFZ2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 12.1 nm

SASDF23 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Chaudhuri B
RgGuinier 10.2 nm

SASDHH4 – N-terminal domain of Wag31 from M. tuberculosis

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 tetramer, 34 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 3
Structural basis of self-assembly in the lipid-binding domain of mycobacterial polar growth factor Wag31 IUCrJ 7(4):767-776 (2020)
...Chaudhuri B
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 52 nm3

SASDR37 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin

HOTag-GS-Ubiquitin experimental SAS data
HOTag-GS-Ubiquitin Kratky plot
Sample: HOTag-GS-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 74 nm3

SASDR47 – HOTag6 tetramerization domain followed by a (Gly-Ser)2 linker fused to human monoubiquitin

HOTag6-(GS)2-Ubiquitin experimental SAS data
HOTag6-(GS)2-Ubiquitin Kratky plot
Sample: HOTag6-(GS)2-Ubiquitin tetramer, 52 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 13.7 nm
VolumePorod 69 nm3

SASDR57 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin

HOTag-(GS)4-Ubiquitin experimental SAS data
HOTag-(GS)4-Ubiquitin Kratky plot
Sample: HOTag-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 14.3 nm
VolumePorod 76 nm3

SASDR67 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin

HOTag-(GS)10-Ubiquitin experimental SAS data
HOTag-(GS)10-Ubiquitin Kratky plot
Sample: HOTag-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.0 nm
Dmax 14.8 nm
VolumePorod 84 nm3

SASDR77 – HOTag6 tetramerization domain followed by a (Gly-Ser)25 linker fused to human monoubiquitin

HOTag6-(GS)25-Ubiquitin experimental SAS data
HOTag6-(GS)25-Ubiquitin Kratky plot
Sample: HOTag6-(GS)25-Ubiquitin tetramer, 65 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.7 nm
Dmax 19.3 nm
VolumePorod 117 nm3

SASDR87 – HOTag6 tetramerization domain followed by a (Gly-Ser)50 linker fused to human monoubiquitin

HOTag-(GS)50-Ubiquitin experimental SAS data
HOTag-(GS)50-Ubiquitin Kratky plot
Sample: HOTag-(GS)50-Ubiquitin tetramer, 80 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.6 nm
Dmax 22.7 nm
VolumePorod 193 nm3

SASDR97 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin

HOTag-PA-Ubiquitin experimental SAS data
HOTag-PA-Ubiquitin Kratky plot
Sample: HOTag-PA-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 12.9 nm
VolumePorod 73 nm3

SASDRA7 – HOTag6 tetramerization domain followed by a (Pro-Ala)2 linker fused to human monoubiquitin

HOTag6-(PA)2-Ubiquitin experimental SAS data
HOTag6-(PA)2-Ubiquitin Kratky plot
Sample: HOTag6-(PA)2-Ubiquitin tetramer, 52 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 15.0 nm
VolumePorod 74 nm3

SASDRB7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin

HOTag-(PA)4-Ubiquitin experimental SAS data
HOTag-(PA)4-Ubiquitin Kratky plot
Sample: HOTag-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 78 nm3

SASDRC7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data
HOTag-(PA)25-Ubiquitin Kratky plot
Sample: HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 18.4 nm
VolumePorod 106 nm3

SASDRD7 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)-Ubiquitin experimental SAS data
HOTag6-(GS)-Ubiquitin Kratky plot
Sample: HOTag6-(GS)-Ubiquitin tetramer, 51 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 70 nm3

SASDRE7 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)4-Ubiquitin experimental SAS data
HOTag6-(GS)4-Ubiquitin Kratky plot
Sample: HOTag6-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 12.8 nm
VolumePorod 114 nm3

SASDRF7 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)10-Ubiquitin experimental SAS data
HOTag6-(GS)10-Ubiquitin Kratky plot
Sample: HOTag6-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.2 nm
VolumePorod 86 nm3

SASDRG7 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)-Ubiquitin experimental SAS data
HOTag6-(PA)-Ubiquitin Kratky plot
Sample: HOTag6-(PA)-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 12.4 nm
VolumePorod 71 nm3

SASDRH7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)4-Ubiquitin experimental SAS data
HOTag6-(PA)4-Ubiquitin Kratky plot
Sample: HOTag6-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.7 nm
VolumePorod 80 nm3

SASDRJ7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)10-Ubiquitin experimental SAS data
HOTag6-(PA)10-Ubiquitin Kratky plot
Sample: HOTag6-(PA)10-Ubiquitin tetramer, 57 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 19.0 nm
VolumePorod 105 nm3

SASDRK7 – M1-linked tetraubiquitin, M1(1-72)-Ub4

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 41 nm3

SASDRL7 – M1-linked tetraubiquitin, M1(1-73)-Ub4

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.3 nm
Dmax 12.8 nm
VolumePorod 41 nm3

SASDRM7 – M1-linked tetraubiquitin, M1(1-74)-Ub4

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.2 nm
Dmax 12.2 nm
VolumePorod 43 nm3

SASDRN7 – M1-linked tetraubiquitin Pro-Ser/Gly-Ser fusion, M1(1-76)-PS(GS)4-Ub4

Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 experimental SAS data
Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 Kratky plot
Sample: Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 monomer, 36 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.4 nm
Dmax 13.9 nm
VolumePorod 50 nm3

SASDRP7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)3A-Ub4

Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub experimental SAS data
Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub Kratky plot
Sample: Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub monomer, 39 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.2 nm
Dmax 18.2 nm
VolumePorod 62 nm3

SASDRQ7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)6A-Ub4

Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub experimental SAS data
Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub Kratky plot
Sample: Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub monomer, 43 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.2 nm
Dmax 21.2 nm
VolumePorod 108 nm3

SASDMY7 – Poly-histidine tagged Myosin X component at 8979 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.5 nm
VolumePorod 24 nm3

SASDMZ7 – Poly-histidine tagged Myosin X component at 8981 eV

Unconventional myosin-X experimental SAS data
Unconventional myosin-X Kratky plot
Sample: Unconventional myosin-X dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM28 – Poly-histidine tagged Myosin X component at 8983 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM38 – Poly-histidine tagged Myosin X component at 8985 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM48 – Poly-histidine tagged Myosin X component at 8987 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM58 – Poly-histidine tagged Myosin X component at 8989 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM68 – Poly-histidine tagged Myosin X component at 8991 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDM78 – Poly-histidine tagged Myosin X component at 8993 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.2 nm
Dmax 8.0 nm

SASDM88 – Poly-histidine tagged Myosin X component with CuSO4 at 8979 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 10.5 nm
VolumePorod 27 nm3

SASDM98 – Poly-histidine tagged Myosin X component with CuSO4 at 8981 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.5 nm
Dmax 11.5 nm
VolumePorod 27 nm3

SASDMA8 – Poly-histidine tagged Myosin X component with CuSO4 at 8983 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 10.5 nm
VolumePorod 27 nm3

SASDMB8 – Poly-histidine tagged Myosin X component with CuSO4 at 8985 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.5 nm
Dmax 11.5 nm
VolumePorod 27 nm3

SASDMC8 – Poly-histidine tagged Myosin X component with CuSO4 at 8987 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.0 nm
VolumePorod 28 nm3

SASDMD8 – Poly-histidine tagged Myosin X component with CuSO4 at 8989 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 27 nm3

SASDME8 – Poly-histidine tagged Myosin X component with CuSO4 at 8991 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 28 nm3

SASDMF8 – Poly-histidine tagged Myosin X component with CuSO4 at 8993 eV

Unconventional myosin-X component experimental SAS data
Unconventional myosin-X component Kratky plot
Sample: Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer: HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16
K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
...Chaudhuri B
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 28 nm3

SASDRK8 – HOTag6 tetramerization domain followed by a (Pro-Ala)25 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data
HOTag-(PA)25-Ubiquitin Kratky plot
Sample: HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
...Chaudhuri A, Schmit J, Castañeda C
RgGuinier 6.3 nm
Dmax 24.9 nm
VolumePorod 180 nm3

SASDSB9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.6 nm
VolumePorod 35 nm3

SASDSC9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.4 nm
VolumePorod 37 nm3

SASDSD9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 39 nm3

SASDSE9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.0 nm
Dmax 8.3 nm
VolumePorod 35 nm3

SASDSF9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.0 nm
Dmax 7.6 nm
VolumePorod 36 nm3

SASDSG9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
...Chaudhuri B
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 39 nm3

SASDV63 – B2 SINE ribozyme wildtype

B2 short interspaced nuclear element (SINE) RNA experimental SAS data
DAMMIN model
Sample: B2 short interspaced nuclear element (SINE) RNA monomer, 57 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 20
Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme. Commun Biol (2026)
Singhal A, Mrozowich T, Rivera C, Chaudhury SN, Xu L, Aguilar R, Badmalia M, Lee JT, Patel TR, Sanbonmatsu KY
RgGuinier 5.4 nm
Dmax 17.3 nm
VolumePorod 131 nm3

SASDEU7 – Stator protein FlaG soluble domain

Conserved flagellar protein FlaG soluble domain experimental SAS data
Conserved flagellar protein FlaG soluble domain Kratky plot
Sample: Conserved flagellar protein FlaG soluble domain monomer, 15 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.7 nm
Dmax 18.0 nm
VolumePorod 133 nm3

SASDV93 – B2 SINE ribozyme with B2J Mutation

B2 short interspaced nuclear element (SINE) RNA with two point mutations experimental SAS data
DAMMIN model
Sample: B2 short interspaced nuclear element (SINE) RNA with two point mutations monomer, 57 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 20
Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme. Commun Biol (2026)
Singhal A, Mrozowich T, Rivera C, Chaudhury SN, Xu L, Aguilar R, Badmalia M, Lee JT, Patel TR, Sanbonmatsu KY
RgGuinier 5.5 nm
Dmax 17.3 nm
VolumePorod 136 nm3

SASDES7 – Stator protein complex FlaG/FlaF

Conserved flagellar protein FStator protein FlaG soluble domain experimental SAS data
MULTIFOXS model
Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein
Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 109 nm3

SASDV83 – B2 SINE ribozyme with deletion mutation 96-105

B2 short interspaced nuclear element (SINE) RNA with deletion mutation 96-105 experimental SAS data
DAMMIN model
Sample: B2 short interspaced nuclear element (SINE) RNA with deletion mutation 96-105 monomer, 54 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Dec 18
Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme. Commun Biol (2026)
Singhal A, Mrozowich T, Rivera C, Chaudhury SN, Xu L, Aguilar R, Badmalia M, Lee JT, Patel TR, Sanbonmatsu KY
RgGuinier 5.5 nm
Dmax 17.5 nm
VolumePorod 135 nm3

SASDEV7 – Stator protein complex FlaG/FlaF-I96Y

Stator protein FlaG soluble domainConserved flagellar protein FlaF-I96Y soluble domain experimental SAS data
BILBOMD model
Sample: Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Conserved flagellar protein FlaF-I96Y soluble domain dimer, 33 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 90 nm3

SASDV73 – B2 SINE ribozyme with deletion mutation 81-124

B2 short interspaced nuclear element (SINE) RNA with deletion mutation 81-124 experimental SAS data
DAMMIF model
Sample: B2 short interspaced nuclear element (SINE) RNA with deletion mutation 81-124 dimer, 86 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Aug 19
Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme. Commun Biol (2026)
Singhal A, Mrozowich T, Rivera C, Chaudhury SN, Xu L, Aguilar R, Badmalia M, Lee JT, Patel TR, Sanbonmatsu KY
RgGuinier 8.1 nm
Dmax 22.1 nm

SASDET7 – Stator protein complex FlaG-V118K/FlaF

Conserved flagellar protein FStator protein FlaG-V118K soluble domain experimental SAS data
MULTIFOXS model
Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein
Stator protein FlaG-V118K soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 108 nm3