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318 hits found for waxs

SASDHM5 – Apolipoprotein-D plus progesterone

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.0 nm
VolumePorod 161 nm3

SASDJM5 – Modified nucleolysin TIA-1 isoform p40 (TIA-1 APO)

Modified Nucleolysin TIA-1 isofrom p40 experimental SAS data
Modified Nucleolysin TIA-1 isofrom p40 Kratky plot
Sample: Modified Nucleolysin TIA-1 isofrom p40 monomer, 42 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 60 mM KCl, 0.5 M arginine-HCl, 1 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2020 Jul 7
Tandem RNA binding sites induce self-association of the stress granule marker protein TIA-1. Nucleic Acids Res (2021)
Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA
RgGuinier 3.2 nm
Dmax 12.7 nm
VolumePorod 62 nm3

SASDKM5 – Metastasis-Associated Lung Adenocarcinoma Transcript 1

Metastasis-Associated Lung Adenocarcinoma Transcript 1 experimental SAS data
Metastasis-Associated Lung Adenocarcinoma Transcript 1 Kratky plot
Sample: Metastasis-Associated Lung Adenocarcinoma Transcript 1 monomer, 23 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 μM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
...WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.3 nm
Dmax 7.9 nm

SASDHN5 – Apolipoprotein-D plus sphingomyelin

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 9.9 nm
VolumePorod 171 nm3

SASDKN5 – 30nt Single-stranded RNA

30nt Single-stranded RNA experimental SAS data
30nt Single-stranded RNA Kratky plot
Sample: 30nt Single-stranded RNA monomer, 10 kDa RNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.2 nm
Dmax 8.2 nm

SASDKP5 – RNA Short Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDHQ5 – Ile-Leu-Gln-Ile-Asn-Ser (ILQINS) hexapeptide self-assembly

Ile-Leu-Gln-Ile-Asn-Ser peptide experimental SAS data
OTHER model
Sample: Ile-Leu-Gln-Ile-Asn-Ser peptide , 1 kDa synthetic construct protein
Buffer: pure (MQ, 18 MΩ) Water, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Nov 1
Amyloid Evolution: Antiparallel Replaced by Parallel. Biophys J (2020)
Zanjani AAH, Reynolds NP, Zhang A, Schilling T, Mezzenga R, Berryman JT

SASDKQ5 – RNA Short Tetraloop Hairpin Duplex in 200 mM KCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.0 nm
Dmax 6.8 nm

SASDKR5 – RNA Short Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.1 nm
Dmax 7.8 nm

SASDKS5 – RNA Short Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDKT5 – RNA Short Tetraloop Hairpin Triplex in 200 mM KCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.6 nm

SASDNT5 – Bradyzoite Pseudokinase 1 (amino acids 61-377)

Rhoptry kinase family protein experimental SAS data
Sample: Rhoptry kinase family protein tetramer, 143 kDa Toxoplasma gondii (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 19
Divergent kinase WNG1 is regulated by phosphorylation of an atypical activation sub-domain. Biochem J (2022)
Dewangan PS, Beraki TG, Paiz EA, Appiah Mensah D, Chen Z, Reese ML
RgGuinier 3.8 nm
Dmax 11.5 nm
VolumePorod 207 nm3

SASDKU5 – RNA Short Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm

SASDDV5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum

4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 159 nm3

SASDKV5 – RNA Long Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 9.8 nm

SASDDW5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum + pyruvate

4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, 5mM sodium pyruvate, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.3 nm
Dmax 8.9 nm
VolumePorod 165 nm3

SASDKW5 – RNA Long Tetraloop Hairpin Duplex in 200 mM KCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.0 nm

SASDPW5 – ...WAXS)

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 50 mM NaCl, 50 μM EDTA, 10 mM MOPS, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Sep 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.8 nm
Dmax 13.3 nm
VolumePorod 58 nm3

SASDKX5 – RNA Long Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.3 nm

SASDKY5 – RNA Long Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 10.3 nm

SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDK26 – RNA Long Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 18.2 nm

SASDD66 – Phox homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha experimental SAS data
DAMFILT model
Sample: Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha monomer, 33 kDa Homo sapiens protein
Buffer: 25 mM Tris 200 mM NaCl 5% Glycerol 0.5 mM TCEP, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 20
Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α. Structure (2018)
Chen KE, Tillu VA, Chandra M, Collins BM
RgGuinier 2.6 nm
Dmax 9.3 nm
VolumePorod 43 nm3

SASDC76 – Extracellular domain of human B-lymphocyte cell receptor CD22

CD22 extracellular domain experimental SAS data
DAMMIF model
Sample: CD22 extracellular domain monomer, 90 kDa Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 9
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 21
Molecular basis of human CD22 function and therapeutic targeting. Nat Commun 8(1):764 (2017)
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
RgGuinier 8.0 nm
Dmax 30.6 nm

SASDD76 – Phox Homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α) in complex with inositol-hexaphosphate (IP6)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha experimental SAS data
DAMFILT model
Sample: Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha monomer, 33 kDa Homo sapiens protein
Buffer: 25 mM Tris 200 mM NaCl 5% Glycerol 0.5 mM TCEP 4 mM InsP6, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 20
Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α. Structure (2018)
Chen KE, Tillu VA, Chandra M, Collins BM
RgGuinier 2.6 nm
Dmax 9.3 nm
VolumePorod 48 nm3

SASDC86 – Extracellular domain of human B-lymphocyte cell receptor CD22 in complex with alpha 2,6 sialyllactose

CD22 extracellular domainalpha(2,6)-Sialyllactose experimental SAS data
DAMMIF model
Sample: CD22 extracellular domain monomer, 90 kDa Homo sapiens protein
alpha(2,6)-Sialyllactose , 1 kDa
Buffer: 20 mM Tris 150 mM NaCl, pH: 9
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 21
Molecular basis of human CD22 function and therapeutic targeting. Nat Commun 8(1):764 (2017)
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
RgGuinier 8.1 nm
Dmax 29.8 nm

SASDEA6 – Staphylococcus aureus N-acetylglucosamine-6-phosphate deacetylase dimer

N-acetylglucosamine-6-phosphate deacetylase experimental SAS data
PYMOL model
Sample: N-acetylglucosamine-6-phosphate deacetylase dimer, 86 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris-HCl 150 mM NaCl, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Apr 26
Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus. FEBS Lett (2018)
Davies JS, Coombes D, Horne CR, Pearce FG, Friemann R, North RA, Dobson RCJ
RgGuinier 3.2 nm
Dmax 10.1 nm
VolumePorod 107 nm3

SASDFA6 – Proteolytic fragment of phage flagella binding tail protein YSD1_29 (amino acids 373-1296)

Flagella binding tail protein experimental SAS data
DAMMIF model
Sample: Flagella binding tail protein monomer, 103 kDa Salmonella virus Chi protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 4
The flagellotropic bacteriophage YSD1 targets Salmonella Typhi with a Chi-like protein tail fibre. Mol Microbiol (2019)
Dunstan RA, Pickard D, Dougan S, Goulding D, Cormie C, Hardy J, Li F, Grinter R, Harcourt K, Yu L, Song J, Schreiber F, Choudhary J, Clare S, Coulibaly F, Strugnell RA, Dougan G, Lithgow T
RgGuinier 5.6 nm
Dmax 27.4 nm
VolumePorod 155 nm3

SASDEB6 – Staphylococcus aureus glucosamine-6-phosphate deaminase

Glucosamine-6-phosphate deaminase experimental SAS data
PYMOL model
Sample: Glucosamine-6-phosphate deaminase dimer, 57 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris-HCl 150 mM NaCl, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Apr 26
Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus. FEBS Lett (2018)
Davies JS, Coombes D, Horne CR, Pearce FG, Friemann R, North RA, Dobson RCJ
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 84 nm3

SASDFB6 – The periplasmically localised protease PqqL from Escherichia coli

Zinc protease PqqL experimental SAS data
DAMMIF model
Sample: Zinc protease PqqL monomer, 102 kDa Escherichia coli protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Jun 14
Protease-associated import systems are widespread in Gram-negative bacteria. PLoS Genet 15(10):e1008435 (2019)
Grinter R, Leung PM, Wijeyewickrema LC, Littler D, Beckham S, Pike RN, Walker D, Greening C, Lithgow T
RgGuinier 4.0 nm
Dmax 13.7 nm
VolumePorod 207 nm3

SASDJC6 – Human Vitamin K-dependent protein C

Vitamin K-dependent protein C experimental SAS data
DAMFILT model
Sample: Vitamin K-dependent protein C monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 145 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jul 14
Zymogen and activated protein C have similar structural architecture. J Biol Chem (2020)
Stojanovski BM, Pelc LA, Zuo X, Di Cera E
RgGuinier 3.6 nm
Dmax 14.0 nm

SASDJD6 – Human Vitamin K-dependent Activated Protein C

Vitamin K-dependent protein C experimental SAS data
DAMFILT model
Sample: Vitamin K-dependent protein C monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 145 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Aug 17
Zymogen and activated protein C have similar structural architecture. J Biol Chem (2020)
Stojanovski BM, Pelc LA, Zuo X, Di Cera E
RgGuinier 3.7 nm
Dmax 14.0 nm

SASDDQ6 – The ferredoxin protease, FusC

Ferredoxin Protease experimental SAS data
Ferredoxin Protease Kratky plot
Sample: Ferredoxin Protease monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 6
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 12.8 nm
VolumePorod 152 nm3

SASDDR6 – The ferredoxin protease, FusC, E83A mutant

Ferredoxin protease E83A mutant experimental SAS data
Ferredoxin protease E83A mutant Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 6
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 12.7 nm
VolumePorod 152 nm3

SASDMR6 – 5-10-5 gapmer phosphorothioate antisense oligonucleotide

5-10-5 gapmer phosphorothioate antisense oligonucleotide experimental SAS data
DAMFILT model
Sample: 5-10-5 gapmer phosphorothioate antisense oligonucleotide dimer, 14 kDa
Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Apr 28
Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1. Nucleic Acids Res (2021)
Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS
RgGuinier 1.8 nm
Dmax 8.5 nm
VolumePorod 15 nm3

SASDDS6 – The ferredoxin protease, FusC, with Arabidopsis ferredoxin (co-SEC-SAXS)

Ferredoxin ProteaseArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin Protease Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin Protease monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 6
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.2 nm
VolumePorod 156 nm3

SASDES6 – The 11S subunit of the Plasmodium falciparum proteasome, PA28

Proteasome activator PA28 experimental SAS data
CORAL model
Sample: Proteasome activator PA28 heptamer, 232 kDa Plasmodium falciparum protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Dec 2
The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis. Nat Microbiol 4(11):1990-2000 (2019)
Xie SC, Metcalfe RD, Hanssen E, Yang T, Gillett DL, Leis AP, Morton CJ, Kuiper MJ, Parker MW, Spillman NJ, Wong W, Tsu C, Dick LR, Griffin MDW, Tilley L
RgGuinier 4.3 nm
Dmax 12.9 nm
VolumePorod 484 nm3

SASDMS6 – Non-POU domain-containing octamer-binding protein (NONO homodimer)

Non-POU domain-containing octamer-binding protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Apr 28
Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1. Nucleic Acids Res (2021)
Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 96 nm3

SASDDT6 – The ferredoxin protease, FusC, E83A mutant with Arabidopsis ferredoxin (co-SEC-SAXS)

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 6
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.6 nm
Dmax 12.7 nm
VolumePorod 161 nm3

SASDMT6 – Non-POU domain-containing octamer-binding protein (NONO homodimer) bound to an antisense oligonucleotide

Non-POU domain-containing octamer-binding protein5-10-5 gapmer phosphorothioate antisense oligonucleotide tetramer experimental SAS data
SASREF model
Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein
5-10-5 gapmer phosphorothioate antisense oligonucleotide tetramer tetramer, 28 kDa
Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Apr 28
Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1. Nucleic Acids Res (2021)
Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS
RgGuinier 3.9 nm
Dmax 18.4 nm
VolumePorod 153 nm3

SASDDU6 – The ferredoxin protease, FusC, E83A mutant

Ferredoxin protease E83A mutant experimental SAS data
Ferredoxin protease E83A mutant Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.8 nm
VolumePorod 152 nm3

SASDDV6 – The ferredoxin protease, FusC, E83A mutant + 3 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.8 nm
VolumePorod 150 nm3

SASDBW6 – Suppressor of Copper Sensitivity C protein (ScsC) from P. mirabilis (mutant)

Suppressor of Copper Sensitivity C protein (mutant) experimental SAS data
Suppressor of Copper Sensitivity C protein (ScsC) from P. mirabilis (mutant) Rg histogram
Sample: Suppressor of Copper Sensitivity C protein (mutant) trimer, 73 kDa Proteus mirabilis protein
Buffer: 25 mM HEPES 150mM NaCl, 1mM DTT,, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Jul 22
A shape-shifting redox foldase contributes to Proteus mirabilis copper resistance. Nat Commun 8:16065 (2017)
Furlong EJ, Lo AW, Kurth F, Premkumar L, Totsika M, Achard MES, Halili MA, Heras B, Whitten AE, Choudhury HG, Schembri MA, Martin JL
RgGuinier 4.4 nm
Dmax 13.5 nm
VolumePorod 108 nm3

SASDDW6 – The ferredoxin protease, FusC, E83A mutant + 5 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.7 nm
VolumePorod 148 nm3

SASDDX6 – The ferredoxin protease, FusC, E83A mutant + 10 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.6 nm
VolumePorod 149 nm3

SASDMX6 – 40-mer single stranded DNA inhibitor of DNA dC->dU-editing enzyme APOBEC3G

40-mer single stranded inhibitory DNA experimental SAS data
DAMFILT model
Sample: 40-mer single stranded inhibitory DNA monomer, 12 kDa DNA
Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Aug 8
Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity Viruses 14(9):1974 (2022)
Barzak F, Ryan T, Mohammadzadeh N, Harjes S, Kvach M, Kurup H, Krause K, Chelico L, Filichev V, Harjes E, Jameson G
RgGuinier 3.2 nm
Dmax 11.8 nm
VolumePorod 26 nm3

SASDDY6 – The ferredoxin protease, FusC, E83A mutant + 20 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.6 nm
VolumePorod 155 nm3

SASDMY6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length

DNA dC->dU-editing enzyme APOBEC-3G experimental SAS data
DAMFILT model
Sample: DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein
Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Aug 6
Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity Viruses 14(9):1974 (2022)
Barzak F, Ryan T, Mohammadzadeh N, Harjes S, Kvach M, Kurup H, Krause K, Chelico L, Filichev V, Harjes E, Jameson G
RgGuinier 4.2 nm
Dmax 13.3 nm
VolumePorod 350 nm3

SASDDZ6 – The ferredoxin protease, FusC, E83A mutant + 30 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 156 nm3

SASDMZ6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: tetramer

DNA dC->dU-editing enzyme APOBEC-3G40-mer single stranded inhibitory DNA experimental SAS data
DAMFILT model
Sample: DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein
40-mer single stranded inhibitory DNA dimer, 24 kDa DNA
Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Aug 6
Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity Viruses 14(9):1974 (2022)
Barzak F, Ryan T, Mohammadzadeh N, Harjes S, Kvach M, Kurup H, Krause K, Chelico L, Filichev V, Harjes E, Jameson G
RgGuinier 4.7 nm
Dmax 16.2 nm
VolumePorod 395 nm3

SASDD27 – The ferredoxin protease, FusC, E83A mutant + 50 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 160 nm3

SASDM27 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: monomer

40-mer single stranded inhibitory DNADNA dC->dU-editing enzyme APOBEC-3G experimental SAS data
DAMFILT model
Sample: 40-mer single stranded inhibitory DNA monomer, 12 kDa DNA
DNA dC->dU-editing enzyme APOBEC-3G monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Aug 6
Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity Viruses 14(9):1974 (2022)
Barzak F, Ryan T, Mohammadzadeh N, Harjes S, Kvach M, Kurup H, Krause K, Chelico L, Filichev V, Harjes E, Jameson G
RgGuinier 3.1 nm
Dmax 10.0 nm
VolumePorod 118 nm3

SASDD37 – The ferredoxin protease, FusC, E83A mutant + 70 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 165 nm3

SASDD47 – The ferredoxin protease, FusC, E83A mutant + 100 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.7 nm
VolumePorod 177 nm3

SASDD57 – The ferredoxin protease, FusC, E83A mutant + 150 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.8 nm
Dmax 14.2 nm
VolumePorod 178 nm3

SASDV57 – Full-length SFPQ in high salt buffer

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Oct 22
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 7.9 nm
Dmax 42.5 nm
VolumePorod 485 nm3

SASDD67 – The ferredoxin protease, FusC, E83A mutant + 200 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 175 nm3

SASDV67 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707)

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data
Splicing Factor Proline/Glutamine rich (1-598) Kratky plot
Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2023 Mar 13
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 7.6 nm
Dmax 34.3 nm
VolumePorod 400 nm3

SASDD77 – The ferredoxin protease, FusC, E83A mutant + 300 µM Arabidopsis ferredoxin

Ferredoxin protease E83A mutantArabidopsis ferredoxin 2 experimental SAS data
Ferredoxin protease E83A mutant Arabidopsis ferredoxin 2 Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 180 nm3

SASDV77 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707) in low salt buffer

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data
Splicing Factor Proline/Glutamine rich (1-598) Kratky plot
Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2023 Mar 13
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 6.1 nm
Dmax 28.0 nm
VolumePorod 315 nm3

SASDN97 – Human thryoglobulin measured using SEC-SAXS

Thyroglobulin experimental SAS data
DAMMIN model
Sample: Thyroglobulin dimer, 610 kDa Homo sapiens protein
Buffer: phosphate buffered saline, 150mM NaCl, pH: 8
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Jul 17
...WAXS data
James Byrnes
RgGuinier 7.7 nm
Dmax 26.3 nm
VolumePorod 1800 nm3

SASDW97 – Recombination directionality factor (6H-RdfS) from M.japonicum bound to 40mer DNA

Recombination directionality factor RdfSattP_8 40-mer DNA experimental SAS data
OTHER model
Sample: Recombination directionality factor RdfS tetramer, 52 kDa Mesorhizobium japonicum R7A protein
attP_8 40-mer DNA dimer, 25 kDa DNA
Buffer: 150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Jun 19
Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix–turn–helix protein RdfS Nucleic Acids Research 53(6) (2025)
Verdonk C, Agostino M, Eto K, Hall D, Bond C, Ramsay J
RgGuinier 3.8 nm
Dmax 13.8 nm
VolumePorod 134 nm3

SASDNA7 – Bovine thyroglobulin in phosphate buffered saline/NaCl

Thyroglobulin experimental SAS data
DAMMIF model
Sample: Thyroglobulin dimer, 606 kDa Bos taurus protein
Buffer: phosphate buffered saline, 150mM NaCl, pH: 8
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Jul 17
...WAXS data
James Byrnes
RgGuinier 8.1 nm
Dmax 29.8 nm
VolumePorod 1950 nm3

SASDWA7 – Dihydroneopterin aldolase from H.pylori (HpDHNA:Pterin)

Dihydroneopterin aldolase experimental SAS data
DAMMIN model
Sample: Dihydroneopterin aldolase tetramer, 55 kDa Helicobacter pylori (strain … protein
Buffer: 25 mM Tris-HCl pH 7.5 and 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Apr 5
Structure of Helicobacter pylori dihydroneopterin aldolase suggests a fragment-based strategy for isozyme-specific inhibitor design. Curr Res Struct Biol 5:100095 (2023)
Shaw GX, Fan L, Cherry S, Shi G, Tropea JE, Ji X
RgGuinier 2.5 nm
Dmax 7.3 nm
VolumePorod 77 nm3

SASDLG7 – LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94)

Relaxin receptor 1 experimental SAS data
LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94) Rg histogram
Sample: Relaxin receptor 1 monomer, 8 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Apr 6
Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2. J Mol Biol 433(21):167217 (2021)
Sethi A, Bruell S, Ryan T, Yan F, Tanipour MH, Mok YF, Draper-Joyce C, Khandokar Y, Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR
RgGuinier 2.1 nm
Dmax 10.0 nm
VolumePorod 19 nm3

SASDPG7 – ...WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 16 nm3

SASDQG7 – Thermoanearobacter tengcongensis (Tte) fecB RNA riboswitch aptamer domain (rCbl)

Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain experimental SAS data
OTHER model
Sample: Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain , 68 kDa marine metagenome RNA
Buffer: 50 mM MES pH 6.0, 10 mM KCl, 1 mM MgCl2, pH: 6
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Apr 18
Visualizing RNA conformational and architectural heterogeneity in solution. Nat Commun 14(1):714 (2023)
Ding J, Lee YT, Bhandari Y, Schwieters CD, Fan L, Yu P, Tarosov SG, Stagno JR, Ma B, Nussinov R, Rein A, Zhang J, Wang YX
RgGuinier 5.9 nm
Dmax 20.7 nm
VolumePorod 206 nm3

SASDCH7 – N-terminal domain of DsbD from N. meningitidis

N-terminus of disulfide interchange protein DsbD experimental SAS data
DAMMIN model
Sample: N-terminus of disulfide interchange protein DsbD monomer, 14 kDa Neisseria meningitidis protein
Buffer: 25mM HEPES 150mM NaCl, pH: 6.7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2013 May 4
Production, biophysical characterization and initial crystallization studies of the N- and C-terminal domains of DsbD, an essential enzyme in Neisseria meningitidis. Acta Crystallogr F Struct Biol Commun 74(Pt 1):31-38 (2018)
Smith RP, Whitten AE, Paxman JJ, Kahler CM, Scanlon MJ, Heras B
RgGuinier 1.8 nm
Dmax 5.7 nm
VolumePorod 17 nm3

SASDLH7 – LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105)

Relaxin receptor 2 experimental SAS data
LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105) Rg histogram
Sample: Relaxin receptor 2 monomer, 7 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Apr 6
Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2. J Mol Biol 433(21):167217 (2021)
Sethi A, Bruell S, Ryan T, Yan F, Tanipour MH, Mok YF, Draper-Joyce C, Khandokar Y, Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR
RgGuinier 1.7 nm
Dmax 7.1 nm
VolumePorod 12 nm3

SASDQH7 – Thermoanearobacter tengcongensis (Tte) fecB RNA riboswitch aptamer domain (rCbl) in the presence of coenzyme B12

Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain experimental SAS data
OTHER model
Sample: Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain , 68 kDa marine metagenome RNA
Buffer: 50 mM MES pH 6.0, 10 mM KCl, 1 mM MgCl2, 4-18 μM coenzyme B12 ligand, pH: 6
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Apr 18
Visualizing RNA conformational and architectural heterogeneity in solution. Nat Commun 14(1):714 (2023)
Ding J, Lee YT, Bhandari Y, Schwieters CD, Fan L, Yu P, Tarosov SG, Stagno JR, Ma B, Nussinov R, Rein A, Zhang J, Wang YX
RgGuinier 5.7 nm
Dmax 19.7 nm
VolumePorod 230 nm3

SASDCJ7 – C-terminal domain of DsbD from N. meningitidis

DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein) experimental SAS data
DAMMIN model
Sample: DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein) monomer, 21 kDa Wolbachia endosymbiont of … protein
Buffer: 25mM HEPES 150mM NaCl, pH: 6.7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2013 May 4
Production, biophysical characterization and initial crystallization studies of the N- and C-terminal domains of DsbD, an essential enzyme in Neisseria meningitidis. Acta Crystallogr F Struct Biol Commun 74(Pt 1):31-38 (2018)
Smith RP, Whitten AE, Paxman JJ, Kahler CM, Scanlon MJ, Heras B
RgGuinier 1.5 nm
Dmax 4.5 nm
VolumePorod 15 nm3

SASDPJ7 – ...WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 214 nm3

SASDJL7 – Oxidised fimbrial BcfH protein

Hypothetical exported protein experimental SAS data
CORAL model
Sample: Hypothetical exported protein trimer, 83 kDa Salmonella typhimurium (strain … protein
Buffer: 25 mM TRIS, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Aug 14
Salmonella enterica BcfH is a trimeric thioredoxin-like bifunctional enzyme with both thiol oxidase and disulfide isomerase activities. Antioxid Redox Signal (2021)
Subedi P, Paxman JJ, Wang G, Hor L, Hong Y, Verderosa AD, Whitten AE, Panjikar S, Santos-Martin CF, Martin JL, Totsika M, Heras B
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 93 nm3

SASDPL7 – ...WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.3 nm
Dmax 14.1 nm
VolumePorod 233 nm3

SASDVM7 – Mycobacterium tuberculosis Isocitrate Lyase 2

Isocitrate lyase 2 experimental SAS data
DAMMIN model
Sample: Isocitrate lyase 2 monomer, 87 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2023 Jun 27
The mechanism behind acetyl-CoA-mediated activation in Mycobacterium tuberculosis isocitrate lyase 2 and the contribution of its unique protein features to allostery and selectivity
Ashish Sethi
RgGuinier 5.0 nm
Dmax 19.5 nm
VolumePorod 507 nm3

SASDPN7 – ...WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.6 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDVN7 – Mycobacterium tuberculosis Isocitrate Lyase 2 mutant (Q635C, V708C, V734C)

Isocitrate lyase 2 (Q635C, V708C, V734C) experimental SAS data
DAMMIN model
Sample: Isocitrate lyase 2 (Q635C, V708C, V734C) monomer, 87 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2023 Jun 27
The mechanism behind acetyl-CoA-mediated activation in Mycobacterium tuberculosis isocitrate lyase 2 and the contribution of its unique protein features to allostery and selectivity
Ashish Sethi
RgGuinier 5.2 nm
Dmax 20.6 nm
VolumePorod 526 nm3

SASDVP7 – Mycobacterium tuberculosis Isocitrate Lyase 2 mutant (E640C, N731C, E733C)

Isocitrate lyase 2 (E640C, N731C, E733C) experimental SAS data
DAMMIN model
Sample: Isocitrate lyase 2 (E640C, N731C, E733C) monomer, 87 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2023 Jun 27
The mechanism behind acetyl-CoA-mediated activation in Mycobacterium tuberculosis isocitrate lyase 2 and the contribution of its unique protein features to allostery and selectivity
Ashish Sethi
RgGuinier 5.5 nm
Dmax 23.9 nm
VolumePorod 617 nm3

SASDSY7 – Karrikin insensitive 2, KAI2 protein (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 58 nm3

SASDSZ7 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.9 nm
Dmax 13.9 nm
VolumePorod 60 nm3

SASDC28 – Mature α-DsbA2 protein

DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein) experimental SAS data
DAMMIN model
Sample: DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein) trimer, 81 kDa Wolbachia endosymbiont of … protein
Buffer: 25 mM TRIS, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2014 Mar 29
The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase. Acta Crystallogr D Struct Biol 75(Pt 3):283-295 (2019)
Walden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 913 nm3

SASDS28 – Karrikin insensitive 2, KAI2 protein (aggregate peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 283 nm3

SASDC38 – Truncated α-DsbA2 protein

DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein) experimental SAS data
DAMMIN model
Sample: DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein) monomer, 21 kDa Wolbachia endosymbiont of … protein
Buffer: 25 mM TRIS, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2012 Feb 29
The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase. Acta Crystallogr D Struct Biol 75(Pt 3):283-295 (2019)
Walden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL
RgGuinier 1.9 nm
Dmax 6.3 nm
VolumePorod 275 nm3

SASDS38 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.9 nm
Dmax 20.0 nm
VolumePorod 345 nm3

SASDC48 – ScsC-ScsBalpha complex

DsbA-like proteinPutative metal resistance protein experimental SAS data
SASREF model
Sample: DsbA-like protein trimer, 74 kDa Proteus mirabilis protein
Putative metal resistance protein monomer, 30 kDa Proteus mirabilis protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 2
Disulfide isomerase activity of the dynamic, trimeric Proteus mirabilis ScsC protein is primed by the tandem immunoglobulin-fold domain of ScsB. J Biol Chem 293(16):5793-5805 (2018)
Furlong EJ, Choudhury HG, Kurth F, Duff AP, Whitten AE, Martin JL
RgGuinier 3.9 nm
Dmax 11.5 nm
VolumePorod 145 nm3

SASDS48 – Karrikin insensitive 2, KAI2 protein mutant S95A (monomer peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
RgGuinier 1.9 nm
Dmax 6.3 nm
VolumePorod 55 nm3

SASDS58 – Karrikin insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.1 nm
Dmax 7.2 nm
VolumePorod 61 nm3

SASDS68 – Karrikin insensitive 2, KAI2 protein mutant S95A (aggregate peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 171 nm3

SASDB78 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I)

Probable ATP-dependent RNA helicase DDX58 experimental SAS data
BUNCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2012 Apr 6
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.3 nm
Dmax 14.0 nm
VolumePorod 186 nm3

SASDS78 – Karrikin insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (aggregate peak)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.5 nm
Dmax 19.3 nm
VolumePorod 285 nm3

SASDB88 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus ADP-AlFx

Probable ATP-dependent RNA helicase DDX58 experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.2 nm
Dmax 15.6 nm
VolumePorod 190 nm3

SASDS88 – Karrikin insensitive 2, KAI2 protein mutant S95C (monomer peak)

Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) experimental SAS data
Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) Kratky plot
Sample: Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 1.9 nm
Dmax 5.7 nm
VolumePorod 45 nm3

SASDB98 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus bound 10mer hairpin dsRNA

Probable ATP-dependent RNA helicase DDX585´ppp 10mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 May 29
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.1 nm
Dmax 16.1 nm
VolumePorod 160 nm3

SASDS98 – Karrikin insensitive 2, KAI2 protein mutant S95C with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) experimental SAS data
Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) Kratky plot
Sample: Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 1.9 nm
Dmax 5.7 nm
VolumePorod 35 nm3

SASDBA8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus 10mer hairpin dsRNA /AMP-PNP

Probable ATP-dependent RNA helicase DDX585´ppp 10mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 May 28
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 163 nm3

SASDSA8 – Karrikin insensitive 2, KAI2 protein mutant H246Y (monomer peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data
Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot
Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 3.0 nm
Dmax 16.1 nm
VolumePorod 70 nm3

SASDBB8 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus 10mer hairpin dsRNA and ADP-AlFx

Probable ATP-dependent RNA helicase DDX585´ppp 10mer hairpin dsRNA experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.0 nm
Dmax 18.3 nm
VolumePorod 156 nm3

SASDSB8 – Karrikin insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data
Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot
Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.9 nm
Dmax 13.2 nm
VolumePorod 68 nm3

SASDAC8 – SDS hydrolase SdsA1

Pseudomonas aeruginosa SDS hydrolase SdsA1 experimental SAS data
CRYSOL model
Sample: Pseudomonas aeruginosa SDS hydrolase SdsA1 dimer, 145 kDa Pseudomonas aeruginosa protein
Buffer: 50 mM HEPES, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Jul 22
SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques Journal of Applied Crystallography 48(5):1551-1559 (2015)
De la Mora E, Flores-Hernández E, Jakoncic J, Stojanoff V, Siliqi D, Sánchez-Puig N, Moreno A
RgGuinier 3.6 nm
Dmax 16.9 nm
VolumePorod 261 nm3

SASDSC8 – Karrikin insensitive 2, KAI2 protein mutant H246Y (aggregate peak)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data
Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot
Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 5.6 nm
Dmax 23.0 nm
VolumePorod 494 nm3