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206 hits found for waxs

SASDMC2 – ...WAXS data from Lysozyme Solution [Protein concentration 5 mg/ml] at 10 °C

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM NaOAc pH 3.8, 150 mM NaCl, pH: 3.8
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 May 2
...WAXS data analysis improves shape restoration of biomolecules: case of lysozyme. J Biomol Struct Dyn :1-15 (2021)
Ashish
RgGuinier 1.3 nm
Dmax 4.2 nm

SASDKF2 – Mixed-sequence DNA dimer (25 base pair DNA)

Mixed-sequence 25 base-paired DNA experimental SAS data
Mixed-sequence 25 base-paired DNA Kratky plot
Sample: Mixed-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
...WAXS and MD. Sci Adv 7(17) (2021)
He W, Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 17 nm3

SASDAG2 – Lyz in Sodium Acetate

Lysozyme C experimental SAS data
GASBOR model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM Sodium Acetate, pH: 3.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 21
Standard proteins
Darja Ruskule
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 21 nm3

SASDGG2 – Interleukin 11 receptor subunit alpha

Interleukin-11 receptor subunit alpha experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 41 nm3

SASDKG2 – AT-sequence DNA dimer (25 base pair DNA)

AT-sequence 25 base-paired DNA experimental SAS data
AT-sequence 25 base-paired DNA Kratky plot
Sample: AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
...WAXS and MD. Sci Adv 7(17) (2021)
He W, Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 16 nm3

SASDGH2 – Interleukin 11, N-terminally truncated

Interleukin 11 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Interleukin 11 monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.7 nm
Dmax 5.4 nm
VolumePorod 22 nm3

SASDGJ2 – Interleukin 11, full length

Interleukin 11 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Interleukin 11 monomer, 19 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.9 nm
Dmax 6.1 nm
VolumePorod 28 nm3

SASDAK2 – Myo in PBS

Myoglobin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 20
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 32 nm3

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 229 nm3

SASDGK2 – Interleukin 11/Interleukin 11 receptor alpha complex

Interleukin-11 receptor subunit alphaInterleukin 11 experimental SAS data
OTHER model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Interleukin 11 monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 3.3 nm
Dmax 10.2 nm
VolumePorod 84 nm3

SASDJM2 – Flagellotropic bacteriophage YSD1 protein, YSD1_22 - Major Tail Protein

Major tail protein experimental SAS data
DAMFILT model
Sample: Major tail protein monomer, 40 kDa Salmonella virus Chi protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Mar 8
The architecture and stabilisation of flagellotropic tailed bacteriophages. Nat Commun 11(1):3748 (2020)
Hardy JM, Dunstan RA, Grinter R, Belousoff MJ, Wang J, Pickard D, Venugopal H, Dougan G, Lithgow T, Coulibaly F
RgGuinier 3.3 nm
Dmax 10.2 nm
VolumePorod 54 nm3

SASDAN2 – RNase in PBS

Ribonuclease pancreatic experimental SAS data
GASBOR model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 15 nm3

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

Calmodulin-1 experimental SAS data
4Ca2+-calmodulin - Xenopus laevis Rg histogram
Sample: Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 2.2 nm
Dmax 7.2 nm
VolumePorod 25 nm3

SASDHQ2 – ...WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 100 mM NaCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.1 nm

SASDHR2 – ...WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 10.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.6 nm

SASDHS2 – ...WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.3 nm

SASDHY2 – Human APPL2 (DCC-interacting protein 13-beta)

Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 experimental SAS data
BUNCH model
Sample: Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 dimer, 87 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 7 Apr 20
Membrane curvature protein exhibits interdomain flexibility and binds a small GTPase. J Biol Chem 287(49):40996-1006 (2012)
King GJ, Stöckli J, Hu SH, Winnen B, Duprez WG, Meoli CC, Junutula JR, Jarrott RJ, James DE, Whitten AE, Martin JL
RgGuinier 5.1 nm
Dmax 18.0 nm
VolumePorod 140 nm3

SASDHZ2 – 14-3-3 protein beta isoform

14-3-3 protein beta/alpha experimental SAS data
BUNCH model
Sample: 14-3-3 protein beta/alpha dimer, 58 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2011 Apr 7
The weak complex between RhoGAP protein ARHGAP22 and signal regulatory protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PLoS One 7(8):e41731 (2012)
Hu SH, Whitten AE, King GJ, Jones A, Rowland AF, James DE, Martin JL
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 92 nm3

SASDH23 – Antigen 43 alpha domain

Alpha domain of Ag43a experimental SAS data
DAMMIN model
Sample: Alpha domain of Ag43a monomer, 49 kDa Escherichia coli protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2009 Nov 19
The antigen 43 structure reveals a molecular Velcro-like mechanism of autotransporter-mediated bacterial clumping. Proc Natl Acad Sci U S A 111(1):457-62 (2014)
Heras B, Totsika M, Peters KM, Paxman JJ, Gee CL, Jarrott RJ, Perugini MA, Whitten AE, Schembri MA
RgGuinier 3.6 nm
Dmax 12.2 nm
VolumePorod 62 nm3

SASDH33 – Rho GTPase-activating protein 22

Rho GTPase-activating protein 22 experimental SAS data
BUNCH model
Sample: Rho GTPase-activating protein 22 monomer, 47 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2011 Apr 7
The weak complex between RhoGAP protein ARHGAP22 and signal regulatory protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PLoS One 7(8):e41731 (2012)
Hu SH, Whitten AE, King GJ, Jones A, Rowland AF, James DE, Martin JL
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 88 nm3

SASDC63 – Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide (FPV039:BAK)

Bcl-2-like protein FPV039Uncharacterized protein (BAK1) experimental SAS data
Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) Kratky plot
Sample: Bcl-2-like protein FPV039 monomer, 17 kDa Fowlpox virus protein
Uncharacterized protein (BAK1) monomer, 3 kDa Gallus gallus protein
Buffer: 20 mM trisodium citrate pH, 200 mM NaCl, pH: 6
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Oct 2
Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039. J Biol Chem 292(22):9010-9021 (2017)
Anasir MI, Caria S, Skinner MA, Kvansakul M
RgGuinier 2.0 nm

SASDD83 – Apolipoprotein D (ApoD) tetramer

Apolipoprotein D experimental SAS data
SASREF CV model
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Identification of a novel tetrameric structure for human apolipoprotein-D. J Struct Biol 203(3):205-218 (2018)
Kielkopf CS, Low JKK, Mok YF, Bhatia S, Palasovski T, Oakley AJ, Whitten AE, Garner B, Brown SHJ
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 169 nm3

SASDH83 – Chemically crossed linked complex between Rho GTPase-activating protein 22 (ARHGAP22) and the beta isoform of the 14-3-3 protein beta/alpha

14-3-3 protein beta/alphaRho GTPase-activating protein 22 experimental SAS data
CORAL model
Sample: 14-3-3 protein beta/alpha dimer, 58 kDa Homo sapiens protein
Rho GTPase-activating protein 22 monomer, 47 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2011 Apr 7
The weak complex between RhoGAP protein ARHGAP22 and signal regulatory protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PLoS One 7(8):e41731 (2012)
Hu SH, Whitten AE, King GJ, Jones A, Rowland AF, James DE, Martin JL
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 195 nm3

SASDGF3 – Xrn1 resistance RNA1 from Zika virus

Xrn1 resistance RNA2 from Zika virus experimental SAS data
OTHER model
Sample: Xrn1 resistance RNA2 from Zika virus monomer, 22 kDa Zika virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 34 nm3

SASDGH3 – Xrn1 resistance RNA1-2 from Zika virus

Xrn1 resistance RNA1-2 from Dengue virus 2 experimental SAS data
DAMMIN model
Sample: Xrn1 resistance RNA1-2 from Dengue virus 2 monomer, 46 kDa Dengue virus 2 RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Apr 2
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 3.9 nm
Dmax 13.4 nm
VolumePorod 67 nm3

SASDNH3 – HIV-1 reverse transcriptase L289K mutation at 1 mg/mL

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) experimental SAS data
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Kratky plot
Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20
Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 91 nm3

SASDCJ3 – Bovine Serum Albumin

Serum albumin experimental SAS data
DAMMIN model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 101 nm3

SASDGJ3 – Xrn1 resistance RNA1 from West Nile virus

Xrn1 resistance RNA-1 from West Nile virus experimental SAS data
DAMFILT model
Sample: Xrn1 resistance RNA-1 from West Nile virus monomer, 25 kDa West Nile virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 2.4 nm
Dmax 8.4 nm
VolumePorod 34 nm3

SASDNJ3 – HIV-1 reverse transcriptase L289K mutation at 2 mg/mL

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) experimental SAS data
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Kratky plot
Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20
Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R
RgGuinier 3.2 nm
Dmax 10.8 nm
VolumePorod 91 nm3

SASDFK3 – Splicing factor, proline- and glutamine-rich (SFPQ)

Splicing factor, proline- and glutamine-rich experimental SAS data
CORAL model
Sample: Splicing factor, proline- and glutamine-rich dimer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 250 mM NaCl, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Apr 19
A new crystal structure and small-angle X-ray scattering analysis of the homodimer of human SFPQ. Acta Crystallogr F Struct Biol Commun 75(Pt 6):439-449 (2019)
Hewage TW, Caria S, Lee M
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 91 nm3

SASDGK3 – Xrn1 resistance RNA1 from Murray Valley Encephalitis

Xrn1 resistance RNA-1 from Murray Valley Encephalitis experimental SAS data
DAMFILT model
Sample: Xrn1 resistance RNA-1 from Murray Valley Encephalitis monomer, 26 kDa Murray Valley Encephalitis RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 2.4 nm
Dmax 8.8 nm
VolumePorod 31 nm3

SASDNK3 – HIV-1 reverse transcriptase L289K mutation at 4.3 mg/mL

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) experimental SAS data
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Kratky plot
Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20
Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 93 nm3

SASDBL3 – Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens

cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 experimental SAS data
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Kratky plot
Sample: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 monomer, 46 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 10
In vivo epidermal migration requires focal adhesion targeting of ACF7. Nat Commun 7:11692 (2016)
Yue J, Zhang Y, Liang WG, Gou X, Lee P, Liu H, Lyu W, Tang WJ, Chen SY, Yang F, Liang H, Wu X
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 53 nm3

SASDGL3 – 3'SL from Dengue virus 2

3'SL from Dengue virus 2 experimental SAS data
DAMFILT model
Sample: 3'SL from Dengue virus 2 monomer, 31 kDa Dengue virus 2 RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Feb 24
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 3.6 nm
Dmax 14.1 nm
VolumePorod 40 nm3

SASDNL3 – HIV-1 reverse transcriptase L289K mutation (extrapolated to zero concentration)

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) experimental SAS data
BUNCH model
Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 20
Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 90 nm3

SASDGM3 – Xrn1 resistance RNA1-2 from Dengue virus 2

Xrn1 resistance RNA1-2 from Dengue virus 2 experimental SAS data
DAMFILT model
Sample: Xrn1 resistance RNA1-2 from Dengue virus 2 monomer, 46 kDa Dengue virus 2 RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 3.5 nm
Dmax 12.5 nm
VolumePorod 65 nm3

SASDBN3 – Highly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens

cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant experimental SAS data
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant Kratky plot
Sample: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant monomer, 46 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 7
In vivo epidermal migration requires focal adhesion targeting of ACF7. Nat Commun 7:11692 (2016)
Yue J, Zhang Y, Liang WG, Gou X, Lee P, Liu H, Lyu W, Tang WJ, Chen SY, Yang F, Liang H, Wu X
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 55 nm3

SASDGP3 – Xrn1 resistance RNA1-2 from West Nile virus

Xrn1 resistance RNA1-2 from West Nile virus experimental SAS data
DAMFILT model
Sample: Xrn1 resistance RNA1-2 from West Nile virus monomer, 75 kDa West Nile virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Apr 2
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 4.7 nm
Dmax 17.4 nm
VolumePorod 235 nm3

SASDKP3 – Alpha domain of autotransporter adhesin Ag43a from Escherichia coli strain UTI89 / UPEC (alpha-Ag43_UTI)

Putative autotransporter experimental SAS data
Sample: Putative autotransporter dimer, 119 kDa Escherichia coli (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 May 1
Variation of Antigen 43 self-association modulates bacterial compacting within aggregates and biofilms npj Biofilms and Microbiomes 8(1) (2022)
Vo J, Ortiz G, Totsika M, Lo A, Hancock S, Whitten A, Hor L, Peters K, Ageorges V, Caccia N, Desvaux M, Schembri M, Paxman J, Heras B
RgGuinier 3.7 nm
Dmax 13.5 nm
VolumePorod 79 nm3

SASDKQ3 – Alpha domain of autotransporter adhesin Ag43a from Escherichia coli

Alpha domain of Ag43a experimental SAS data
Sample: Alpha domain of Ag43a monomer, 49 kDa Escherichia coli protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 3
Variation of Antigen 43 self-association modulates bacterial compacting within aggregates and biofilms npj Biofilms and Microbiomes 8(1) (2022)
Vo J, Ortiz G, Totsika M, Lo A, Hancock S, Whitten A, Hor L, Peters K, Ageorges V, Caccia N, Desvaux M, Schembri M, Paxman J, Heras B
RgGuinier 4.6 nm
Dmax 18.0 nm
VolumePorod 87 nm3

SASDGR3 – Xrn1 resistance RNA2 from Dengue virus 2

Xrn1 resistance RNA2 from Dengue virus 2 experimental SAS data
DAMFILT model
Sample: Xrn1 resistance RNA2 from Dengue virus 2 monomer, 21 kDa Dengue virus 2 RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Mar 16
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 29 nm3

SASDFS3 – Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D

Transcription intermediary factor 1-beta experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta dimer, 82 kDa Mus musculus protein
Buffer: 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Aug 10
A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. J Mol Biol (2019)
Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC
RgGuinier 7.0 nm
Dmax 23.2 nm
VolumePorod 232 nm3

SASDGS3 – DB12 from Dengue virus 2

DB12 from Dengue virus 2 experimental SAS data
DAMFILT model
Sample: DB12 from Dengue virus 2 monomer, 56 kDa Dengue virus 2 RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Feb 24
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 4.7 nm
Dmax 16.2 nm
VolumePorod 96 nm3

SASDFT3 – Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D, complexed with the Krab domain of ZFP809 fused to an N-terminal MBP

Transcription intermediary factor 1-betaZinc finger protein 809 N-terminal MBP fusion experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta dimer, 82 kDa Mus musculus protein
Zinc finger protein 809 N-terminal MBP fusion monomer, 52 kDa Mus musculus protein
Buffer: 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Aug 10
A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. J Mol Biol (2019)
Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC
RgGuinier 6.4 nm
Dmax 22.0 nm
VolumePorod 252 nm3

SASDGU3 – Xrn1 resistance RNA2 from Murray Valley Encephalitis

Xrn1 resistance RNA2 from Murray Valley Encephalitis experimental SAS data
DAMMIF model
Sample: Xrn1 resistance RNA2 from Murray Valley Encephalitis monomer, 22 kDa Murray Valley Encephalitis RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 14
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 2.2 nm
Dmax 8.2 nm
VolumePorod 28 nm3

SASDGV3 – DB12 from Zika virus

DB12 from Zika virus experimental SAS data
DAMFILT model
Sample: DB12 from Zika virus monomer, 47 kDa Zika virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Apr 2
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 3.3 nm
Dmax 11.2 nm
VolumePorod 92 nm3

SASDGW3 – DB12 from West Nile virus

DB12 from West Nile virus experimental SAS data
DAMFILT model
Sample: DB12 from West Nile virus monomer, 59 kDa West Nile virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jan 19
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 3.9 nm
Dmax 13.4 nm
VolumePorod 160 nm3

SASDJW3 – YraP from Acinetobacter baumannii full-length, minus signal-peptide and acyl anchoring cysteine

BON domain protein experimental SAS data
DAMMIF model
Sample: BON domain protein decamer, 229 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 11
BonA from Acinetobacter baumannii Forms a Divisome-Localized Decamer That Supports Outer Envelope Function. mBio :e0148021 (2021)
Grinter R, Morris FC, Dunstan RA, Leung PM, Kropp A, Belousoff M, Gunasinghe SD, Scott NE, Beckham S, Peleg AY, Greening C, Li J, Heinz E, Lithgow T
RgGuinier 4.7 nm
Dmax 16.4 nm
VolumePorod 546 nm3

SASDGX3 – 3'SL from Zika virus

3'SL from Zika virus experimental SAS data
DAMFILT model
Sample: 3'SL from Zika virus monomer, 32 kDa Zika virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 52 nm3

SASDJX3 – YraP from Acinetobacter baumannii full-length, minus signal-peptide and 27 N-terminal amino acids

BON domain protein experimental SAS data
DAMMIF model
Sample: BON domain protein monomer, 20 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 11
BonA from Acinetobacter baumannii Forms a Divisome-Localized Decamer That Supports Outer Envelope Function. mBio :e0148021 (2021)
Grinter R, Morris FC, Dunstan RA, Leung PM, Kropp A, Belousoff M, Gunasinghe SD, Scott NE, Beckham S, Peleg AY, Greening C, Li J, Heinz E, Lithgow T
RgGuinier 3.1 nm
Dmax 10.8 nm
VolumePorod 49 nm3

SASDFZ3 – Escherichia coli YjhC

Escherichia coli YjhC experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Escherichia coli YjhC dimer, 86 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, 5 % (v/v) glycerol, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Apr 12
On the structure and function of Escherichia coli YjhC: an oxidoreductase involved in bacterial sialic acid metabolism. Proteins (2019)
Horne CR, Kind L, Davies JS, Dobson RCJ
RgGuinier 3.1 nm
Dmax 10.7 nm
VolumePorod 130 nm3

SASDGZ3 – Subgenomic flavivirus RNAs from Zika virus

Subgenomic flavivirus RNA from Zika virus experimental SAS data
Subgenomic flavivirus RNAs from Zika virus Rg histogram
Sample: Subgenomic flavivirus RNA from Zika virus monomer, 133 kDa Zika virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Mar 13
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 7.5 nm
Dmax 29.6 nm
VolumePorod 230 nm3

SASDG24 – Subgenomic flavivirus RNAs from Dengue virus 2

Subgenomic flavivirus RNAs from Dengue virus 2 experimental SAS data
Subgenomic flavivirus RNAs from Dengue virus 2 Rg histogram
Sample: Subgenomic flavivirus RNAs from Dengue virus 2 monomer, 137 kDa Dengue virus 2 RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 16
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 7.9 nm
Dmax 30.5 nm
VolumePorod 236 nm3

SASDH24 – Calcium-Activated Chloride Channel Regulator 1 VWA domain

Calcium-activated chloride channel regulator 1 experimental SAS data
DAMFILT model
Sample: Calcium-activated chloride channel regulator 1 monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jun 6
Structural and Biophysical Analysis of the CLCA1 VWA Domain Suggests Mode of TMEM16A Engagement. Cell Rep 30(4):1141-1151.e3 (2020)
Berry KN, Brett TJ
RgGuinier 1.8 nm
Dmax 6.6 nm

SASDG34 – Subgenomic flavivirus RNAs from West nile virus

Subgenomic flavivirus RNAs from West nile virus experimental SAS data
Subgenomic flavivirus RNAs from West nile virus Rg histogram
Sample: Subgenomic flavivirus RNAs from West nile virus monomer, 170 kDa West Nile virus RNA
Buffer: 20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Mar 13
Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Rep 20(11):e47016 (2019)
Zhang Y, Zhang Y, Liu ZY, Cheng ML, Ma J, Wang Y, Qin CF, Fang X
RgGuinier 7.9 nm
Dmax 31.8 nm
VolumePorod 261 nm3

SASDG54 – Human macrophage mannose receptor 1 protein

Macrophage mannose receptor 1 experimental SAS data
ITASSER model
Sample: Macrophage mannose receptor 1 dimer, 315 kDa Mouse myeloma cell … protein
Buffer: 50mM Hepes, 100mM NaCl, 1mM DTT, pH: 7
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 15
Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses. Sci Transl Med 12(530) (2020)
Jaynes JM, Sable R, Ronzetti M, Bautista W, Knotts Z, Abisoye-Ogunniyan A, Li D, Calvo R, Dashnyam M, Singh A, Guerin T, White J, Ravichandran S, Kumar P, Talsania K, Chen V, Ghebremedhin A, Karanam B...
RgGuinier 7.9 nm
Dmax 30.1 nm
VolumePorod 584 nm3

SASDB94 – Suppressor of Copper Sensitivity C protein (ScsC) from Proteus mirabilis

C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein experimental SAS data
Suppressor of Copper Sensitivity C protein (ScsC) from Proteus mirabilis Rg histogram
Sample: C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein monomer, 20 kDa Proteus mirabilis protein
Buffer: 25 mM HEPES 150mM NaCl 1mM DTT, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2012 Feb 29
A shape-shifting redox foldase contributes to Proteus mirabilis copper resistance. Nat Commun 8:16065 (2017)
Furlong EJ, Lo AW, Kurth F, Premkumar L, Totsika M, Achard MES, Halili MA, Heras B, Whitten AE, Choudhury HG, Schembri MA, Martin JL
RgGuinier 3.7 nm
Dmax 10.5 nm
VolumePorod 92 nm3

SASDKK4 – A-type ATP synthase

n-Dodecyl-β-D-MaltopyranosideA-type ATP synthaseMonoclonal Antibody Fragment experimental SAS data
OTHER model
Sample: n-Dodecyl-β-D-Maltopyranoside , 77 kDa synthetic construct
A-type ATP synthase monomer, 655 kDa Thermus thermophilus protein
Monoclonal Antibody Fragment dimer, 33 kDa Homo sapiens protein
Buffer: 20 mM Tris/HCl, 100 mM sucrose, 100 mM NaCl, 2 mM MgCl2, 10% glycerol, 0.05% b-DDM, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2013 Mar 3
MPBuilder: A PyMOL Plugin for Building and Refinement of Solubilized Membrane Proteins Against Small Angle X-ray Scattering Data Journal of Molecular Biology :166888 (2021)
Molodenskiy D, Svergun D, Mertens H
RgGuinier 8.0 nm
Dmax 27.0 nm
VolumePorod 1549 nm3

SASDDQ4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) of Insulin gene enhancer protein ISL-2

LIM/homeobox protein Lhx4Insulin gene enhancer protein ISL-2 experimental SAS data
DAMMIN model
Sample: LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein
Insulin gene enhancer protein ISL-2 monomer, 4 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Nov 19
Mutation in a flexible linker modulates binding affinity for modular complexes. Proteins (2019)
Stokes PH, Robertson NO, Silva AP, Estephan T, Trewhella J, Guss JM, Matthews JM
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 20 nm3

SASDMQ4 – Hepatitis C virus RNA Domain 3'X mutant

Domain 3'X mutant experimental SAS data
DAMFILT model
Sample: Domain 3'X mutant monomer, 32 kDa Hepatitis C virus … RNA
Buffer: 10 mM Tris-HCl, 6 mM MgCl2, pH: 7
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 2
The low-resolution structural models of hepatitis C virus RNA subdomain 5BSL3.2 and its distal complex with domain 3'X point to conserved regulatory mechanisms within the Flaviviridae family. Nucleic Acids Res (2022)
Castillo-Martínez J, Fan L, Szewczyk MP, Wang YX, Gallego J
RgGuinier 3.3 nm
Dmax 14.2 nm
VolumePorod 50 nm3

SASDDR4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) R282G mutant of Insulin gene enhancer protein ISL-2

LIM/homeobox protein Lhx4Insulin gene enhancer protein ISL-2 (R282G) experimental SAS data
DAMMIN model
Sample: LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein
Insulin gene enhancer protein ISL-2 (R282G) monomer, 4 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Nov 19
Mutation in a flexible linker modulates binding affinity for modular complexes. Proteins (2019)
Stokes PH, Robertson NO, Silva AP, Estephan T, Trewhella J, Guss JM, Matthews JM
RgGuinier 2.3 nm
Dmax 8.5 nm
VolumePorod 21 nm3

SASDBV4 – Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein

MVA F1L antiapoptotic Bcl-2 viral protein experimental SAS data
BUNCH model
Sample: MVA F1L antiapoptotic Bcl-2 viral protein dimer, 51 kDa Vaccinia virus protein
Buffer: 25 mM HEPES 150 mM NaCl 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Apr 15
The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation. J Biol Chem 291(28):14600-8 (2016)
Caria S, Marshall B, Burton RL, Campbell S, Pantaki-Eimany D, Hawkins CJ, Barry M, Kvansakul M
RgGuinier 3.4 nm
Dmax 16.2 nm
VolumePorod 74 nm3

SASDB25 – Cardiac myosin binding protein-C: domains C5-C6-C7

Cardiac myosin binding protein-C: domains C5-C6-C7 experimental SAS data
Cardiac myosin binding protein-C: domains C5-C6-C7 Kratky plot
Sample: Cardiac myosin binding protein-C: domains C5-C6-C7 monomer, 36 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 250 mM NaCl, 2 mM TCEP, 0.02% sodium azide, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 Apr 18
Clinically Linked Mutations in the Central Domains of Cardiac Myosin-Binding Protein C with Distinct Phenotypes Show Differential Structural Effects. Structure 24(1):105-115 (2016)
Nadvi NA, Michie KA, Kwan AH, Guss JM, Trewhella J
RgGuinier 3.8 nm
Dmax 14.1 nm
VolumePorod 55 nm3

SASDC45 – Alpha domain of autotransporter protein UpaB from UPEC

Alpha domain of autotransporter protein UpaB experimental SAS data
CORAL model
Sample: Alpha domain of autotransporter protein UpaB monomer, 48 kDa E. Coli CFT073 protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2015 May 1
Unique structural features of a bacterial autotransporter adhesin suggest mechanisms for interaction with host macromolecules. Nat Commun 10(1):1967 (2019)
Paxman JJ, Lo AW, Sullivan MJ, Panjikar S, Kuiper M, Whitten AE, Wang G, Luan CH, Moriel DG, Tan L, Peters KM, Phan MD, Gee CL, Ulett GC, Schembri MA, Heras B
RgGuinier 2.9 nm
Dmax 10.5 nm
VolumePorod 66 nm3

SASDH55 – Modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398 experimental SAS data
modified stem loop IV poliovirus IRES, nucleotides 278-398 Kratky plot
Sample: modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Dec 2
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 94 nm3

SASDH65 – Poly(rC)-binding protein 2 (PCBP2)

Poly(rC)-binding protein 2 experimental SAS data
Poly(rC)-binding protein 2 Kratky plot
Sample: Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Jul 16
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.3 nm
Dmax 13.2 nm
VolumePorod 76 nm3

SASDH75 – Poly(rC)-binding protein 2 (PCBP2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm) - PCBP2/SLIVm complex

Poly(rC)-binding protein 2modified stem loop IV poliovirus IRES, nucleotides 278-398 experimental SAS data
Poly(rC)-binding protein 2 modified stem loop IV poliovirus IRES, nucleotides 278-398 Kratky plot
Sample: Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein
modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Dec 16
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.7 nm
Dmax 11.5 nm
VolumePorod 162 nm3

SASDH85 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH3) with bound modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398Truncated poly(rC)-binding protein 2 (ΔKH3)Truncated poly(rC)-binding protein 2 (ΔKH3) experimental SAS data
modified stem loop IV poliovirus IRES, nucleotides 278-398 Truncated poly(rC)-binding protein 2 (ΔKH3) Truncated poly(rC)-binding protein 2 (ΔKH3) Kratky plot
Sample: modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Jul 1
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.8 nm
Dmax 12.2 nm
VolumePorod 165 nm3

SASDJ85 – Zinc transporter 1 – hZnT1-CTD

Zinc transporter 1 experimental SAS data
DAMFILT model
Sample: Zinc transporter 1 dimer, 37 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2019 Aug 14
Heterologous Expression and Biochemical Characterization of the Human Zinc Transporter 1 (ZnT1) and Its Soluble C-Terminal Domain Frontiers in Chemistry 9 (2021)
Cotrim C, Jarrott R, Whitten A, Choudhury H, Drew D, Martin J
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 50 nm3

SASDH95 – Truncated Poly(rC)-binding protein 2 (PCBP2-ΔKH3)

Truncated poly(rC)-binding protein 2 (ΔKH3) experimental SAS data
Truncated poly(rC)-binding protein 2 (ΔKH3) Kratky plot
Sample: Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Jan 1
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 2.6 nm
Dmax 9.2 nm
VolumePorod 66 nm3

SASDHA5 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH1-KH2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) experimental SAS data
modified stem loop IV poliovirus IRES, nucleotides 278-398 Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) Kratky plot
Sample: modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) monomer, 18 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Jul 1
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 126 nm3

SASDHJ5 – Apolipoprotein-D plus biliverdin

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 168 nm3

SASDHK5 – Apolipoprotein-D oxidised with H2O2

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.3 nm
VolumePorod 164 nm3

SASDJK5 – Nucleolysin TIA-1 isoform p40 (TIA-1) bound to TC1 DNA

Nucleolysin TIA-1 isoform p40TC1 experimental SAS data
Nucleolysin TIA-1 isoform p40 TC1 Kratky plot
Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
TC1 monomer, 3 kDa synthetic construct DNA
Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Jun 26
Tandem RNA binding sites induce self-association of the stress granule marker protein TIA-1. Nucleic Acids Res (2021)
Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA
RgGuinier 3.2 nm
Dmax 15.1 nm
VolumePorod 78 nm3

SASDHL5 – Apolipoprotein-D plus palmitic acid

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.6 nm
VolumePorod 163 nm3

SASDJL5 – Nucleolysin TIA-1 isoform p40 (TIA-1) bound to UC1 RNA

Nucleolysin TIA-1 isoform p40UC1 experimental SAS data
Nucleolysin TIA-1 isoform p40 UC1 Kratky plot
Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
UC1 monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Jun 26
Tandem RNA binding sites induce self-association of the stress granule marker protein TIA-1. Nucleic Acids Res (2021)
Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA
RgGuinier 3.3 nm
Dmax 14.4 nm
VolumePorod 77 nm3

SASDHM5 – Apolipoprotein-D plus progesterone

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.0 nm
VolumePorod 161 nm3

SASDJM5 – Modified nucleolysin TIA-1 isoform p40 (TIA-1 APO)

Modified Nucleolysin TIA-1 isofrom p40 experimental SAS data
Modified Nucleolysin TIA-1 isofrom p40 Kratky plot
Sample: Modified Nucleolysin TIA-1 isofrom p40 monomer, 42 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 60 mM KCl, 0.5 M arginine-HCl, 1 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2020 Jul 7
Tandem RNA binding sites induce self-association of the stress granule marker protein TIA-1. Nucleic Acids Res (2021)
Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA
RgGuinier 3.2 nm
Dmax 12.7 nm
VolumePorod 62 nm3

SASDHN5 – Apolipoprotein-D plus sphingomyelin

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 9.9 nm
VolumePorod 171 nm3

SASDKP5 – RNA Short Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDHQ5 – Ile-Leu-Gln-Ile-Asn-Ser (ILQINS) hexapeptide self-assembly

Ile-Leu-Gln-Ile-Asn-Ser peptide experimental SAS data
OTHER model
Sample: Ile-Leu-Gln-Ile-Asn-Ser peptide , 1 kDa synthetic construct protein
Buffer: pure (MQ, 18 MΩ) Water, pH: 7
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2018 Nov 1
Amyloid Evolution: Antiparallel Replaced by Parallel. Biophys J (2020)
Zanjani AAH, Reynolds NP, Zhang A, Schilling T, Mezzenga R, Berryman JT

SASDKQ5 – RNA Short Tetraloop Hairpin Duplex in 200 mM KCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.0 nm
Dmax 6.8 nm

SASDKR5 – RNA Short Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.1 nm
Dmax 7.8 nm

SASDKS5 – RNA Short Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDKT5 – RNA Short Tetraloop Hairpin Triplex in 200 mM KCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.6 nm

SASDKU5 – RNA Short Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm

SASDDV5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum

4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 159 nm3

SASDKV5 – RNA Long Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 9.8 nm

SASDDW5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum + pyruvate

4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, 5mM sodium pyruvate, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.3 nm
Dmax 8.9 nm
VolumePorod 165 nm3

SASDKW5 – RNA Long Tetraloop Hairpin Duplex in 200 mM KCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.0 nm

SASDKX5 – RNA Long Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.3 nm

SASDKY5 – RNA Long Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 10.3 nm

SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDK26 – RNA Long Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
...WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 18.2 nm

SASDD66 – Phox homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha experimental SAS data
DAMFILT model
Sample: Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha monomer, 33 kDa Homo sapiens protein
Buffer: 25 mM Tris 200 mM NaCl 5% Glycerol 0.5 mM TCEP, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 20
Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α. Structure (2018)
Chen KE, Tillu VA, Chandra M, Collins BM
RgGuinier 2.6 nm
Dmax 9.3 nm
VolumePorod 43 nm3

SASDC76 – Extracellular domain of human B-lymphocyte cell receptor CD22

CD22 extracellular domain experimental SAS data
DAMMIF model
Sample: CD22 extracellular domain monomer, 90 kDa Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 9
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 21
Molecular basis of human CD22 function and therapeutic targeting. Nat Commun 8(1):764 (2017)
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
RgGuinier 8.0 nm
Dmax 30.6 nm

SASDD76 – Phox Homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α) in complex with inositol-hexaphosphate (IP6)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha experimental SAS data
DAMFILT model
Sample: Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha monomer, 33 kDa Homo sapiens protein
Buffer: 25 mM Tris 200 mM NaCl 5% Glycerol 0.5 mM TCEP 4 mM InsP6, pH: 8.5
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Oct 20
Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α. Structure (2018)
Chen KE, Tillu VA, Chandra M, Collins BM
RgGuinier 2.6 nm
Dmax 9.3 nm
VolumePorod 48 nm3

SASDC86 – Extracellular domain of human B-lymphocyte cell receptor CD22 in complex with alpha 2,6 sialyllactose

CD22 extracellular domainalpha(2,6)-Sialyllactose experimental SAS data
DAMMIF model
Sample: CD22 extracellular domain monomer, 90 kDa Homo sapiens protein
alpha(2,6)-Sialyllactose , 1 kDa
Buffer: 20 mM Tris 150 mM NaCl, pH: 9
Experiment: SAXS data collected at ...WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 21
Molecular basis of human CD22 function and therapeutic targeting. Nat Commun 8(1):764 (2017)
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
RgGuinier 8.1 nm
Dmax 29.8 nm

SASDEA6 – Staphylococcus aureus N-acetylglucosamine-6-phosphate deacetylase dimer

N-acetylglucosamine-6-phosphate deacetylase experimental SAS data
PYMOL model
Sample: N-acetylglucosamine-6-phosphate deacetylase dimer, 86 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris-HCl 150 mM NaCl, pH: 8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2016 Apr 26
Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus. FEBS Lett (2018)
Davies JS, Coombes D, Horne CR, Pearce FG, Friemann R, North RA, Dobson RCJ
RgGuinier 3.2 nm
Dmax 10.1 nm
VolumePorod 107 nm3

SASDFA6 – Proteolytic fragment of phage flagella binding tail protein YSD1_29 (amino acids 373-1296)

Flagella binding tail protein experimental SAS data
DAMMIF model
Sample: Flagella binding tail protein monomer, 103 kDa Salmonella virus Chi protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at ...WAXS, Australian Synchrotron on 2017 Apr 4
The flagellotropic bacteriophage YSD1 targets Salmonella Typhi with a Chi-like protein tail fibre. Mol Microbiol (2019)
Dunstan RA, Pickard D, Dougan S, Goulding D, Cormie C, Hardy J, Li F, Grinter R, Harcourt K, Yu L, Song J, Schreiber F, Choudhary J, Clare S, Coulibaly F, Strugnell RA, Dougan G, Lithgow T
RgGuinier 5.6 nm
Dmax 27.4 nm
VolumePorod 155 nm3