Small Angle Scattering Biological Data Bank SASBDB


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352 hits found for X33

SASDMV4 – Full-length Retinoic acid receptor RXR-alpha in complex with Ramp2 DNA

Retinoic acid receptor RXR-alphaRamp2 DNA experimental SAS data

Sample:	Retinoic acid receptor RXR-alpha dimer, 102 kDa Homo sapiens protein Ramp2 DNA monomer, 11 kDa DNA
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 May 7

Solution Behavior of the Intrinsically Disordered N-Terminal Domain of Retinoid X Receptor α in the Context of the Full-Length Protein Biochemistry 55(12):1741-1748 (2016)
Belorusova A, Osz J, Petoukhov M, Peluso-Iltis C, Kieffer B, Svergun D, Rochel N

R_g^Guinier	4.4	nm
D_max	13.9	nm
Volume^Porod	161	nm³

SASDAW4 – uPAR wild-type AE234 complex

Urokinase plasminogen activator surface receptorsynthetic peptide AE234 experimental SAS data

Urokinase plasminogen activator surface receptor synthetic peptide AE234 Kratky plot

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein synthetic peptide AE234 monomer, 1 kDa protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Jun 10

A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M

R_g^Guinier	2.4	nm
D_max	8.3	nm
Volume^Porod	57	nm³

SASDMW4 – Retinoic acid receptor RXR-alpha N-terminal and DNA binding domains (DBD) in complex with Ramp2 DNA

Ramp2 DNARetinoic acid receptor RXR-alpha experimental SAS data

Sample:	Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 44 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Jul 17

R_g^Guinier	4.3	nm
D_max	14.3	nm
Volume^Porod	89	nm³

SASDAX4 – uPAR wild-type ATF complex

Urokinase plasminogen activator surface receptorUrokinase-type plasminogen activator experimental SAS data

Urokinase plasminogen activator surface receptor Urokinase-type plasminogen activator Kratky plot

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Urokinase-type plasminogen activator monomer, 49 kDa Homo sapiens protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Jun 10

R_g^Guinier	2.8	nm
D_max	9.4	nm
Volume^Porod	102	nm³

SASDLX4 – Full-length flavorubredoxin

Anaerobic nitric oxide reductase flavorubredoxin experimental SAS data

Full-length flavorubredoxin Rg histogram

Sample:	Anaerobic nitric oxide reductase flavorubredoxin tetramer, 217 kDa Escherichia coli (strain … protein
Buffer:	50 mM Tris-HCl, 18% glycerol, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2006 Nov 19

Quaternary Structure of Flavorubredoxin as Revealed by Synchrotron Radiation Small-Angle X-Ray Scattering Structure 16(9):1428-1436 (2008)
Petoukhov M, Vicente J, Crowley P, Carrondo M, Teixeira M, Svergun D

R_g^Guinier	4.1	nm
D_max	14.2	nm
Volume^Porod	297	nm³

SASDMX4 – Retinoic acid receptor RXR-alpha DNA binding (DBD) and ligand binding (LBD) domains in complex with Ramp2 DNA

Sample:	Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 74 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Oct 7

R_g^Guinier	3.6	nm
D_max	12.2	nm

SASDAY4 – fH1015

Factor H CCP modules 10 to 15 experimental SAS data

Sample:	Factor H CCP modules 10 to 15 monomer, 41 kDa Homo sapiens protein
Buffer:	50 mM Potassium Phosphate, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 Dec 2

The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. J Mol Biol 395(1):105-22 (2010)
Schmidt CQ, Herbert AP, Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN

R_g^Guinier	3.0	nm
D_max	10.4	nm
Volume^Porod	68	nm³

SASDMY4 – Retinoic acid receptor RXR-alpha DNA binding domain (DBD) in complex with Ramp2

Sample:	Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 20 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Oct 7

R_g^Guinier	1.9	nm
D_max	5.8	nm

SASDAZ4 – fH1114

Factor H CCP modules 11 to 14 experimental SAS data

Sample:	Factor H CCP modules 11 to 14 monomer, 28 kDa Homo sapiens protein
Buffer:	50 mM Potassium Phosphate, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 Dec 2

R_g^Guinier	3.0	nm
D_max	10.5	nm
Volume^Porod	38	nm³

SASDMZ4 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml

Estrogen-related receptor gammaInverse repeat IR3 DNA experimental SAS data

Sample:	Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Mar 3

Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules Biochemistry 52(39):6844-6855 (2013)
Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D

R_g^Guinier	4.7	nm
D_max	14.6	nm

SASDA25 – fh1213

Factor H CCP modules 12 to 13 experimental SAS data

Sample:	Factor H CCP modules 12 to 13 monomer, 14 kDa Homo sapiens protein
Buffer:	50 mM Potassium Phosphate, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 Dec 2

R_g^Guinier	2.0	nm
D_max	7.1	nm
Volume^Porod	20	nm³

SASDM25 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.9 mg/ml

Sample:	Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Mar 3

R_g^Guinier	4.3	nm
D_max	13.2	nm

SASDB35 – Staphylococcus aureus thiaminase II

Thiaminase type II enzyme experimental SAS data

Sample:	Thiaminase type II enzyme tetramer, 107 kDa Staphylococcus aureus protein
Buffer:	100 mM Tris-HCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 May 11

Staphylococcus aureus thiaminase II: oligomerization warrants proteolytic protection against serine proteases. Acta Crystallogr D Biol Crystallogr 69(Pt 12):2320-9 (2013)
Begum A, Drebes J, Kikhney A, Müller IB, Perbandt M, Svergun D, Wrenger C, Betzel C

R_g^Guinier	3.4	nm
D_max	11.0	nm
Volume^Porod	168	nm³

SASDM35 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.0 mg/ml

Sample:	Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Mar 3

R_g^Guinier	4.1	nm
D_max	12.8	nm

SASDA45 – PpoA wild type enzyme

Psi-producing oxygenase A experimental SAS data

Sample:	Psi-producing oxygenase A trimer, 362 kDa Aspergillus nidulans protein
Buffer:	20 Mm HEPES, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Jun 24

A structural model of PpoA derived from SAXS-analysis-implications for substrate conversion. Biochim Biophys Acta 1831(9):1449-57 (2013)
Koch C, Tria G, Fielding AJ, Brodhun F, Valerius O, Feussner K, Braus GH, Svergun DI, Bennati M, Feussner I

R_g^Guinier	5.4	nm
D_max	16.5	nm
Volume^Porod	540	nm³

SASDA55 – Nucleoplasmin

Sample:	Nucleoplasmin pentamer, 110 kDa Escherichia coli protein
Buffer:	20 mM Pipes buffer 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Dec 3

A mechanism for histone chaperoning activity of nucleoplasmin: thermodynamic and structural models. J Mol Biol 393(2):448-63 (2009)
Taneva SG, Bañuelos S, Falces J, Arregi I, Muga A, Konarev PV, Svergun DI, Velázquez-Campoy A, Urbaneja MA

R_g^Guinier	4.0	nm
D_max	12.6	nm
Volume^Porod	210	nm³

SASDA65 – Nucleoplasmin-H5 complex

Sample:	NP-H5 pentamer, 200 kDa Escherichia coli protein
Buffer:	20 mM Pipes buffer 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Dec 3

R_g^Guinier	5.2	nm
D_max	16.7	nm
Volume^Porod	410	nm³

SASDA75 – Nucleoplasmin-H2AH2B complex

Sample:	NP-H2AH2B pentamer, 250 kDa Escherichia coli protein
Buffer:	20 mM Pipes buffer 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2014 Dec 3

R_g^Guinier	4.7	nm
D_max	14.5	nm
Volume^Porod	470	nm³

SASDA85 – CHD4 (PP-CC-AH-D)

Human Chromatin Remodeler CHD4 (363-1353) experimental SAS data

Sample:	Human Chromatin Remodeler CHD4 (363-1353) monomer, 117 kDa Homo sapiens protein
Buffer:	50 mM HEPES 5% Glycerol 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Nov 13

The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
Watson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, La...

R_g^Guinier	4.9	nm
D_max	17.4	nm

SASDA95 – CHD4 (CC-AH-D)

Human Chromatin Remodeler CHD4 (494-1353) experimental SAS data

Sample:	Human Chromatin Remodeler CHD4 (494-1353) monomer, 101 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Nov 13

R_g^Guinier	4.0	nm
D_max	14.5	nm

SASDAA5 – CHD4 (AH)

Human Chromatin Remodeler CHD4 (685-1233) experimental SAS data

Sample:	Human Chromatin Remodeler CHD4 (685-1233) monomer, 63 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Nov 13

R_g^Guinier	4.0	nm
D_max	14.5	nm

SASDAB5 – CHD4 (PP-CC)

Human Chromatin Remodeler CHD4 (363-682) experimental SAS data

Sample:	Human Chromatin Remodeler CHD4 (363-682) monomer, 38 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Nov 13

R_g^Guinier	3.0	nm
D_max	10.5	nm

SASDAC5 – ImportinA_ImportinB

ImportinA_ImportinB experimental SAS data

Sample:	ImportinA_ImportinB monomer, 160 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Oct 29

Recognition of nucleoplasmin by its nuclear transport receptor importin α/β: insights into a complete import complex. Biochemistry 49(45):9756-69 (2010)
Falces J, Arregi I, Konarev PV, Urbaneja MA, Svergun DI, Taneva SG, Bañuelos S

R_g^Guinier	5.7	nm
D_max	19.0	nm
Volume^Porod	390	nm³

SASDAD5 – Nucleoplasmin_ImpA_ImpB

Nucleoplasmin_importinA_importinB experimental SAS data

Sample:	Nucleoplasmin_importinA_importinB , 900 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 May 23

R_g^Guinier	8.6	nm
D_max	28.0	nm
Volume^Porod	1800	nm³

SASDAE5 – CYNEX4

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) experimental SAS data

Sample:	CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) monomer, 93 kDa Homo sapiens protein
Buffer:	50 mM HEPES 150 mM NaCl 1 mM EGTA, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Sep 11

Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI

R_g^Guinier	3.7	nm
D_max	12.8	nm
Volume^Porod	135	nm³

SASDAF5 – CYNEX4-T266D

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant experimental SAS data

Sample:	CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant monomer, 93 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Sep 11

Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI

R_g^Guinier	4.1	nm
D_max	14.4	nm
Volume^Porod	146	nm³

SASDAG5 – RNA shaperone Hfq

Sample:	RNA chaperone Hfq hexamer, 67 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 May 2

Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq. Nucleic Acids Res 39(11):4900-15 (2011)
Beich-Frandsen M, Vecerek B, Konarev PV, Sjöblom B, Kloiber K, Hämmerle H, Rajkowitsch L, Miles AJ, Kontaxis G, Wallace BA, Svergun DI, Konrat R, Bläsi U, Djinovic-Carugo K

R_g^Guinier	3.2	nm
D_max	11.2	nm
Volume^Porod	110	nm³

SASDLG5 – Human nerve growth factor

Beta-nerve growth factor experimental SAS data

Sample:	Beta-nerve growth factor dimer, 24 kDa Homo sapiens protein
Buffer:	50 mM Na-phosphate, 1 mM EDTA, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Jul 2

The conundrum of the high-affinity NGF binding site formation unveiled? Biophys J 108(3):687-97 (2015)
Covaceuszach S, Konarev PV, Cassetta A, Paoletti F, Svergun DI, Lamba D, Cattaneo A

R_g^Guinier

2.5

SASDAH5 – Complex of Hfq with DsrA

RNA chaperone HfqRNA DsrA experimental SAS data

Sample:	RNA chaperone Hfq hexamer, 67 kDa Escherichia coli protein RNA DsrA monomer, 12 kDa RNA
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Nov 16

Structural flexibility of RNA as molecular basis for Hfq chaperone function. Nucleic Acids Res 40(16):8072-84 (2012)
Ribeiro Ede A Jr, Beich-Frandsen M, Konarev PV, Shang W, Vecerek B, Kontaxis G, Hämmerle H, Peterlik H, Svergun DI, Bläsi U, Djinović-Carugo K

R_g^Guinier	4.3	nm
D_max	14.5	nm
Volume^Porod	210	nm³

SASDLH5 – Aggregation state of Ataxin-3 protein

Sample:	Ataxin-3 monomer, 41 kDa Homo sapiens protein
Buffer:	phosphate buffered saline (PBS), pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Dec 3

Interactions of ataxin-3 with its molecular partners in the protein machinery that sorts protein aggregates to the aggresome. Int J Biochem Cell Biol 51:58-64 (2014)
Bonanomi M, Mazzucchelli S, D'Urzo A, Nardini M, Konarev PV, Invernizzi G, Svergun DI, Vanoni M, Regonesi ME, Tortora P

R_g^Guinier	6.9	nm
D_max	25.0	nm
Volume^Porod	425	nm³

SASDAJ5 – Annexin-A4

Sample:	Annexin-A4 monomer, 36 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Sep 11

Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI

R_g^Guinier	2.4	nm
D_max	7.6	nm
Volume^Porod	54	nm³

SASDLJ5 – Aggregation state of tubulin protein

Tubulin alpha-1A chain experimental SAS data

Sample:	Tubulin alpha-1A chain monomer, 50 kDa Homo sapiens protein
Buffer:	phosphate buffered saline (PBS), pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Dec 3

R_g^Guinier	7.0	nm
D_max	25.0	nm
Volume^Porod	340	nm³

SASDAK5 – Myomesin-1 My12-My13

Sample:	Myomesin-1 dimer, 46 kDa Homo sapiens protein
Buffer:	25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 May 7

Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin. EMBO J 27(1):253-64 (2008)
Pinotsis N, Lange S, Perriard JC, Svergun DI, Wilmanns M

R_g^Guinier	4.0	nm
D_max	14.5	nm
Volume^Porod	56	nm³

SASDLK5 – Aggregation state of ataxin-3-tubulin complex

Ataxin-3Tubulin alpha-1A chain experimental SAS data

Sample:	Ataxin-3 monomer, 41 kDa Homo sapiens protein Tubulin alpha-1A chain monomer, 50 kDa Homo sapiens protein
Buffer:	phosphate buffered saline (PBS), pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Dec 3

R_g^Guinier	8.4	nm
D_max	30.0	nm
Volume^Porod	900	nm³

SASDAL5 – Clostridium difficile bacteriophage 27 endolysin dimer, CD27L

Clostridium difficile bacteriophage 27 endolysin experimental SAS data

Sample:	Clostridium difficile bacteriophage 27 endolysin dimer, 64 kDa Clostridioides difficile protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Mar 17

The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor. PLoS Pathog 10(7):e1004228 (2014)
Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R

R_g^Guinier	3.3	nm
D_max	10.6	nm
Volume^Porod	72	nm³

SASDLL5 – frataxin homolog, Yfh1, at 0 °C

Frataxin homolog, mitochondrial experimental SAS data

frataxin homolog, Yfh1, at 0 °C Rg histogram

Sample:	Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM HEPES, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 Nov 28

The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1. J Mol Biol 417(5):413-24 (2012)
Adrover M, Martorell G, Martin SR, Urosev D, Konarev PV, Svergun DI, Daura X, Temussi P, Pastore A

R_g^Guinier

2.2

SASDLM5 – frataxin homolog, Yfh1, at 20 °C

frataxin homolog, Yfh1, at 20 °C Rg histogram

Sample:	Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM HEPES, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 Nov 28

R_g^Guinier

2.1

SASDAN5 – Cross-linked complex CytC_Adr

Cytochrome CAdrenodoxin experimental SAS data

Sample:	Cytochrome C monomer, 11 kDa Escherichia coli protein Adrenodoxin monomer, 11 kDa Escherichia coli protein
Buffer:	20 mM HEPES 2 mM DTT, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2006 Jun 15

Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy. J Am Chem Soc 130(20):6395-403 (2008)
Xu X, Reinle W, Hannemann F, Konarev PV, Svergun DI, Bernhardt R, Ubbink M

R_g^Guinier	2.1	nm
D_max	8.5	nm
Volume^Porod	42	nm³

SASDLN5 – frataxin homolog, Yfh1, at 50 °C

frataxin homolog, Yfh1, at 50 °C Rg histogram

Sample:	Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM HEPES, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2008 Nov 28

R_g^Guinier

2.5

SASDAP5 – Native complex CytC_Adr

Cytochrome C dimerAdrenodoxin dimer experimental SAS data

Sample:	Cytochrome C dimer dimer, 22 kDa Escherichia coli protein Adrenodoxin dimer dimer, 22 kDa Escherichia coli protein
Buffer:	20 mM HEPES 2 mM DTT, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2006 Jun 15

R_g^Guinier	2.9	nm
D_max	9.5	nm
Volume^Porod	64	nm³

SASDAQ5 – Lumazine Synthase

Sample:	Lumazine Synthase , 960 kDa Bacillus subtilis protein
Buffer:	Borate buffer, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2004 Nov 25

Multiple assembly states of lumazine synthase: a model relating catalytic function and molecular assembly. J Mol Biol 362(4):753-70 (2006)
Zhang X, Konarev PV, Petoukhov MV, Svergun DI, Xing L, Cheng RH, Haase I, Fischer M, Bacher A, Ladenstein R, Meining W

R_g^Guinier	6.2	nm
D_max	15.5	nm
Volume^Porod	1450	nm³

SASDLQ5 – Tricorn protease oligomers

Sample:	Tricorn protease hexamer, 730 kDa Thermoplasma acidophilum (strain … protein
Buffer:	20mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2003 Jan 14

X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum. J Biol Chem 280(39):33387-96 (2005)
Goettig P, Brandstetter H, Groll M, Göhring W, Konarev PV, Svergun DI, Huber R, Kim JS

R_g^Guinier

5.9

SASDAR5 – ProNGF

Sample:	ProNGF dimer, 45 kDa Mouse submandibulary glands protein
Buffer:	50 mM Naphosphat 0.5 M Ammonium Sulfate(NH4)2SO4, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Oct 25

Intrinsic structural disorder of mouse proNGF. Proteins 75(4):990-1009 (2009)
Paoletti F, Covaceuszach S, Konarev PV, Gonfloni S, Malerba F, Schwarz E, Svergun DI, Cattaneo A, Lamba D

R_g^Guinier	2.6	nm
D_max	8.0	nm
Volume^Porod	92	nm³

SASDLR5 – Multidomain Regulatory Protein Rv1364c (monomer)

Multidomain regulatory protein Rv1364c experimental SAS data

Sample:	Multidomain regulatory protein Rv1364c monomer, 70 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	25 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Feb 14

Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis. Structure 19(1):56-69 (2011)
King-Scott J, Konarev PV, Panjikar S, Jordanova R, Svergun DI, Tucker PA

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	130	nm³

SASDAS5 – ad11 Fab

Sample:	aD11 Fab dimer, 50 kDa Mus musculus protein
Buffer:	50 mM Na-phosphate 1mM ethylenediaminetetraacetic aci, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Oct 20

Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody. J Mol Biol 381(4):881-96 (2008)
Covaceuszach S, Cassetta A, Konarev PV, Gonfloni S, Rudolph R, Svergun DI, Lamba D, Cattaneo A

R_g^Guinier	2.6	nm
D_max	8.0	nm
Volume^Porod	95	nm³

SASDLS5 – Multidomain Regulatory Protein Rv1364c (dimer)

Sample:	Multidomain regulatory protein Rv1364c dimer, 139 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	25 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Feb 14

R_g^Guinier	4.5	nm
D_max	18.0	nm
Volume^Porod	289	nm³

SASDAT5 – NGF

Sample:	NGF dimer, 50 kDa Mus musculus protein
Buffer:	10 mM Na-phosphate 150 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Oct 20

R_g^Guinier	3.1	nm
D_max	12.0	nm
Volume^Porod	102	nm³

SASDLT5 – Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA

Modification methylase SsoII12-bp DNA experimental SAS data

Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA Rg histogram

Sample:	Modification methylase SsoII monomer, 43 kDa Shigella sonnei protein 12-bp DNA monomer, 8 kDa DNA
Buffer:	50 mM Na-phosphate buffer, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Mar 13

Flexibility of the linker between the domains of DNA methyltransferase SsoII revealed by small-angle X-ray scattering: implications for transcription regulation in SsoII restriction-modification syste... PLoS One 9(4):e93453 (2014)
Konarev PV, Kachalova GS, Ryazanova AY, Kubareva EA, Karyagina AS, Bartunik HD, Svergun DI

R_g^Guinier	2.8	nm
D_max	11.0	nm
Volume^Porod	85	nm³

SASDAU5 – ad11 Fab + NGF

Sample:	aD11 Fab dimer, 50 kDa Mus musculus protein NGF dimer, 50 kDa Mus musculus protein
Buffer:	30 mM Na-phosphate 75 mM NaCl, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Dec 20

R_g^Guinier	4.3	nm
D_max	16.0	nm
Volume^Porod	255	nm³

SASDLU5 – Complex formed between the replication origin-binding protein UL9 (C-terminal domains) with 15-bp DNA

Replication origin-binding protein15-bp DNA experimental SAS data

Sample:	Replication origin-binding protein dimer, 81 kDa Human herpesvirus 1 … protein 15-bp DNA monomer, 9 kDa DNA
Buffer:	20 mM Tris-HCl, 50 mM NaCl, 5 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2006 May 27

Structural and biophysical characterization of the proteins interacting with the herpes simplex virus 1 origin of replication. J Biol Chem 284(24):16343-16353 (2009)
Manolaridis I, Mumtsidu E, Konarev P, Makhov AM, Fullerton SW, Sinz A, Kalkhof S, McGeehan JE, Cary PD, Griffith JD, Svergun D, Kneale GG, Tucker PA

R_g^Guinier	3.6	nm
D_max	15.0	nm

SASDAV5 – apo XMRV RT

Sample:	apo XMRV RT monomer, 75 kDa Escherichia coli protein
Buffer:	10 mM HEPES 100 mM KCl 5% Glycerol, pH: 6.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Dec 8

Structural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid. Nucleic Acids Res 41(6):3874-87 (2013)
Nowak E, Potrzebowski W, Konarev PV, Rausch JW, Bona MK, Svergun DI, Bujnicki JM, Le Grice SF, Nowotny M

R_g^Guinier	4.0	nm
D_max	13.5	nm
Volume^Porod	160	nm³

SASDLV5 – Myosin Filament-Linking Protein Myomesin-1 (domains My9-My13, dimer)

Sample:	Myomesin-1 dimer, 121 kDa Homo sapiens protein
Buffer:	25 mMTris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 May 10

Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. PLoS Biol 10(2):e1001261 (2012)
Pinotsis N, Chatziefthimiou SD, Berkemeier F, Beuron F, Mavridis IM, Konarev PV, Svergun DI, Morris E, Rief M, Wilmanns M

R_g^Guinier	9.6	nm
D_max	37.0	nm

SASDAW5 – XMRV RT + DNA/RNA hybrid

apo XMRV RTRNA_DNA hybrid substrate experimental SAS data

Sample:	apo XMRV RT monomer, 75 kDa Escherichia coli protein RNA_DNA hybrid substrate monomer, 15 kDa
Buffer:	10 mM HEPES 100 mM KCl 5% Glycerol, pH: 6.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Dec 8

R_g^Guinier	3.5	nm
D_max	11.5	nm
Volume^Porod	155	nm³

SASDLW5 – Cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens

Sample:	Cytochrome c-552 hexamer, 356 kDa Thioalkalivibrio nitratireducens (strain … protein
Buffer:	Tris-borate buffer, pH: 8.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 May 8

Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens. Biochemistry (Mosc) 73(2):164-70 (2008)
Tikhonova TV, Slutskaya ES, Filimonenkov AA, Boyko KM, Kleimenov SY, Konarev PV, Polyakov KM, Svergun DI, Trofimov AA, Khomenkov VG, Zvyagilskaya RA, Popov VO

R_g^Guinier

4.8

SASDAX5 – Endophilin-CoA complex

Endophilin-A1 BAR domainarachidonyl-CoA experimental SAS data

Sample:	Endophilin-A1 BAR domain dimer, 58 kDa Mus musculus protein arachidonyl-CoA , 60 kDa
Buffer:	50 mM TRIS-HCL 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2006 Nov 27

Endophilin-A1 BAR domain interaction with arachidonyl CoA. Front Mol Biosci 1:20 (2014)
Petoukhov MV, Weissenhorn W, Svergun DI

R_g^Guinier	5.9	nm
D_max	19.0	nm
Volume^Porod	480	nm³

SASDLX5 – Wild-type human mitochondrial deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial experimental SAS data

Sample:	Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial trimer, 80 kDa Homo sapiens protein
Buffer:	20 mM citrate buffer, 1 mM DTT, 0.1 mM PMSF and 1 mM MgCl2, pH: 5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Feb 1

Molecular shape and prominent role of β-strand swapping in organization of dUTPase oligomers FEBS Letters 583(5):865-871 (2009)
Takács E, Barabás O, Petoukhov M, Svergun D, Vértessy B

R_g^Guinier	2.6	nm
D_max	7.0	nm
Volume^Porod	101	nm³

SASDAY5 – Free endophilin

Endophilin-A1 BAR domain experimental SAS data

Sample:	Endophilin-A1 BAR domain dimer, 58 kDa Mus musculus protein
Buffer:	50 mM TRIS-HCL 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2004 Feb 13

Endophilin-A1 BAR domain interaction with arachidonyl CoA. Front Mol Biosci 1:20 (2014)
Petoukhov MV, Weissenhorn W, Svergun DI

R_g^Guinier	3.3	nm
D_max	13.5	nm
Volume^Porod	90	nm³

SASDLY5 – Monomer-dimer equilibrium of glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data

Sample:	Glutamate--tRNA ligase dimer, 108 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	35 mM HEPES⁄NaOH, pH: 6.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Feb 19

Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl-tRNA synthetase and determination of its oligomeric structure in solution FEBS Journal 276(5):1398-1417 (2009)
Paravisi S, Fumagalli G, Riva M, Morandi P, Morosi R, Konarev P, Petoukhov M, Bernier S, Chênevert R, Svergun D, Curti B, Vanoni M

R_g^Guinier	3.5	nm
D_max	11.0	nm
Volume^Porod	123	nm³

SASDLZ5 – Hepatocyte growth factor/Hepatocyte growth factor receptor (NK1-Met567) complex

Hepatocyte growth factorHepatocyte growth factor receptor experimental SAS data

Sample:	Hepatocyte growth factor dimer, 39 kDa Homo sapiens protein Hepatocyte growth factor receptor dimer, 122 kDa Homo sapiens protein
Buffer:	Phosphate-buffered saline containing EDTA, surfactant P20 and 5 M NaCl, pH: 6
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Jun 26

Engineering the NK1 Fragment of Hepatocyte Growth Factor/Scatter Factor as a MET Receptor Antagonist Journal of Molecular Biology 377(3):616-622 (2008)
Youles M, Holmes O, Petoukhov M, Nessen M, Stivala S, Svergun D, Gherardi E

R_g^Guinier	5.3	nm
D_max	20.0	nm
Volume^Porod	290	nm³

SASDA56 – BAZ2B in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminal experimental SAS data

Sample:	Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa Homo sapiens protein
Buffer:	20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2012 Sep 24

Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC. Structure 23(1):80-92 (2015)
Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A

R_g^Guinier	4.0	nm
D_max	14.5	nm
Volume^Porod	43	nm³

SASDC56 – Blue light receptor YtvA, dark/inactive state

pfyP - Blue light photoreceptor experimental SAS data

Sample:	pfyP - Blue light photoreceptor dimer, 58 kDa Bacillus subtilis protein
Buffer:	PBS + 5 mM DTT, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Oct 24

The switch that does not flip: the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state as revealed by a combined AUC and SAXS stu... J Mol Biol 403(1):78-87 (2010)
Jurk M, Dorn M, Kikhney A, Svergun D, Gärtner W, Schmieder P

R_g^Guinier	3.2	nm
D_max	10.1	nm
Volume^Porod	75	nm³

SASDA66 – BAZ2B-18mer complex in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminalH3Kac9Kac14 experimental SAS data

Bromodomain adjacent to zinc finger domain protein 2B, C-terminal H3Kac9Kac14 Kratky plot

Sample:	Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa Homo sapiens protein H3Kac9Kac14 monomer, 5 kDa Homo sapiens protein
Buffer:	20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2012 Sep 24

R_g^Guinier	4.2	nm
D_max	16.0	nm
Volume^Porod	52	nm³

SASDC66 – Blue light receptor YtvA, lit/active state

Sample:	pfyP - Blue light photoreceptor dimer, 58 kDa Bacillus subtilis protein
Buffer:	PBS + 5 mM DTT, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Oct 24

R_g^Guinier	3.2	nm
D_max	10.1	nm
Volume^Porod	74	nm³

SASDAH6 – WbdD(1-459)

bifunctional kinase- methyltransferase WbdD experimental SAS data

Sample:	bifunctional kinase- methyltransferase WbdD monomer, 59 kDa Escherichia coli protein
Buffer:	20 mM BisTris 50 mM NaCl 5 mM DTT, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Sep 23

A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide. Nat Struct Mol Biol 22(1):50-56 (2015)
Hagelueken G, Clarke BR, Huang H, Tuukkanen A, Danciu I, Svergun DI, Hussain R, Liu H, Whitfield C, Naismith JH

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	90	nm³

SASDAJ6 – WbdD(1-556)

Sample:	bifunctional kinase- methyltransferase WbdD trimer, 190 kDa protein
Buffer:	20 mM BisTris 50 mM NaCl 5 mM DTT, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Dec 2

R_g^Guinier	5.2	nm
D_max	17.0	nm
Volume^Porod	380	nm³

SASDAK6 – Glucose Isomerase

Sample:	Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer:	PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2012 Apr 24

Standard proteins
Erica Valentini

R_g^Guinier	3.4	nm
D_max	9.7	nm
Volume^Porod	293	nm³

SASDAR6 – human CSF-1:CSF-1R extracellular signalling complex

Macrophage colony-stimulating factor 1Macrophage colony-stimulating factor 1 receptor experimental SAS data

Sample:	Macrophage colony-stimulating factor 1 dimer, 35 kDa Homo sapiens protein Macrophage colony-stimulating factor 1 receptor dimer, 107 kDa Homo sapiens protein
Buffer:	50 mM NaH2PO4, 100 m, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Mar 13

Structure and Assembly Mechanism of the Signaling Complex Mediated by Human CSF-1. Structure 23(9):1621-1631 (2015)
Felix J, De Munck S, Verstraete K, Meuris L, Callewaert N, Elegheert J, Savvides SN

R_g^Guinier	5.7	nm
D_max	17.9	nm
Volume^Porod	299	nm³

SASDET6 – Polyglutamine-binding protein 1 p.Lys192Serfs7 (PQBP-1 XLID mutant K192Sfs7)

Polyglutamine-binding protein 1 p.Lys192Serfs*7 experimental SAS data

Polyglutamine-binding protein 1 p.Lys192Serfs*7 (PQBP-1 XLID mutant K192Sfs*7) Rg histogram

Sample:	Polyglutamine-binding protein 1 p.Lys192Serfs7 dimer, 47 kDa Homo sapiens* protein
Buffer:	Phosphate-buffered saline, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2013 Feb 15

Frameshift PQBP-1 mutants K192Sfs*7 and R153Sfs*41 implicated in X-linked intellectual disability form stable dimers. J Struct Biol (2019)
Rahman SK, Okazawa H, Chen YW

R_g^Guinier	3.5	nm
D_max	13.0	nm
Volume^Porod	114	nm³

SASDEU6 – Polyglutamine-binding protein 1 p.Arg153Serfs41 (PQBP-1 XLID mutant R153Sfs41)

Polyglutamine-binding protein 1 p.Arg153Serfs*41 experimental SAS data

Polyglutamine-binding protein 1 p.Arg153Serfs*41 (PQBP-1 XLID mutant R153Sfs*41) Rg histogram

Sample:	Polyglutamine-binding protein 1 p.Arg153Serfs41 dimer, 44 kDa Homo sapiens* protein
Buffer:	Phosphate-buffered saline, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2013 Feb 15

Frameshift PQBP-1 mutants K192Sfs*7 and R153Sfs*41 implicated in X-linked intellectual disability form stable dimers. J Struct Biol (2019)
Rahman SK, Okazawa H, Chen YW

R_g^Guinier	3.6	nm
D_max	13.0	nm
Volume^Porod	100	nm³

SASDAV6 – Cysteine desulfurase IscS dimer

Cysteine desulfurase IscS experimental SAS data

Sample:	Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Nov 5

Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat Commun 1:95 (2010)
Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A

R_g^Guinier	3.2	nm
D_max	10.9	nm

SASDAW6 – Iron-sulfur cluster assembly scaffold IscU monomer

Iron-sulfur cluster assembly scaffold protein IscU experimental SAS data

Sample:	Iron-sulfur cluster assembly scaffold protein IscU monomer, 14 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	1.9	nm
D_max	6.4	nm

SASDAX6 – Protein CyaY monomer

Sample:	Protein CyaY monomer, 12 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	1.5	nm
D_max	5.0	nm

SASDAY6 – IcsS-dimer and IscU-dimer complex

Cysteine desulfurase IscSIron-sulfur cluster assembly scaffold protein IscU experimental SAS data

Sample:	Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Iron-sulfur cluster assembly scaffold protein IscU dimer, 29 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	3.6	nm
D_max	12.1	nm

SASDAZ6 – CyaY-dimer and IscS-dimer complex

Cysteine desulfurase IscSProtein CyaY experimental SAS data

Sample:	Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Protein CyaY dimer, 24 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl, 50 mM NaCl, 10 mM β-mercaptoethanol and ferrous ammonium sulphate, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	3.1	nm
D_max	10.9	nm

SASDLZ6 – Hexamer - monomer equilibrium of Glutamate Synthase

Glutamate synthase [NADPH] large chainGlutamate synthase [NADPH] small chain experimental SAS data

Sample:	Glutamate synthase [NADPH] large chain hexamer, 968 kDa Azospirillum brasilense protein Glutamate synthase [NADPH] small chain hexamer, 296 kDa Azospirillum brasilense protein
Buffer:	25 mM Hepes/KOH, 1 mM EDTA, 1 mM dithiothreitol, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Feb 19

The Subnanometer Resolution Structure of the Glutamate Synthase 1.2-MDa Hexamer by Cryoelectron Microscopy and Its Oligomerization Behavior in Solution Journal of Biological Chemistry 283(13):8237-8249 (2008)
Cottevieille M, Larquet E, Jonic S, Petoukhov M, Caprini G, Paravisi S, Svergun D, Vanoni M, Boisset N

R_g^Guinier	7.7	nm
D_max	22.4	nm

SASDA27 – IcsS, IscU and CyaY dimeric complex

Cysteine desulfurase IscSIron-sulfur cluster assembly scaffold protein IscUProtein CyaY experimental SAS data

Sample:	Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Iron-sulfur cluster assembly scaffold protein IscU dimer, 29 kDa Escherichia coli protein Protein CyaY dimer, 24 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	3.5	nm
D_max	11.9	nm

SASDL27 – N-Oct-3 POU transcription factor domain in complex with rat CRH DNA

POU domain, class 3, transcription factor 2Rat CRH DNA experimental SAS data

Sample:	POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein Rat CRH DNA monomer, 15 kDa Rattus norvegicus DNA
Buffer:	50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2003 Oct 9

Fine-tuning of intrinsic N-Oct-3 POU domain allostery by regulatory DNA targets Nucleic Acids Research 35(13):4420-4432 (2007)
Alazard R, Mourey L, Ebel C, Konarev P, Petoukhov M, Svergun D, Erard M

R_g^Guinier	2.9	nm
D_max	11.0	nm
Volume^Porod	41	nm³

SASDL37 – N-Oct-3 POU transcription factor domain in complex with human DR-alpha DNA

POU domain, class 3, transcription factor 2Human DR-alpha DNA experimental SAS data

Sample:	POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein Human DR-alpha DNA monomer, 15 kDa Homo sapiens DNA
Buffer:	50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2004 May 26

R_g^Guinier	2.9	nm
D_max	11.0	nm
Volume^Porod	44	nm³

SASDL47 – Oligomeric composition of AXH Domain of Ataxin-1 (wild type and A567G, I580A mutants)

Sample:	Ataxin-1 monomer, 14 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Oct 21

Self-assembly and conformational heterogeneity of the AXH domain of ataxin-1: an unusual example of a chameleon fold. Biophys J 104(6):1304-13 (2013)
de Chiara C, Rees M, Menon RP, Pauwels K, Lawrence C, Konarev PV, Svergun DI, Martin SR, Chen YW, Pastore A

R_g^Guinier

5.5

SASDM77 – Full-length polypyrimidine tract binding protein (PTB)

Polypyrimidine tract-binding protein 2 experimental SAS data

Sample:	Polypyrimidine tract-binding protein 2 monomer, 57 kDa Homo sapiens protein
Buffer:	25 mM Tris, 250 mM NaCl, 2 mM DTT, pH: 7.2
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2004 Feb 11

Structure and RNA interactions of the N-terminal RRM domains of PTB. Structure 12(9):1631-43 (2004)
Simpson PJ, Monie TP, Szendröi A, Davydova N, Tyzack JK, Conte MR, Read CM, Cary PD, Svergun DI, Konarev PV, Curry S, Matthews S

R_g^Guinier	4.0	nm
D_max	14.0	nm
Volume^Porod	117	nm³

SASDM87 – Mouse ProNGF - pro-form of the NGF (nerve growth factor)

Mouse ProNGF - pro-form of the NGF (nerve growth factor) experimental SAS data

Sample:	Mouse ProNGF - pro-form of the NGF (nerve growth factor) dimer, 100 kDa Mus musculus protein
Buffer:	50 mM Na-phosphate, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Jul 18

Structural and functional properties of mouse proNGF. Biochem Soc Trans 34(Pt 4):605-6 (2006)
Paoletti F, Konarev PV, Covaceuszach S, Schwarz E, Cattaneo A, Lamba D, Svergun DI

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	97	nm³

SASDC97 – Ethylene Receptor 1 (DHp + CA + RD domains)

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains experimental SAS data

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains Kratky plot

Sample:	Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains dimer, 88 kDa Arabidopsis thaliana protein
Buffer:	20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP, pH: 8.8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Oct 11

Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
Mayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J

R_g^Guinier	4.0	nm
D_max	13.9	nm
Volume^Porod	144	nm³

SASDM97 – Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv)

Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv) experimental SAS data

Sample:	Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv) monomer, 29 kDa protein
Buffer:	phosphate buffered saline, pH: 7
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Oct 21

Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment. Arch Biochem Biophys 522(1):26-36 (2012)
Paoletti F, Malerba F, Konarev PV, Visintin M, Scardigli R, Fasulo L, Lamba D, Svergun DI, Cattaneo A

R_g^Guinier	2.7	nm
D_max	8.0	nm
Volume^Porod	39	nm³

SASDCA7 – Ethylene Receptor 1 (DHp + CA domains)

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains experimental SAS data

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains Kratky plot

Sample:	Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains dimer, 55 kDa Arabidopsis thaliana protein
Buffer:	20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP, pH: 8.8
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2010 Oct 11

R_g^Guinier	2.7	nm
D_max	8.7	nm
Volume^Porod	74	nm³

SASDMA7 – Prion protein aggregate in solution

Major prion protein experimental SAS data

Sample:	Major prion protein 24-mer, 664 kDa Homo sapiens protein
Buffer:	5 mM sodium acetate, pH: 5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Jun 2

Structural characterization of beta-sheeted oligomers formed on the pathway of oxidative prion protein aggregation in vitro. J Struct Biol 157(2):308-20 (2007)
Redecke L, von Bergen M, Clos J, Konarev PV, Svergun DI, Fittschen UE, Broekaert JA, Bruns O, Georgieva D, Mandelkow E, Genov N, Betzel C

R_g^Guinier	9.8	nm
D_max	32.0	nm
Volume^Porod	3320	nm³

SASDLB7 – Procollagen C-proteinase enhancer

Procollagen C-endopeptidase enhancer 1 experimental SAS data

Sample:	Procollagen C-endopeptidase enhancer 1 monomer, 46 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 500 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2002 Jul 9

Low Resolution Structure Determination Shows Procollagen C-Proteinase Enhancer to be an Elongated Multidomain Glycoprotein Journal of Biological Chemistry 278(9):7199-7205 (2003)
Bernocco S, Steiglitz B, Svergun D, Petoukhov M, Ruggiero F, Ricard-Blum S, Ebel C, Geourjon C, Deléage G, Font B, Eichenberger D, Greenspan D, Hulmes D

R_g^Guinier	4.1	nm
D_max	15.0	nm
Volume^Porod	117	nm³

SASDMB7 – Glucosamine-6-phosphate Synthase from Candida albicans

N-acetylglucosamine kinase 1 experimental SAS data

Sample:	N-acetylglucosamine kinase 1 tetramer, 219 kDa Candida albicans (strain … protein
Buffer:	0.2 M magnesium acetate, 0.1 M sodium cacodylate, pH: 6.5
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2005 Apr 28

The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans. J Mol Biol 372(3):672-88 (2007)
Raczynska J, Olchowy J, Konariev PV, Svergun DI, Milewski S, Rypniewski W

R_g^Guinier	5.1	nm
D_max	16.0	nm
Volume^Porod	421	nm³

SASDMC7 – Nuclear fragile X mental retardation-interacting protein 2 (FRMP)

Nuclear fragile X mental retardation-interacting protein 2 experimental SAS data

Sample:	Nuclear fragile X mental retardation-interacting protein 2 monomer, 59 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl pH 8.0, 5 mM TCEP, pH:
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2007 Oct 11

A study of the ultrastructure of fragile-X-related proteins. Biochem J 419(2):347-57 (2009)
Sjekloća L, Konarev PV, Eccleston J, Taylor IA, Svergun DI, Pastore A

R_g^Guinier	4.1	nm
D_max	15.0	nm
Volume^Porod	92	nm³

SASDAD7 – Structure of a complex between full length and truncated CTP1L endolysin

Sample:	Endolysin , 33 kDa Clostridium phage phiCTP1 protein
Buffer:	20 mM HEPES, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Mar 17

Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product. J Biol Chem 291(10):4882-93 (2016)
Dunne M, Leicht S, Krichel B, Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R

R_g^Guinier	3.7	nm
D_max	13.8	nm
Volume^Porod	95	nm³

SASDAE7 – Structure of a complex between full length and truncated CS74L endolysin

Sample:	Endolysin CS74L , 31 kDa Clostridium phage phi8074-B1 protein
Buffer:	20 mM HEPES, pH: 7.4
Experiment:	SAXS data collected at ...X33, DORIS III, DESY on 2011 Mar 17

R_g^Guinier	3.6	nm
D_max	14.0	nm
Volume^Porod	79	nm³