Small Angle Scattering Biological Data Bank SASBDB


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84 hits found for Toxin

SASDL62 – ...toxin CyaA - construct RTX-1 (amino acids 1132-1294 and 1562-1681 of CyaA)

hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) experimental SAS data

Sample:	hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) monomer, 30 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

...toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L

R_g^Guinier	2.4	nm
D_max	8.2	nm
Volume^Porod	41	nm³

SASDL72 – ...toxin CyaA - construct RTX-2 (amino acids 1132-1303 and 1562-1681 of CyaA)

hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) experimental SAS data

Sample:	hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) monomer, 31 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

...toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	37	nm³

SASDC23 – Colicin N WT

Sample:	Colicin N monomer, 43 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

...Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	74	nm³

SASDC33 – Colicin N delta 1-39

Colicin N delta 1-39 experimental SAS data

Sample:	Colicin N delta 1-39 monomer, 39 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

...Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	2.7	nm
D_max	9.0	nm
Volume^Porod	60	nm³

SASDD33 – Toxin/Antitoxin complex from M. tuberculosis

Mycobacterial cidal toxinMycobacterial cidal antitoxin experimental SAS data

Sample:	...toxin hexamer, 121 kDa Mycobacterium tuberculosis protein ...toxin hexamer, 76 kDa Mycobacterium tuberculosis protein
Buffer:	100 mM HEPES, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2

...Toxin Triggers Mycobacterium tuberculosis Cell Death. Mol Cell (2019)
Freire DM, Gutierrez C, Garza-Garcia A, Grabowska AD, Sala AJ, Ariyachaokun K, Panikova T, Beckham KSH, Colom A, Pogenberg V, Cianci M, Tuukkanen A, Boudehen YM, Peixoto A, Botella L, Svergun DI, Schn...

R_g^Guinier	4.1	nm
D_max	11.4	nm
Volume^Porod	262	nm³

SASDC43 – Colicin K145A mutant

Colicin N K145A mutant experimental SAS data

Sample:	Colicin N K145A mutant monomer, 43 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

...Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	3.2	nm
D_max	13.6	nm
Volume^Porod	68	nm³

SASDD43 – ...toxin (MbsT)

Mycobacterial cidal toxin experimental SAS data

Sample:	...toxin monomer, 20 kDa Mycobacterium tuberculosis H37Rv protein
Buffer:	30 mM Tris-HCl, 200 mM NaCl, 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 2

MbsTA
Diana Freire

R_g^Guinier	1.8	nm
D_max	5.6	nm
Volume^Porod	30	nm³

SASDC53 – Colicin N Translocation domain

Colicin N Translocation domain experimental SAS data

Colicin N Translocation domain Rg histogram

Sample:	Colicin N Translocation domain monomer, 10 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

...Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	2.8	nm
D_max	11.4	nm
Volume^Porod	22	nm³

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	...toxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	...toxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	...toxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDEN3 – Perivitellin PmPV2

P. maculata perivitellin 2 experimental SAS data

Sample:	P. maculata perivitellin 2 dimer, 188 kDa Pomacea maculata protein
Buffer:	20 mM Tris, pH: 7
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 Mar 26

...toxin in snails. J Struct Biol 211(2):107531 (2020)
Giglio ML, Ituarte S, Milesi V, Dreon MS, Brola TR, Caramelo J, Ip JCH, Maté S, Qiu JW, Otero LH, Heras H

R_g^Guinier	4.4	nm
D_max	14.3	nm
Volume^Porod	267	nm³

SASDPN3 – ...toxin:toxin complex

Antitoxin ParDToxin experimental SAS data

Sample:	...toxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 6

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	140	nm³

SASDJS3 – ...toxin CyaA RTX block II-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 57 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

...Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L

R_g^Guinier	4.0	nm
D_max	13.3	nm
Volume^Porod	94	nm³

SASDJT3 – ...toxin CyaA RTX block III-V

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

...Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L

R_g^Guinier	3.4	nm
D_max	12.1	nm
Volume^Porod	85	nm³

SASDJU3 – ...toxin CyaA RTX block IV-V

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

...Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L

R_g^Guinier	2.7	nm
D_max	8.6	nm
Volume^Porod	45	nm³

SASDJ64 – Calcium bound Calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

...Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.3	nm
Volume^Porod	27	nm³

SASDB74 – CyaA Block I-V

Adenylate cyclase toxin Block I-V experimental SAS data

Sample:	...toxin Block I-V monomer, 70 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
Bumba L, Masin J, Macek P, Wald T, Motlova L, Bibova I, Klimova N, Bednarova L, Veverka V, Kachala M, Svergun DI, Barinka C, Sebo P

R_g^Guinier	5.6	nm
D_max	17.2	nm
Volume^Porod	275	nm³

SASDJ74 – ...toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDB84 – CyaA Block V

Adenylate cyclase toxin Block V experimental SAS data

Sample:	...toxin Block V monomer, 16 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	1.8	nm
D_max	5.9	nm
Volume^Porod	24	nm³

SASDQN4 – ...toxin (CalpT10)

SAVED domain-containing proteinUncharacterized protein (putative MazF-like toxin) experimental SAS data

Sample:	SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein ...toxin) monomer, 9 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer:	20 mM Tris, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14

Antiviral signalling by a cyclic nucleotide activated CRISPR protease. Nature 614(7946):168-174 (2023)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G

R_g^Guinier	3.4	nm
D_max	11.7	nm
Volume^Porod	93	nm³

SASDXZ4 – Hemolysin A Type 1 Secretion System (Hly T1SS)

Alpha-hemolysin translocation ATP-binding protein HlyBHlyA (GFP-Hemolysin with Flag-Tag insertion)Membrane fusion protein (MFP) family protein (R14C; L229V)Outer membrane protein TolC experimental SAS data

Sample:	Alpha-hemolysin translocation ATP-binding protein HlyB hexamer, 480 kDa Escherichia coli (strain … protein HlyA (GFP-Hemolysin with Flag-Tag insertion) monomer, 144 kDa Escherichia coli protein Membrane fusion protein (MFP) family protein (R14C; L229V) hexamer, 327 kDa Escherichia coli (strain … protein Outer membrane protein TolC trimer, 161 kDa Escherichia coli (strain … protein
Buffer:	50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.0063% glyco-diosgenin (GDN), pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jun 29

...Toxin Recruitment of the HlyA E. coli T1SS Journal of Molecular Biology :169485 (2025)
Gentile R, Schott-Verdugo S, Khosa S, Günes C, Bonus M, Reiners J, Smits S, Schmitt L, Gohlke H

R_g^Guinier	11.4	nm
D_max	46.2	nm
Volume^Porod	2837	nm³

SASDR25 – ...toxin transcriptional activator ToxR

Cholera toxin transcriptional activator experimental SAS data

Sample:	...toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6

Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T

R_g^Guinier	1.6	nm
D_max	6.0	nm
Volume^Porod	16	nm³

SASDD45 – ...toxin-antitoxin system HicB family antitoxin - HicB protein

Type II toxin-antitoxin system HicB family antitoxin experimental SAS data

Sample:	...toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Buffer:	25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2016 Jul 28

...toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP

R_g^Guinier	3.0	nm
D_max	10.5	nm
Volume^Porod	112	nm³

SASDR45 – ...toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxS experimental SAS data

Sample:	...toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6

R_g^Guinier	2.1	nm
D_max	6.6	nm
Volume^Porod	51	nm³

SASDD55 – ...toxin-antitoxin system HicB family antitoxin - HicB protein - bound to addiction module toxin, HicA

Type II toxin-antitoxin system HicB family antitoxinAddiction module toxin, HicA experimental SAS data

Sample:	...toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein ...toxin, HicA monomer, 7 kDa Burkholderia pseudomallei protein
Buffer:	25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2016 Jul 27

R_g^Guinier	3.2	nm
D_max	11.0	nm
Volume^Porod	110	nm³

SASDR55 – ...toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS bound to bile salt

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxSbile acid: sodium cholate hydrate experimental SAS data

Sample:	...toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein bile acid: sodium cholate hydrate monomer, 0 kDa
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jul 7

R_g^Guinier	2.4	nm
D_max	6.6	nm
Volume^Porod	60	nm³

SASDF85 – ...toxin HigA: apo PaHigA

Uncharacterized protein experimental SAS data

Sample:	Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
Buffer:	20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 21

...Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H

R_g^Guinier	2.0	nm
D_max	6.6	nm
Volume^Porod	23	nm³

SASDF95 – ...toxin HigA bound to duplex DNA: PaHigA-DNA complex

Uncharacterized proteinDNA Duplex experimental SAS data

Sample:	Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein DNA Duplex dimer, 20 kDa DNA
Buffer:	20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Sep 19

...Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H

R_g^Guinier	2.9	nm
D_max	9.8	nm
Volume^Porod	81	nm³

SASDGM5 – ...Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data

Sample:	...Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM DTT, 4 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 May 31

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A

R_g^Guinier	4.4	nm
D_max	15.5	nm
Volume^Porod	89	nm³

SASDGN5 – ...Toxin (CyaA)

Sample:	...Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 May 31

R_g^Guinier	8.3	nm
D_max	33.0	nm

SASDJN5 – ...toxin (TeNT/iHn)

Tetanus toxin (C467S) experimental SAS data

Isolated translocation domain of tetanus nerotoxin (TeNT/iHn) Rg histogram

Sample:	...toxin (C467S) monomer, 46 kDa Clostridium tetani protein
Buffer:	10 mM HEPES 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL45XU, SPring-8 on 2018 Oct 20

...toxin revealed by crystallographic and solution scattering analyses. J Struct Biol X 5:100045 (2021)
Zhang CM, Imoto Y, Hikima T, Inoue T

R_g^Guinier	3.0	nm
D_max	12.1	nm
Volume^Porod	54	nm³

SASDJP5 – ...toxin (TeNT/blHn)

Sample:	...toxin monomer, 35 kDa Clostridium tetani protein
Buffer:	10 mM HEPES 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL45XU, SPring-8 on 2018 Oct 20

...toxin revealed by crystallographic and solution scattering analyses. J Struct Biol X 5:100045 (2021)
Zhang CM, Imoto Y, Hikima T, Inoue T

R_g^Guinier	2.9	nm
D_max	11.3	nm
Volume^Porod	42	nm³

SASDNV5 – ...Toxin E at acidic pH

Botulinum Toxin Serotype E, endonegative experimental SAS data

Sample:	...Toxin Serotype E, endonegative monomer, 144 kDa Clostridium botulinum protein
Buffer:	10 mM sodium acetate (20 mM ionic strength), pH: 4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Oct 6

...toxin E. J Struct Biol :107876 (2022)
Lalaurie CJ, Splevins A, Barata TS, Bunting KA, Higazi DR, Zloh M, Spiteri VA, Perkins SJ, Dalby PA

R_g^Guinier	4.3	nm
D_max	15.2	nm
Volume^Porod	240	nm³

SASDS56 – ...toxin ABC transporter RtxB

RTX toxin transporter experimental SAS data

Sample:	...toxin transporter monomer, 28 kDa Kingella kingae ATCC … protein
Buffer:	100 mM HEPES, 10 % glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20

Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter Scientific Reports 14(1) (2024)
Anlauf M, Bilsing F, Reiners J, Spitz O, Hachani E, Smits S, Schmitt L

R_g^Guinier	2.2	nm
D_max	7.6	nm
Volume^Porod	48	nm³

SASDRV6 – Transcription regulator PaaR2

Phage repressor protein CI (C120S) experimental SAS data

Sample:	Phage repressor protein CI (C120S) octamer, 122 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 16

...toxin-antitoxin system
Marusa Prolic Kalinsek

R_g^Guinier	5.0	nm
D_max	16.8	nm
Volume^Porod	428	nm³

SASDNW6 – ...toxin from Photobacterium damselae subsp. piscicida

Apoptosis inducing protein experimental SAS data

Sample:	Apoptosis inducing protein monomer, 57 kDa Photobacterium damselae subsp. … protein
Buffer:	50 mM Hepes, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Apr 13

...toxin AIP56. Nat Commun 14(1):7431 (2023)
Lisboa J, Pereira C, Pinto RD, Rodrigues IS, Pereira LMG, Pinheiro B, Oliveira P, Pereira PJB, Azevedo JE, Durand D, Benz R, do Vale A, Dos Santos NMS

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	76	nm³

SASDF87 – ...toxin Receptor Cqm1 protein in 100% D2O

Binary toxin receptor Cqm1 protein experimental SAS data

Sample:	...toxin receptor Cqm1 protein dimer, 129 kDa synthetic construct protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24

Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
Sharma M, Aswal V, Kumar V, Chidambaram R

R_g^Guinier	2.8	nm
D_max	9.7	nm

SASDF97 – ...toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O

Binary toxin receptor Cqm1 proteinDeuterated BinB component of mosquito-larvicidal Binary toxin experimental SAS data

Sample:	...toxin receptor Cqm1 protein monomer, 65 kDa synthetic construct protein ...toxin monomer, 56 kDa synthetic construct protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 23

Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
Sharma M, Aswal V, Kumar V, Chidambaram R

R_g^Guinier	1.9	nm
D_max	7.3	nm

SASDFA7 – ...toxin in 100% D2O

Lysinibacillus Mosquito-larvicidal receptor binding component experimental SAS data

Sample:	Lysinibacillus Mosquito-larvicidal receptor binding component monomer, 53 kDa synthetic construct protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24

Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
Sharma M, Aswal V, Kumar V, Chidambaram R

R_g^Guinier	3.3	nm
D_max	9.9	nm

SASDLR7 – ...Toxin

Hc fragment of Tetanus toxin experimental SAS data

Sample:	...toxin dimer, 103 kDa Clostridium tetani (strain … protein
Buffer:	50 mM sodium phosphate buffer, 300 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2004 May 14

...toxin forms stable, concentration-dependent dimers via an intermolecular disulphide bond. J Mol Biol 365(1):123-34 (2007)
Qazi O, Bolgiano B, Crane D, Svergun DI, Konarev PV, Yao ZP, Robinson CV, Brown KA, Fairweather N

R_g^Guinier	4.0	nm
D_max	13.2	nm
Volume^Porod	150	nm³

SASDE48 – Toxin GraT

Putative killer protein, Toxin GraT. experimental SAS data

Putative killer protein, Toxin GraT. Kratky plot

Sample:	...Toxin GraT. monomer, 11 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Sep 21

...toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R

R_g^Guinier	1.5	nm
D_max	5.5	nm
Volume^Porod	18	nm³

SASDE58 – ...toxin GraT with antotoxin GraA

Putative killer protein, Toxin GraT.antitoxin GraA, antidote of toxin GraTPutative killer protein, Toxin GraT experimental SAS data

Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT Kratky plot

Sample:	...Toxin GraT. monomer, 11 kDa Pseudomonas putida protein ...toxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein ...Toxin GraT monomer, 11 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

...toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R

R_g^Guinier	2.3	nm
D_max	7.9	nm
Volume^Porod	63	nm³

SASDE68 – ...toxin GraA in complex with the grta operator

GraTA operator regionantitoxin GraA, antidote of toxin GraTatitoxin GraA, antidote of GraT. experimental SAS data

GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Kratky plot

Sample:	GraTA operator region monomer, 20 kDa DNA ...toxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein ...toxin GraA, antidote of GraT. dimer, 23 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Dec 17

...toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R

R_g^Guinier	2.9	nm
D_max	10.3	nm
Volume^Porod	94	nm³

SASDXM8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Apr 1

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	3.7	nm
D_max	15.4	nm
Volume^Porod	52	nm³

SASDXN8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 1 mM CaCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 1 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	2.0	nm
D_max	8.1	nm
Volume^Porod	38	nm³

SASDXP8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 10 mM KCl

Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 10 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Dec 12

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	3.7	nm
D_max	15.6	nm
Volume^Porod	50	nm³

SASDXQ8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 1 mM MgCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	3.5	nm
D_max	14.9	nm
Volume^Porod	49	nm³

SASDHR8 – ...toxin receptor (Cqm1) protein

Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data

Sample:	...toxin receptor protein (Neutral and basic amino acid transport protein rBAT) dimer, 129 kDa Culex quinquefasciatus protein
Buffer:	PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24

...Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V

R_g^Guinier	4.0	nm
D_max	9.2	nm
Volume^Porod	97	nm³

SASDXR8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 10 mM MgCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 10 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	3.8	nm
D_max	16.3	nm
Volume^Porod	61	nm³

SASDHS8 – Mosquito-larvicidal BinB protein

Binary larvicide subunit BinB experimental SAS data

Sample:	Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Buffer:	PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24

...Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V

R_g^Guinier	2.9	nm
D_max	9.7	nm
Volume^Porod	48	nm³

SASDXS8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 1 mM SrCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 1 mM SrCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	35	nm³

SASDHT8 – ...toxin receptor (Cqm1)

Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data

Sample:	Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein ...toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer:	PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24

...Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V

R_g^Guinier	4.6	nm
D_max	11.7	nm
Volume^Porod	290	nm³

SASDXT8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 2 mM SrCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 2 mM SrCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	2.7	nm
D_max	11.5	nm
Volume^Porod	34	nm³

SASDHU8 – ...toxin receptor (Cqm1)

Sample:	Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein ...toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 26

...Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V

R_g^Guinier	3.7	nm
D_max	12.9	nm
Volume^Porod	174	nm³

SASDXU8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 3 mM SrCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 3 mM SrCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	2.4	nm
D_max	11.0	nm
Volume^Porod	33	nm³

SASDXV8 – ...toxin domain Block Vof Bifunctional hemolysin/adenylate cyclase, 1 mM BaCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 1 mM BaCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	3.0	nm
D_max	13.1	nm
Volume^Porod	32	nm³

SASDXW8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 3 mM BaCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 3 mM BaCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	2.5	nm
D_max	10.9	nm
Volume^Porod	34	nm³

SASDXX8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 10 mM BaCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 10 mM BaCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	2.7	nm
D_max	11.7	nm
Volume^Porod	35	nm³

SASDXY8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 10 mM SrCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT, 10 mM SrCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	2.1	nm
D_max	10.1	nm
Volume^Porod	36	nm³

SASDXZ8 – ...toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 3 mM CaCl2

Sample:	...toxin domain Block V of adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer:	50 mM Tris, 5 mM DTT , 3 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Dec 12

...toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ

R_g^Guinier	2.0	nm
D_max	8.8	nm
Volume^Porod	38	nm³

SASDBA9 – ...toxin/antitoxin heterohexamer complex

Toxin CcdBAntitoxin CcdAToxin CcdB experimental SAS data

Sample:	Toxin CcdB dimer, 23 kDa Escherichia coli protein ...toxin CcdA dimer, 17 kDa Escherichia coli protein Toxin CcdB dimer, 23 kDa Escherichia coli protein
Buffer:	10 mM Tris 50 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jul 24

Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. Nucleic Acids Res 45(6):2937-2950 (2017)
Vandervelde A, Drobnak I, Hadži S, Sterckx YG, Welte T, De Greve H, Charlier D, Efremov R, Loris R, Lah J

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	92	nm³

SASDSH9 – ...toxin

Heat-labile enterotoxin B chain experimental SAS data

Sample:	...toxin B chain monomer, 36 kDa Clostridium perfringens protein
Buffer:	10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18

...toxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A

R_g^Guinier	2.7	nm
D_max	10.2	nm
Volume^Porod	46	nm³

SASDSJ9 – ...toxin

Sample:	...toxin B chain monomer, 33 kDa Clostridium perfringens protein
Buffer:	10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18

...toxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A

R_g^Guinier	2.6	nm
D_max	9.5	nm
Volume^Porod	40	nm³

SASDCK9 – ...Toxin (CyaA)

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A

R_g^Guinier	2.6	nm
D_max	9.1	nm
Volume^Porod	60	nm³

SASDBL9 – Putative DNA binding protein YeeF-CT from Bacillus subtilis (amino acids 528-669)

Putative DNA binding protein experimental SAS data

Sample:	Putative DNA binding protein dimer, 37 kDa Bacillus subtilis subsp. … protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 17

...toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property
Krishan Gopal

R_g^Guinier	2.7	nm
D_max	9.5	nm
Volume^Porod	55	nm³

SASDCL9 – ...Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

R_g^Guinier	2.9	nm
D_max	9.8	nm
Volume^Porod	78	nm³

SASDBM9 – YezG: a cognate immunity protein of YeeF from Bacillus subtilis

YezG, cognate immunity protein of YeeF experimental SAS data

Sample:	YezG, cognate immunity protein of YeeF monomer, 19 kDa Bacillus subtilis subsp. … protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 17

...toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property
Krishan Gopal

R_g^Guinier	2.1	nm
D_max	6.1	nm
Volume^Porod	46	nm³

SASDBN9 – Putative DNA binding protein YeeF-CT in complex with the cognate immunity protein YezG from Bacillus subtilis

Putative DNA binding proteinYezG, cognate immunity protein of YeeF experimental SAS data

Sample:	Putative DNA binding protein dimer, 37 kDa Bacillus subtilis subsp. … protein YezG, cognate immunity protein of YeeF monomer, 19 kDa Bacillus subtilis subsp. … protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 18

...toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property
Krishan Gopal

R_g^Guinier	4.0	nm
D_max	14.1	nm
Volume^Porod	115	nm³

SASDPP3 – ...toxin (CyaA)

Double-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data

RTX719. Double-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Double-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 15

...toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L

R_g^Guinier	3.4	nm
D_max	14.5	nm
Volume^Porod	67	nm³

SASDHW7 – ...toxin-antitoxin complex (mRNA endoribonuclease toxin LS : Antitoxin RnlB)

mRNA endoribonuclease toxin LSAntitoxin RnlB (C-terminal His-tagged) experimental SAS data

mRNA endoribonuclease toxin LS Antitoxin RnlB (C-terminal His-tagged) Kratky plot

Sample:	...toxin LS dimer, 80 kDa Escherichia coli protein ...toxin RnlB (C-terminal His-tagged) monomer, 15 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R

R_g^Guinier	3.5	nm
D_max	10.0	nm
Volume^Porod	161	nm³

SASDJY7 – ...toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH)

Modular nanotransporter with a melanocyte stimulating hormone ligand module experimental SAS data

A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH) Rg histogram

Sample:	Modular nanotransporter with a melanocyte stimulating hormone ligand module monomer, 70 kDa synthetic construct protein
Buffer:	150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Nov 24

Low-resolution structures of modular nanotransporters shed light on their functional activity Acta Crystallographica Section D Structural Biology 76(12):1270-1279 (2020)
Khramtsov Y, Vlasova A, Vlasov A, Rosenkranz A, Ulasov A, Ryzhykau Y, Kuklin A, Orekhov A, Eydlin I, Georgiev G, Gordeliy V, Sobolev A

R_g^Guinier	3.7	nm
D_max	13.3	nm
Volume^Porod	127	nm³

SASDAA9 – EcPaaA2-EcParE2His construct

Plasmid stabilization protein ParE Uncharacterized protein (Antitoxin) experimental SAS data

Sample:	Plasmid stabilization protein ParE 16-mer, 188 kDa Escherichia coli protein ...toxin) 16-mer, 135 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2014 Dec 9

...toxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R

R_g^Guinier	3.8	nm
D_max	16.2	nm
Volume^Porod	312	nm³

SASDPQ3 – ...toxin (CyaA)

Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data

RTX719-K860R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 16

...toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L

R_g^Guinier	5.0	nm
D_max	25.8	nm
Volume^Porod	178	nm³

SASDHX7 – ...toxin LS)

mRNA endoribonuclease toxin LS experimental SAS data

mRNA endoribonuclease toxin LS Kratky plot

Sample:	...toxin LS dimer, 80 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	10.7	nm
Volume^Porod	134	nm³

SASDJZ7 – ...toxin/flavohemoprotein nanotransporter (MNT) fused to an epidermal growth factor ligand module (MNT-EGF)

Modular nanotransporter with an epidermal growth factor ligand module experimental SAS data

Sample:	Modular nanotransporter with an epidermal growth factor ligand module monomer, 76 kDa synthetic construct protein
Buffer:	150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Nov 24

R_g^Guinier	4.2	nm
D_max	16.0	nm
Volume^Porod	161	nm³

SASDAB9 – EcPaaA2-HisEcParE2 construct

Sample:	Plasmid stabilization protein ParE octamer, 102 kDa Escherichia coli protein ...toxin) octamer, 68 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Feb 5

...toxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R

R_g^Guinier	3.3	nm
D_max	15.3	nm
Volume^Porod	166	nm³

SASDKL2 – ...toxin LS from Escherichia coli (strain K12)

NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data

Sample:	NRD-HEPN truncated variant of RnlA endoribonuclease dimer, 62 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 27

R_g^Guinier	3.0	nm
D_max	10.4	nm
Volume^Porod	88	nm³

SASDPR3 – ...toxin (CyaA)

RTX719-K983R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 17

...toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L

R_g^Guinier	5.3	nm
D_max	26.3	nm
Volume^Porod	187	nm³

SASDC84 – ...toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein experimental SAS data

parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct Rg histogram

Sample:	Plasmid stabilization protein ParE monomer, 13 kDa Escherichia coli protein Uncharacterized protein monomer, 6 kDa Escherichia coli O157:H7 protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Feb 5

...toxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R

R_g^Guinier	2.2	nm
D_max	9.3	nm
Volume^Porod	36	nm³

SASDPS3 – ...toxin (CyaA)

Non-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data

proRTX719. Non-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Non-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 22

...toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L

R_g^Guinier	4.2	nm
D_max	17.8	nm
Volume^Porod	133	nm³

SASDHY7 – ...toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot

Sample:	...toxin LS - R255A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	20.1	nm
Volume^Porod	133	nm³

SASDKS9 – ...toxin LS (D245R mutant); batch-SAXS measurements in low salt buffer

mRNA endoribonuclease toxin LS (D245R mutant) experimental SAS data

Sample:	...toxin LS (D245R mutant) dimer, 84 kDa Escherichia coli (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	4.3	nm
D_max	15.9	nm
Volume^Porod	183	nm³

SASDHZ7 – ...toxin LS) - R318A single alanine mutant

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged Kratky plot

Sample:	...toxin LS - R318A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.4	nm
D_max	12.7	nm
Volume^Porod	124	nm³

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